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AB250510

Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free

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Rabbit Recombinant Monoclonal Caspase-7 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Mouse, Rat samples.

View Alternative Names

Lice2, Mch3, Casp7, Caspase-7, CASP-7, Apoptotic protease Mch-3, Cysteine protease LICE2

4 Images
Western blot - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (AB250510)
  • WB

Supplier Data

Western blot - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (AB250510)

This data was developed using ab181579, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Observed band size : 35kDa band is the pro-Caspase 7, 32kDa band is the N-terminal propeptide cleaved Caspase 7, and the 27kDa band is an N-terminal truncated caspase 7.

All lanes:

Western blot - Anti-Caspase-7 antibody [EPR17029] (<a href='/en-us/products/primary-antibodies/caspase-7-antibody-epr17029-ab181579'>ab181579</a>) at 1/5000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Lane 3:

Mouse spleen tissue lysate at 10 µg

Lane 4:

C6 (Rat glial tumor cells) lysate at 10 µg

Lane 5:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate at 10 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma) lysate at 10 µg

Lane 7:

NIH/3T3 (Mouse embyro fibroblast cells) lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 27 kDa,32 kDa,35 kDa

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Western blot - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (AB250510)
  • WB

Supplier Data

Western blot - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (AB250510)

This data was developed using ab181579, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Observed band size : The 35kDa band is the pro-caspase 7; 32kDa band is the N-terminal propeptide cleaved caspase 7; the 27kDa band is the truncated form and the 11kDa band is Caspase 7 subunit p11.

Lanes 1-4 were from the same blot; the exposure time for lanes 1-3 is 1 min and for lane 4 is 2 min.

All lanes:

Western blot - Anti-Caspase-7 antibody [EPR17029] (<a href='/en-us/products/primary-antibodies/caspase-7-antibody-epr17029-ab181579'>ab181579</a>) at 1/5000 dilution

Lane 1:

Rat brain tissue lysate at 10 µg

Lane 2:

Rat heart tissue lysate at 10 µg

Lane 3:

Rat kidney tissue lysate at 10 µg

Lane 4:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

false

Immunoprecipitation - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (AB250510)
  • IP

Supplier Data

Immunoprecipitation - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (AB250510)

This data was developed using ab181579, the same antibody clone in a different buffer formulation.

Caspase-7 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) with ab181579 at 1/120 dilution. Western blot was performed from the immunoprecipitate using ab181579 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : NIH/3T3 whole cell extract. Lane 2 : PBS instead of extract.

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed band at 35kDa band is the pro-caspase-7.

All lanes:

Immunoprecipitation - Anti-Caspase-7 antibody [EPR17029] (<a href='/en-us/products/primary-antibodies/caspase-7-antibody-epr17029-ab181579'>ab181579</a>)

Predicted band size: 34 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (AB250510)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (AB250510)

This data was developed using ab181579, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling Caspase-7 with ab181579 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm and nuclear staining on NIH/3T3 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
1. ab181579 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Please see Pubmed IDs : 22464733 and 11829465 for further information on Caspase-7 localization.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17029

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

ab250510 is the carrier-free version of ab181579.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol protease involved in different programmed cell death processes, such as apoptosis, pyroptosis or granzyme-mediated programmed cell death, by proteolytically cleaving target proteins (PubMed : 16469926, PubMed : 19168786). Has a marked preference for Asp-Glu-Val-Asp (DEVD) consensus sequences, with some plasticity for alternate non-canonical sequences (PubMed : 25231987). Its involvement in the different programmed cell death processes is probably determined by upstream proteases that activate CASP7 (PubMed : 18667412, PubMed : 35705808). Acts as an effector caspase involved in the execution phase of apoptosis : following cleavage and activation by initiator caspases (CASP8 and/or CASP9), mediates execution of apoptosis by catalyzing cleavage of proteins, such as CLSPN, PARP1, PTGES3 and YY1 (PubMed : 16469926). Compared to CASP3, acts as a minor executioner caspase and cleaves a limited set of target proteins (PubMed : 16469926). Acts as a key regulator of the inflammatory response in response to bacterial infection by catalyzing cleavage and activation of the sphingomyelin phosphodiesterase SMPD1 in the extracellular milieu, thereby promoting membrane repair (PubMed : 35705808). Regulates pyroptosis in intestinal epithelial cells : cleaved and activated by CASP1 in response to S.typhimurium infection, promoting its secretion to the extracellular milieu, where it catalyzes activation of SMPD1, generating ceramides that repair membranes and counteract the action of gasdermin-D (GSDMD) pores (PubMed : 22807671, PubMed : 35705808). Regulates granzyme-mediated programmed cell death in hepatocytes : cleaved and activated by granzyme B (GZMB) in response to bacterial infection, promoting its secretion to the extracellular milieu, where it catalyzes activation of SMPD1, generating ceramides that repair membranes and counteract the action of perforin (PRF1) pores (PubMed : 35705808). Following cleavage by CASP1 in response to inflammasome activation, catalyzes processing and inactivation of PARP1, alleviating the transcription repressor activity of PARP1 (PubMed : 22464733). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed : 30878284). Cleaves and activates sterol regulatory element binding proteins (SREBPs) (By similarity). Cleaves phospholipid scramblase proteins XKR4, XKR8 and XKR9 (PubMed : 25231987). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (By similarity).
See full target information Casp7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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Alternative Product
Primary Antibodies

AB255818

Anti-Caspase-7 antibody [EPR22839-12]

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caspase-7-antibody-epr22839-12-ab255818

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