Rabbit Recombinant Monoclonal Caspase-6/CASP-6 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (AB240296), expandable thumbnail](https://content.abcam.com/products/images/caspase-6-casp-6-antibody-epr18043-bsa-and-azide-free-ab240296--immunocytochemistry-immunofluorescence-img33950.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (AB240296)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (AB240296), expandable thumbnail](https://content.abcam.com/products/images/caspase-6-casp-6-antibody-epr18043-bsa-and-azide-free-ab240296--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131567.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (AB240296)")
![Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (AB240296), expandable thumbnail](https://content.abcam.com/products/images/caspase-6-casp-6-antibody-epr18043-bsa-and-azide-free-ab240296--immunoprecipitation-img9387.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (AB240296)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (AB240296), expandable thumbnail](https://content.abcam.com/products/images/caspase-6-casp-6-antibody-epr18043-bsa-and-azide-free-ab240296--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113492.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (AB240296)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (AB240296), expandable thumbnail](https://content.abcam.com/products/images/caspase-6-casp-6-antibody-epr18043-bsa-and-azide-free-ab240296--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79180.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (AB240296)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Predicted | Expected | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity (PubMed:19133298, PubMed:22858542, PubMed:27032039, PubMed:28864531, PubMed:30420425, PubMed:32298652, PubMed:8663580). Acts as a non-canonical executioner caspase during apoptosis: localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation (PubMed:11953316, PubMed:17401638, PubMed:8663580, PubMed:9463409). Lamin-A/LMNA cleavage is required for chromatin condensation and nuclear disassembly during apoptotic execution (PubMed:11953316). Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis (PubMed:32029622). Cleaves PARK7/DJ-1 in cells undergoing apoptosis (By similarity). Involved in intrinsic apoptosis by mediating cleavage of RIPK1 (PubMed:22858542). Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP (PubMed:10559921, PubMed:14657026). Cleaves phospholipid scramblase proteins XKR4 and XKR9 (By similarity). In addition to apoptosis, involved in different forms of programmed cell death (PubMed:32298652). Plays an essential role in defense against viruses by acting as a central mediator of the ZBP1-mediated pyroptosis, apoptosis, and necroptosis (PANoptosis), independently of its cysteine protease activity (PubMed:32298652). PANoptosis is a unique inflammatory programmed cell death, which provides a molecular scaffold that allows the interactions and activation of machinery required for inflammasome/pyroptosis, apoptosis and necroptosis (PubMed:32298652). Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death (PubMed:32298652). Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity). Regulates B-cell programs both during early development and after antigen stimulation (By similarity). (Microbial infection) Proteolytically cleaves the N protein of coronaviruses such as MERS-CoV and SARS-CoV (PubMed:18155731, PubMed:35922005). The cleavage of MERS-CoV N-protein leads to two fragments and modulates coronavirus replication by regulating IFN signaling. The two fragments produced by the cleavage interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes (PubMed:35922005). The same mechanism seems to be used by other coronaviruses such as SARS-CoV and SARS-CoV-2 to enhance their replication (PubMed:35922005).
Alternative names
MCH2, CASP6, Caspase-6, CASP-6, CSP-6, Apoptotic protease Mch-2
Recommended products
Rabbit Recombinant Monoclonal Caspase-6/CASP-6 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18043
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240296 is the carrier-free version of Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296), expandable thumbnail](https://content.abcam.com/products/images/caspase-6-casp-6-antibody-epr18043-bsa-and-azide-free-ab240296--immunocytochemistry-immunofluorescence-img33950.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296)")
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Caspase-6/CASP-6 with Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing both nuclear and cytoplasmic staining on Jurkat cells. The staining remained similar after treatment with staurosporine (1uM, 4 hours) as the antibody interacts with the subunit p11.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296), expandable thumbnail](https://content.abcam.com/products/images/caspase-6-casp-6-antibody-epr18043-bsa-and-azide-free-ab240296--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131567.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Caspase-6/CASP-6 with Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Cytoplasmic and nuclear staining on epithelial cells of human colon is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296), expandable thumbnail](https://content.abcam.com/products/images/caspase-6-casp-6-antibody-epr18043-bsa-and-azide-free-ab240296--immunoprecipitation-img9387.jpg "Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296)")
Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296)
Caspase-6/CASP-6 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) treated with 1uM staurosporine for 4 hours whole cell lysate with Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645 at 1/50 dilution. Western blot was performed from the immunoprecipitate using Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/1500 dilution.
Lane 1: NIH/3T3 treated with 1uM staurosporine for 4 hours whole cell lysate10 μg (Input). Lane 2: Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645 IP in NIH/3T3 treated with 1uM staurosporine for 4 hours whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645 in NIH/3T3 treated with 1uM staurosporine for 4 hours whole cell lysate.
The cleaved Caspase-6/CASP-6 appears slightly larger than 11kDa. This fragment contains subunit p11 plus the internal propeptide.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645).
All lanes: Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] (Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645)
Predicted band size: 33 kDa
Observed band size: 11 kDa, 33 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296), expandable thumbnail](https://content.abcam.com/products/images/caspase-6-casp-6-antibody-epr18043-bsa-and-azide-free-ab240296--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113492.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296)
Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Caspase-6 / CASP-6 with Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Cytoplasmic staining on neurons of mouse brain is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296), expandable thumbnail](https://content.abcam.com/products/images/caspase-6-casp-6-antibody-epr18043-bsa-and-azide-free-ab240296--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79180.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free (ab240296)
Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Caspase-6/CASP-6 with Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Cytoplasmic staining on neurons of rat brain is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caspase-6/CASP-6 antibody [EPR18043] ab185645).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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