Rabbit Monoclonal Caspase-7 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 24 publications.
Images
![Western blot - Anti-Caspase-7 antibody [E22] (AB32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--western-blot-img25201.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-7 antibody [E22] (AB32522)")
![Western blot - Anti-Caspase-7 antibody [E22] (AB32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--western-blot-img100168.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-7 antibody [E22] (AB32522)")
![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (AB32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--immunocytochemistry-immunofluorescence-img21816.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (AB32522)")
![Immunoprecipitation - Anti-Caspase-7 antibody [E22] (AB32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--immunoprecipitation-img7787.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caspase-7 antibody [E22] (AB32522)")
Publications
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- International journal of oncology 60:2022Visfatin inhibits colon cancer cell apoptosis and decreases chemosensitivity to 5‑FU by promoting the SDF‑1/CXCR4/Akt axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQuan Zhao,Yaxin Long,Wen Cheng,Yingguang Huang,Jinyuan Li,Yuejin Li,Xing Li,Xiaodong Guo,Yu Li,Guosan Li,Kunmei Gong,Jian ZhangPubMed 35506454
- Acta pharmaceutica Sinica. B 12:2268-22792022Anticarin- shows a promising anti-osteosarcoma effect by specifically inhibiting CCT4 to impair proteostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGan Wang,Min Zhang,Ping Meng,Chengbo Long,Xiaodong Luo,Xingwei Yang,Yunfei Wang,Zhiye Zhang,James Mwangi,Peter Muiruri Kamau,Zhi Dai,Zunfu Ke,Yi Zhang,Wenlin Chen,Xudong Zhao,Fei Ge,Qiumin Lv,Mingqiang Rong,Dongsheng Li,Yang Jin,Xia Sheng,Ren LaiPubMed 35646538
- The Journal of international medical research 49:30006052110083252021FK506-binding protein 5 promotes the progression of papillary thyroid carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenya Gao,Fang Yu,Huanxia Jia,Zhuo Ye,Shijie YaoPubMed 33906532
- Journal of ophthalmology 2021:66940502021Casein Kinase 2-Interacting Protein-1 Alleviates High Glucose-Reduced Autophagy, Oxidative Stress, and Apoptosis in Retinal Pigment Epithelial Cells via Activating the p62/KEAP1/NRF2 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia Zhao,Jing Wang,Pei Li,Liying Tang,Yuzhi BaiPubMed 33628480
- Biological research 53:522020Caspase-7 deficiency in Chinese hamster ovary cells reduces cell proliferation and viability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatemeh Safari et. al.PubMed 33187557
- Molecular medicine reports 22:4810-48202020Exosomal circular RNA_400068 promotes the development of renal cell carcinoma via the miR‑210‑5p/SOCS1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Xiao et. al.PubMed 33173957
- Brazilian journal of medical and biological resear 53:e93602020MicroRNA-770-5p contributes to podocyte injury via targeting E2F3 in diabetic nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuanjuan Guo et. al.PubMed 32696822
- Neoplasma 67:547-5562020CBL0137 administration suppresses human hepatocellular carcinoma cells proliferation and induces apoptosis associated with multiple cell death related proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM A Hasan Albahde et. al.PubMed 32202904
- Acta biochimica et biophysica Sinica 52:371-3812020MIR205HG facilitates carcinogenesis of lung squamous cell carcinoma in vitro revealed by long noncoding RNA profiling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Chang,Xinying Xue,Chunsun Li,Wei Zhao,Yongfu Ma,Fei Xu,Zhen Wu,Yu Dai,Yunjing Li,Yang Liu,Liang'an ChenPubMed 32188965
- International journal of molecular medicine 45:1103-11112020A novel role for Livin in the response to ultraviolet B radiation and pterygium development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang-Qing Wu et. al.PubMed 32124942
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Thiol protease involved in different programmed cell death processes, such as apoptosis, pyroptosis or granzyme-mediated programmed cell death, by proteolytically cleaving target proteins (PubMed:11257230, PubMed:11257231, PubMed:11701129, PubMed:15314233, PubMed:16916640, PubMed:17646170, PubMed:18723680, PubMed:19581639, PubMed:8521391, PubMed:8567622, PubMed:8576161, PubMed:9070923). Has a marked preference for Asp-Glu-Val-Asp (DEVD) consensus sequences, with some plasticity for alternate non-canonical sequences (PubMed:12824163, PubMed:15314233, PubMed:17697120, PubMed:19581639, PubMed:20566630, PubMed:23650375, PubMed:23897474, PubMed:27032039). Its involvement in the different programmed cell death processes is probably determined by upstream proteases that activate CASP7 (By similarity). Acts as an effector caspase involved in the execution phase of apoptosis: following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of proteins, such as CLSPN, PARP1, PTGES3 and YY1 (PubMed:10497198, PubMed:16123041, PubMed:16374543, PubMed:16916640, PubMed:18723680, PubMed:20566630, PubMed:21555521, PubMed:22184066, PubMed:22451931, PubMed:27889207, PubMed:28863261, PubMed:31586028, PubMed:34156061, PubMed:35338844, PubMed:35446120). Compared to CASP3, acts as a minor executioner caspase and cleaves a limited set of target proteins (PubMed:18723680). Acts as a key regulator of the inflammatory response in response to bacterial infection by catalyzing cleavage and activation of the sphingomyelin phosphodiesterase SMPD1 in the extracellular milieu, thereby promoting membrane repair (PubMed:21157428). Regulates pyroptosis in intestinal epithelial cells: cleaved and activated by CASP1 in response to S.typhimurium infection, promoting its secretion to the extracellular milieu, where it catalyzes activation of SMPD1, generating ceramides that repair membranes and counteract the action of gasdermin-D (GSDMD) pores (By similarity). Regulates granzyme-mediated programmed cell death in hepatocytes: cleaved and activated by granzyme B (GZMB) in response to bacterial infection, promoting its secretion to the extracellular milieu, where it catalyzes activation of SMPD1, generating ceramides that repair membranes and counteract the action of perforin (PRF1) pores (By similarity). Following cleavage by CASP1 in response to inflammasome activation, catalyzes processing and inactivation of PARP1, alleviating the transcription repressor activity of PARP1 (PubMed:22464733). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (By similarity). Cleaves and activates sterol regulatory element binding proteins (SREBPs) (PubMed:8643593). Cleaves phospholipid scramblase proteins XKR4, XKR8 and XKR9 (By similarity). In case of infection, catalyzes cleavage of Kaposi sarcoma-associated herpesvirus protein ORF57, thereby preventing expression of viral lytic genes (PubMed:20159985). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106). Isoform Beta. Lacks enzymatic activity.
