Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (AB247301)

This data was developed using ab40887, the same antibody clone in a different buffer formulation.

Stainign of Caspase 5 on paraffin-embedded human breast carcinoma sections, using ab40557 at a 1/50 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Lab

Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (AB247301)

This data was developed using ab40887, the same antibody clone in a different buffer formulation.
Purified ab40887 at 1/50 dilution (2μg) immunoprecipitating Caspase-5 in HeLa treated with heat shock (42°C 1h) then recovery for 6h whole cell lysate.
Blocking and diluting Buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] (<a href='/en-us/products/primary-antibodies/caspase-5-antibody-ep876y-ab40887'>ab40887</a>)

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat shock (42&deg;C 1h) then recovery for 6h whole cell lysate (Input) at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/caspase-5-antibody-ep876y-ab40887'>ab40887</a> + HeLa treated with heat shock (42&deg;C, 1h) then recovery for 6h whole cell lysate

Lane 3:

<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> instead of <a href='/en-us/products/primary-antibodies/caspase-5-antibody-ep876y-ab40887'>ab40887</a> in HeLa treated with heat shock (42&deg;C 1h) then recovery for 6h whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 47 kDa

false

• WB

Unknown

Western blot - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (AB247301)

This data was developed using ab40887, the same antibody clone in a different buffer formulation.

The cell lysates were prepared in 1%SDS Hot lysis method.

All lanes:

Western blot - Anti-Caspase-5 antibody [EP876Y] (<a href='/en-us/products/primary-antibodies/caspase-5-antibody-ep876y-ab40887'>ab40887</a>) at 1/10000 dilution

All lanes:

HeLa cell lysate at 10 µg

Predicted band size: 50 kDa

Observed band size: 47 kDa

false

• WB

Lab

Western blot - Anti-Caspase-5 antibody [EP876Y] - BSA and Azide free (AB247301)

This data was developed using ab40887, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Caspase-5 antibody [EP876Y] (<a href='/en-us/products/primary-antibodies/caspase-5-antibody-ep876y-ab40887'>ab40887</a>) at 1/1000 dilution

All lanes:

HT-1080 (Human fibrosarcoma epithelial cell) prepared in 1%SDS Hot lysis method whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 47 kDa

false