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AB4051

Anti-Caspase-3 antibody

4

(7 Reviews)

|

(377 Publications)

Anti-Caspase-3 antibody (ab4051) is a rabbit polyclonal antibody detecting Caspase-3 in Western Blot, IHC-P. Suitable for Human.

- Over 310 publications
- Trusted since 2003

View Alternative Names

CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (AB4051)
  • IHC-P

AbReview45427****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (AB4051)

ab4051 staining Caspase 3 in Pig liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 20 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

Image courtesy of an anonymous AbReview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (AB4051)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (AB4051)

ab4051 staining Caspase-3 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (AB4051)
  • IHC-P

AbReview45427****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (AB4051)

ab4051 staining Caspase 3 in Cow Ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 15 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/75 in PBS + BSA) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

Image courtesy of an anonymous AbReview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (AB4051)
  • IHC-P

AbReview81502****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (AB4051)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde-fixed human brain cortex tissue staining with ab4051 at 1/200 dilution. Secondary antibody was HRP Agilent anti-mouse/rabbit EnVision Dual Link. Samples were incubated with the primary antibody with Agilent antibody diluent for 14 hours at 4°C. Blocking was done using 3% serum for 1 hour at 22°C. Heat mediated antigen retrieval with pH = 6 citrate.

This image is courtesy of an anonymous Abreview.

Western blot - Anti-Caspase-3 antibody (AB4051)
  • WB

Supplier Data

Western blot - Anti-Caspase-3 antibody (AB4051)

All lanes:

Western blot - Anti-Caspase-3 antibody (ab4051)

All lanes:

Human Lung Cell Extract

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CASP3 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P42574

Specificity

<p>Caspase-3 is a member of the interleukin-1 β-converting enzyme family. Caspase-3 is thought to be associated with induction of apoptosis. Caspase-3 is synthesized as inactive 32 kDa proenzyme and is processed during apoptosis generating two subunits of 17 kDa and 12 kDa. Caspase-3 stains the epithelial cells of skin, renal proximal tubules and collecting ducts.<br><br>This antibody reacts with the 32 kDa proenzyme and the 17 kDa active form of caspase 3&#46;<br>&nbsp;</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.002 mg/mL", "IHCP-species-notes": "<p>Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Anti-Caspase-3 antibody (ab4051) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in human samples.

Anti-Caspase-3 antibody (ab4051) has been cited over 317 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Caspase-3 antibody (ab4051) has high sensitivity and specificity.

Anti-Caspase-3 antibody (ab4051) has 8 independent reviews from customers.

Anti-Caspase-3 antibody (ab4051) specifically detects Caspase-3 (UniProt ID: P42574; Molecular weight: 17kDa) and is sold in a 500 µL selling size.
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
Purified immunoglobulin fraction of rabbit antiserum against Caspase-3 containing sodium azide as a preservative.
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 35338844, PubMed : 35446120, PubMed : 7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed : 10497198, PubMed : 16374543, PubMed : 7596430, PubMed : 7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed : 7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed : 37993714, PubMed : 9334240). Involved in the cleavage of huntingtin (PubMed : 8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed : 21357690). Cleaves DSG2 in response to apoptosis resulting in a loss of full length DSG2 at desmosome cell junctions and subsequent loss of cell-cell adhesion (PubMed : 17559062). Also cleaves JUP in response to apoptosis (PubMed : 17559062). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed : 23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed : 30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed : 35338844, PubMed : 35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed : 23845944, PubMed : 33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758104, PubMed : 36758106).
See full target information CASP3
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Publications (377)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:33991 PubMed41028062

2025

SARS-CoV-2 spike protein-induced inflammation underlies proarrhythmia in COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Louisa Mezache,Andrew Soltisz,Esmerina Tili,Gerard J Nuovo,Rengasayee Veeraraghavan