Alternative names
MCH3, CASP7, Caspase-7, CASP-7, Apoptotic protease Mch-3, CMH-1, ICE-like apoptotic protease 3, ICE-LAP3
Recommended products
Rabbit Monoclonal Caspase-7 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 24 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- E22
- Purification technique
- Affinity purification Protein A
- Specificity
The antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--western-blot-img25201.jpg "Western blot - Anti-Caspase-7 antibody [E22] (ab32522)")
Western blot - Anti-Caspase-7 antibody [E22] (ab32522)
Lanes 1- 2: Merged signal (red and green). Green - ab32522 observed at 38 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) observed at 50 kDa.ab32522 was shown to react with pro Caspase-7 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human CASP7 (Caspase-7) knockout HeLa cell line ab265777 (knockout cell lysate Human CASP7 (Caspase-7) knockout HeLa cell lysate ab257380) was used. Wild-type HeLa and CASP7 knockout HeLa cell lysates were subjected to SDS-PAGE. ab32522 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Caspase-7 antibody [E22] (ab32522) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: CASP7 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human CASP7 (Caspase-7) knockout HeLa cell line (Human CASP7 (Caspase-7) knockout HeLa cell line ab265777)
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 38 kDa
![Western blot - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--western-blot-img159565.gif "Western blot - Anti-Caspase-7 antibody [E22] (ab32522)")
Western blot - Anti-Caspase-7 antibody [E22] (ab32522)
All lanes: Western blot - Anti-Caspase-7 antibody [E22] (ab32522) at 1/1000 dilution
All lanes: Jurkat cell lysate
Predicted band size: 34 kDa
Observed band size: 34 kDa
![Western blot - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--western-blot-img100168.jpg "Western blot - Anti-Caspase-7 antibody [E22] (ab32522)")
Western blot - Anti-Caspase-7 antibody [E22] (ab32522)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: Wild type HAP1 + Staurosporine Staurosporine, Protein kinase inhibitor ab120056 whole cell lysate (20 μg)
Lane 3: CASP7 knockout HAP1 whole cell lysate (20 μg)
Lane 4: CASP7 + Staurosporine knockout HAP1 whole cell lysate (20 μg)
Lane 5: HeLa whole cell lysate (20 μg)
Lane 6: HeLa + Staurosporine whole cell lysate (20 μg)Lanes 1 - 6: Merged signal (red and green). Green - ab32522 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab32522 was shown to specifically react with HAP1 + Staurosporine when HAP1 + Staurosporine knockout samples were used. Wild-type and HAP1 + Staurosporine knockout samples were subjected to SDS-PAGE. ab32522 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Caspase-7 antibody [E22] (ab32522)
Predicted band size: 34 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--immunocytochemistry-immunofluorescence-img21816.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522)")
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522)
Immunofluorescent staining of HeLa cells using ab32522 at 1:100 dilution.
![Immunoprecipitation - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--immunoprecipitation-img7787.jpg "Immunoprecipitation - Anti-Caspase-7 antibody [E22] (ab32522)")
Immunoprecipitation - Anti-Caspase-7 antibody [E22] (ab32522)
Purified ab32522 at 1/20 dilution (1μg) immunoprecipitating Caspase-7 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+): ab32522 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab32522 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 34 kDaAll lanes: Immunoprecipitation - Anti-Caspase-7 antibody [E22] (ab32522)
Predicted band size: 34 kDa
![Flow Cytometry (Intracellular) - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--flow-cytometry-intracellular-img37356.jpg "Flow Cytometry (Intracellular) - Anti-Caspase-7 antibody [E22] (ab32522)")
Flow Cytometry (Intracellular) - Anti-Caspase-7 antibody [E22] (ab32522)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caspase-7 (red) with ab32522 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119089.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)
Immunohistochemical analysis of paraffin embedded human skin cancer tissue using ab32522 at 1:50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--flow-cytometry-intracellular-img37356.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Caspase-7 antibody [E22] (ab32522)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail](https://content.abcam.com/products/images/caspase-7-antibody-e22-ab32522--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119089.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)")
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