Mouse Monoclonal Caspase-3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CASP3.
Images
![Western blot - Anti-Caspase-3 antibody [ABM1C12] (AB208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--western-blot-img53954.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-3 antibody [ABM1C12] (AB208161)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87424.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130410.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img35063.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130952.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)")
Publications
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- Journal of veterinary research 67:307-3142023Functional and morphological maturation of the full-sized and mini-pig corpus luteum by programmed cell death mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Hye Lee,Sang-Hwan KimPubMed 38143820
- Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES 28:885-8932022Effects of Carvedilol on liver ischemia-reperfusion injury in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMehmet Tolga Kafadar,Mehmet Ali GökPubMed 35775675
- Molecular medicine reports 25:2022Exenatide exerts a neuroprotective effect against diabetic cognitive impairment in rats by inhibiting apoptosis: Role of the JNK/c‑JUN signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGengyin Wang,Zongquan Zhao,Bo Ren,Wu Yu,Xudong Zhang,Jiang Liu,Liping Wang,Daowen Si,Meiliu YangPubMed 35119079
- Translational vision science & technology 10:102021Efficacy, Toxicity, and Pharmacokinetics of Intra-Arterial Chemotherapy Versus Intravenous Chemotherapy for Retinoblastoma in Animal Models and Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnthony B Daniels,Michael T Froehler,Jessica V Kaczmarek,Carley M Bogan,Pranav R Santapuram,Janene M Pierce,Sheau-Chiann Chen,Emma A Schremp,Kelli L Boyd,Yuankai K Tao,Marion W Calcutt,Tatsuki Koyama,Ann Richmond,Debra L FriedmanPubMed 34495330
- Translational vision science & technology 10:322021A Novel Rabbit Dry Eye Model Induced by a Controlled Drying System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Min Chen,Jian-Biao Kuang,Hui-Yin Yu,Zhen-Ning Wu,Shu-Yi Wang,Shi-You ZhouPubMed 34004007
- Clinical and experimental hepatology 7:215-2222021Histopathologic and electron microscopic investigation of the damage to liver tissue caused by parenteral nutrition combined with starvation in rabbits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSemra Gürünlüoğlu,Mehmet Gül,Harika Gözükara BağPubMed 34295990
- Analytical cellular pathology (Amsterdam) 2021:96524202021MicroRNA-520a Suppresses Pathogenesis and Progression of Non-Small-Cell Lung Cancer through Targeting the RRM2/Wnt Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Xie,Congyu Xue,Shuai Guo,Lei YangPubMed 33859925
- Cancer management and research 12:10829-108392020Ultrasound-Targeted Microbubble Destruction Mediated si-CyclinD1 Inhibits the Development of Hepatocellular Carcinoma via Suppression of PI3K/AKT Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Yan et. al.PubMed 33149688
- American journal of translational research 12:5252-52682020Atorvastatin suppresses the progression of cervical cancer via regulation of autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Sheng et. al.PubMed 33042417
- Experimental and molecular pathology 108:114-1202019Ailanthone up-regulates miR-449a to restrain acute myeloid leukemia cells growth, migration and invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Zhang et. al.PubMed 31002772
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CASP3. Database link P42574
Abcam Recommends
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Hamster | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Hamster | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120, PubMed:7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:10497198, PubMed:16374543, PubMed:7596430, PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed:7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed:37993714, PubMed:9334240). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35338844, PubMed:35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106).
Alternative names
CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1
Recommended products
Mouse Monoclonal Caspase-3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CASP3.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CASP3. Database link P42574
- Clone number
- ABM1C12
- Purification technique
- Affinity purification Protein G
- Specificity
ab208161 recognizes both pro- and active Caspase 3 after apoptosis has been induced in wildtype cells and not knockout cells
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--western-blot-img53954.jpg "Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161)")
Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161)
Lane 1: Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h)
Lane 2: Wild-type HAP1 cell lysate
Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h)
Lane 4: Caspase-3 knockout HAP1 cell lysate
Lanes 1 - 4: Merged signal (red and green). Green - ab208161 observed at 35 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
ab208161 was shown to recognise pro + active Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (± Staurosporine treatment) were subjected to SDS-PAGE. ab208161 at a concentration of 2 μg/ml and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) at a dilution of 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161)
Predicted band size: 31 kDa
Observed band size: 16 kDa, 18 kDa, 32 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87424.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human adenocarcinoma of rectum tissue labeling active + pro Caspase 3 with ab208161 at 5 μg/ml .
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130410.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon tissue labeling active + pro Caspase 3 with ab208161 at 5 μg/ml .
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img35063.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human spleen tissue labeling active + pro Caspase 3 with ab208161 at 5 μg/ml .
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130952.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human squamous cell carcinoma of lung tissue labeling active + pro Caspase 3 with ab208161 at 5 μg/ml .
![Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--western-blot-img95225.jpg "Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161)")
Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161)
All lanes: Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161) at 2 µg/mL
All lanes: Ramos cell lysate
Predicted band size: 31 kDa
![Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161), expandable thumbnail](https://content.abcam.com/products/images/caspase-3-antibody-abm1c12-ab208161--western-blot-img95225.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161)")
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