Life (Basel, Switzerland) 15: PubMed41010372

2025

A Dose-Dependent Study Examining Dexmedetomidine's Possible Effects Against Oxidative, Fibrotic, and Apoptotic Damage Induced by Radiation Exposure in Spleen Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Hatice Beyazal Polat,Hamit Yılmaz,Kagan Kilinc,Belemir Gülhan,Sema Yılmaz Rakıcı,Levent Tümkaya

Radiology and oncology 59:349-367 PubMed40959921

2025

The role of focal adhesion kinase in bladder cancer: translation from to human urothelial carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Gaja Markovic,Natasa Resnik,Aleksandar Janev,Dasa Zupancic,Gasper Grubelnik,Marusa Debeljak,Maja Cemazar,Tanja Jesenko,Masa Omerzel,Tomaz Smrkolj,Mateja Erdani Kreft

Scientific reports 15:32527 PubMed40954150

2025

Co-administration of Spirulina and L-carnitine preserves ovarian reserve in a rat model of premature ovarian insufficiency via SIRT1 regulation of oxidative stress, inflammation, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eman M Embaby,Gehad E Elshopakey,Aya Megahed,Shaymaa Rezk,Ahmed Ateya,Mamdouh Eldesoqui,Eman H Yousef,Mona M Elghareeb

International journal of molecular sciences 26: PubMed40869093

2025

An Investigation of the Effects of Melatonin and Vitamin D on the Ovaries of a Rat Model of Premature Ovarian Failure Induced by Cyclophosphamide.

Applications

Unspecified application

Species

Unspecified reactive species

Özdem Karaoğlan,Yurdun Kuyucu,Dilek Şaker,Gülçin Dağlıoğlu,Özgül Tap

Plants (Basel, Switzerland) 14: PubMed40805798

2025

Exploring the Bioactive Potential and Chemical Profile of Essential Oil: An Integrated and Evaluation.

Applications

Unspecified application

Species

Unspecified reactive species

Rómulo Oses,Matías Ferrando,Flavia Bruna,Patricio Retamales,Myriam Navarro,Katia Fernández,Waleska Vera,María José Larrazábal,Iván Neira,Adrián Paredes,Manuel Osorio,Osvaldo Yáñez,Martina Jacobs,Jessica Bravo

Drug development research 86:e70136 PubMed40785696

2025

Design, Synthesis, Biological Evaluation, and Computational Studies of Novel 1,4-Diketopiperazines as GABA Agonist.

Applications

Unspecified application

Species

Unspecified reactive species

Mostafa Abd El-Mohsen Anwar,Eman R Mohammed,Samir M El Moghazy,Nesma M E Abo El Nasr,Marawan A Elbaset,Reda M S Korany,Omar Ahmed-Farid,Ibrahim M Ibrahim,Walaa Hamada Abd-Allah

Naunyn-Schmiedeberg's archives of pharmacology : PubMed40679589

2025

Cerium oxide nanoparticles attenuate hepatic failure via blocking TGF-β/Smads and upregulating Nrf2/HO-1 signaling pathways in liver fibrosis rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Noha A Mowaad,Sara M Baraka,Saber Ibrahim,Doaa A Mansour,Reda M S Korany,Ahmed F El-Sayed,Arwa A Hassan

Discover oncology 16:1327 PubMed40658186

2025

Unveiling the anticancer potential of flavonoids in hepatocellular carcinoma through microbiome and spatially resolved metabolomics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Lina You,WuKui Huang,ShuYing Chen

NPJ breast cancer 11:67 PubMed40624019

2025

NR4A3 potentials M1-like macrophage polarization to facilitate anti-tumor immune responses in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Yu Qian,Ning Jin,Shan-Shan Rao,Ya Wang,Xin Li,Wen Pan,Pu Huang,Si-Yuan Wang,Ping-Fei Li,Yan-Kai Lv,Qing-Lei Gao,Yu Xia
View all publications
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Product promise

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