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Rabbit Monoclonal Caspase-7 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 24 publications.


Images

Western blot - Anti-Caspase-7 antibody [E22] (AB32522), expandable thumbnail
  • Western blot - Anti-Caspase-7 antibody [E22] (AB32522), expandable thumbnail
  • Western blot - Anti-Caspase-7 antibody [E22] (AB32522), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (AB32522), expandable thumbnail
  • Immunoprecipitation - Anti-Caspase-7 antibody [E22] (AB32522), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/20

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.

Alternative names

Caspase-7, CASP-7, Apoptotic protease Mch-3, CMH-1, ICE-like apoptotic protease 3, ICE-LAP3, CASP7, MCH3

Recommended products

Rabbit Monoclonal Caspase-7 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 24 publications.

Alternative names

Caspase-7, CASP-7, Apoptotic protease Mch-3, CMH-1, ICE-like apoptotic protease 3, ICE-LAP3, CASP7, MCH3

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

E22

Purification technique

Affinity purification Protein A

Specificity

The antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Western blot - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail

    Western blot - Anti-Caspase-7 antibody [E22] (ab32522)

    Lanes 1- 2: Merged signal (red and green). Green - ab32522 observed at 38 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) observed at 50 kDa.

    ab32522 was shown to react with pro Caspase-7 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human CASP7 (Caspase-7) knockout HeLa cell line ab265777 (knockout cell lysate Human CASP7 (Caspase-7) knockout HeLa cell lysate ab257380) was used. Wild-type HeLa and CASP7 knockout HeLa cell lysates were subjected to SDS-PAGE. ab32522 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Caspase-7 antibody [E22] (ab32522) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: CASP7 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 34 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail

    Western blot - Anti-Caspase-7 antibody [E22] (ab32522)

    All lanes: Western blot - Anti-Caspase-7 antibody [E22] (ab32522) at 1/1000 dilution

    All lanes: Jurkat cell lysate

    Predicted band size: 34 kDa

    Observed band size: 34 kDa

  • Western blot - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail

    Western blot - Anti-Caspase-7 antibody [E22] (ab32522)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: Wild type HAP1 + Staurosporine Staurosporine, Protein kinase inhibitor ab120056 whole cell lysate (20 μg)
    Lane 3: CASP7 knockout HAP1 whole cell lysate (20 μg)
    Lane 4: CASP7 + Staurosporine knockout HAP1 whole cell lysate (20 μg)
    Lane 5: HeLa whole cell lysate (20 μg)
    Lane 6: HeLa + Staurosporine whole cell lysate (20 μg)

    Lanes 1 - 6: Merged signal (red and green). Green - ab32522 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab32522 was shown to specifically react with HAP1 + Staurosporine when HAP1 + Staurosporine knockout samples were used. Wild-type and HAP1 + Staurosporine knockout samples were subjected to SDS-PAGE. ab32522 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Caspase-7 antibody [E22] (ab32522)

    Predicted band size: 34 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522)

    Immunofluorescent staining of HeLa cells using ab32522 at 1:100 dilution.

  • Immunoprecipitation - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail

    Immunoprecipitation - Anti-Caspase-7 antibody [E22] (ab32522)

    Purified ab32522 at 1/20 dilution (1μg) immunoprecipitating Caspase-7 in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
    Lane 2 (+): ab32522 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab32522 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 34 kDa

    All lanes: Immunoprecipitation - Anti-Caspase-7 antibody [E22] (ab32522)

    Predicted band size: 34 kDa

  • Flow Cytometry (Intracellular) - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Caspase-7 antibody [E22] (ab32522)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caspase-7 (red) with ab32522 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)

    Immunohistochemical analysis of paraffin embedded human skin cancer tissue using ab32522 at 1:50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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