Rabbit Recombinant Monoclonal Caspase-3 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples. Cited in 2 publications.
IgG
Rabbit
Constituents: 100% PBS
Liquid
Monoclonal
| sELISA | |
|---|---|
| Mouse | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes (By similarity). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:12124386).
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, LICE, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, Cpp32, Casp3
Rabbit Recombinant Monoclonal Caspase-3 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples. Cited in 2 publications.
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, LICE, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1, Cpp32, Casp3
IgG
Rabbit
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR17664-153
Affinity purification Protein A
Blue Ice
+4°C
ab281235 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Caspase 3(total). The recommended pair for sandwich ELISA is:
Capture: ab281235, Mouse Caspase 3(total) Capture Antibody (unconjugated)
Detector: Anti-Caspase-3 antibody [EPR17664-28] - BSA and Azide free (Detector) ab281085, Mouse Caspase 3(total) Detector Antibody (unconjugated)
The reference range value is 23 - 1500 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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