JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB208161

Anti-Caspase-3 antibody [ABM1C12]

Be the first to review this product! Submit a review

|

(25 Publications)

Mouse Monoclonal Caspase-3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 25 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CASP3.

View Alternative Names

CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human spleen tissue labeling active + pro Caspase 3 with ab208161 at 5 μg/ml .

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human squamous cell carcinoma of lung tissue labeling active + pro Caspase 3 with ab208161 at 5 μg/ml .

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human adenocarcinoma of rectum tissue labeling active + pro Caspase 3 with ab208161 at 5 μg/ml .

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (AB208161)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon tissue labeling active + pro Caspase 3 with ab208161 at 5 μg/ml .

Western blot - Anti-Caspase-3 antibody [ABM1C12] (AB208161)
  • WB

Lab

Western blot - Anti-Caspase-3 antibody [ABM1C12] (AB208161)

Lane 1 : Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h)
Lane 2 : Wild-type HAP1 cell lysate
Lane 3 : Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h)
Lane 4 : Caspase-3 knockout HAP1 cell lysate
Lanes 1 - 4 : Merged signal (red and green). Green - ab208161 observed at 35 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab208161 was shown to recognise pro + active Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (± Staurosporine treatment) were subjected to SDS-PAGE. ab208161 at a concentration of 2 μg/ml and ab181602 (loading control to GAPDH) at a dilution of 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161)

Predicted band size: 31 kDa

Observed band size: 16 kDa,18 kDa,32 kDa

false

Western blot - Anti-Caspase-3 antibody [ABM1C12] (AB208161)
  • WB

Supplier Data

Western blot - Anti-Caspase-3 antibody [ABM1C12] (AB208161)

All lanes:

Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161) at 2 µg/mL

All lanes:

Ramos cell lysate

Predicted band size: 31 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ABM1C12

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CASP3.

P42574

Specificity

ab208161 recognizes both pro- and active Caspase 3 after apoptosis has been induced in wildtype cells and not knockout cells

style
left-column

Alternative Products

Primary Antibodies

AB4051

Anti-Caspase-3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (AB4051)

  • 4
  • 7
Primary Antibodies

AB13847

Anti-Caspase-3 antibody

KO Validated

Western blot - Anti-Caspase-3 antibody (AB13847)

  • 4
  • 56
Primary Antibodies

AB195905

Biotin Anti-Caspase-3 antibody [E87]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Caspase-3 antibody [E87] (AB195905)

  • 0
  • 0
Primary Antibodies

AB184787

Anti-Caspase-3 antibody [EPR18297]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [EPR18297] (AB184787)

  • 3
  • 2
Primary Antibodies

AB32351

Anti-Caspase-3 antibody [E87]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (AB32351)

  • 4
  • 15
Primary Antibodies

AB224271

Anti-Caspase-3 antibody [EPR18297] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [EPR18297] - BSA and Azide free (AB224271)

  • 0
  • 0
Primary Antibodies

AB197202

Anti-Caspase-3 antibody [E87] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Caspase-3 antibody [E87] - BSA and Azide free (AB197202)

  • 0
  • 0

Complementary Products

Assay Kits

AB39401

Caspase-3 Assay Kit (Colorimetric)

Functional Studies - Caspase-3 Assay Kit (Colorimetric) (AB39401)

  • 0
  • 1
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 35338844, PubMed : 35446120, PubMed : 7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed : 10497198, PubMed : 16374543, PubMed : 7596430, PubMed : 7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed : 7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed : 37993714, PubMed : 9334240). Involved in the cleavage of huntingtin (PubMed : 8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed : 21357690). Cleaves DSG2 in response to apoptosis resulting in a loss of full length DSG2 at desmosome cell junctions and subsequent loss of cell-cell adhesion (PubMed : 17559062). Also cleaves JUP in response to apoptosis (PubMed : 17559062). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed : 23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed : 30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed : 35338844, PubMed : 35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed : 23845944, PubMed : 33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758104, PubMed : 36758106).
See full target information CASP3
style
left-column

Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:28086 PubMed39543224

2024

Enhancing the wound healing potential using earthworm clitellum factors and elucidating its molecular mechanism in an in-vitro and earthworm model.

Applications

Unspecified application

Species

Unspecified reactive species

Kamarajan Rajagopalan,Jackson Durairaj Selvan Christyraj,Johnson Retnaraj Samuel Selvan Christyraj,Meikandan Chandrasekar,Nivedha Balamurugan,Nandha Kumar Suresh,Puja Das,Ashwin Barath Vaidhyalingham,Leela Bharathiraja

International journal of clinical and experimental pathology 17:371-380 PubMed39544716

2024

PCSK7, a potential target for the treatment of age-related macular degeneration: inhibition of retinal epithelial cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Zhang,Xin Zhao,Xiangyang Xin

Acta clinica Croatica 62:437-446 PubMed39310681

2024

DOES GALLIC ACID HAVE A POTENTIAL REMEDIAL EFFECT IN EXPERIMENTAL CORROSIVE BURN INJURY TO THE ESOPHAGUS?

Applications

Unspecified application

Species

Unspecified reactive species

Erol Basuguy,Ebru Gokalp Ozkorkmaz

Scientific reports 14:18283 PubMed39112499

2024

The role of protein prenylation inhibition through targeting FPPS by zoledronic acid in the prevention of renal fibrosis in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Reham Hussein Mohamed,Dina S Abdelrahim,Nesma Hussein Abdel Hay,Nesma Mohamed Fawzy,Doaa Karem M M,Dalia Ahmed Yousef Yehia,Omnia M AbdelMaksoud,Yomna M Tamim

Translational oncology 46:102006 PubMed38823259

2024

Succinylation of 14-3-3 theta by CPT1A promotes survival and paclitaxel resistance in nasal type extranodal natural killer/T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Cui,Chengcheng Cao,Xinyang Li,Biyan Lin,Aihui Yan,Ying Yang

Journal of cardiovascular development and disease 11: PubMed38248875

2024

Calciprotein Particles Induce Cellular Compartment-Specific Proteome Alterations in Human Arterial Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Shishkova,Arseniy Lobov,Egor Repkin,Victoria Markova,Yulia Markova,Anna Sinitskaya,Maxim Sinitsky,Egor Kondratiev,Evgenia Torgunakova,Anton Kutikhin

Journal of veterinary research 67:307-314 PubMed38143820

2023

Functional and morphological maturation of the full-sized and mini-pig corpus luteum by programmed cell death mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hye Lee,Sang-Hwan Kim

International journal of molecular sciences 23: PubMed36499266

2022

Calciprotein Particles Cause Physiologically Significant Pro-Inflammatory Response in Endothelial Cells and Systemic Circulation.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Shishkova,Arseniy Lobov,Bozhana Zainullina,Vera Matveeva,Victoria Markova,Anna Sinitskaya,Elena Velikanova,Maxim Sinitsky,Anastasia Kanonykina,Yulia Dyleva,Anton Kutikhin

Heliyon 8:e11529 PubMed36439719

2022

Regulation of the apoptosis/autophagy switch by propionic acid in ventromedial hypothalamus of rats with type 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Larysa Natrus,Yuliia Osadchuk,Olha Lisakovska,Toralf Roch,Nina Babel,Yuliia Klys,Dmytro Labudzynskyi,Yuri Chaikovsky

Microbiology spectrum 10:e0150922 PubMed36326498

2022

Ubiquitination of SARS-CoV-2 ORF7a Prevents Cell Death Induced by Recruiting BclXL To Activate ER Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Liu,Yanan Fu,Yanping Huang,Feng Zeng,Jingjing Rao,Xiao Xiao,Xiaoguang Sun,Hao Jin,Jian Li,Jing Yang,Weixing Du,Long Liu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com