Anti-Caspase-8 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (AB227430)

Paraffin-embedded human breast carcinoma tissue stained for Caspase 8 protein at cytoplasm with ab227430 at a 1/1000 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (AB227430)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Caspase-8 (green) using ab227430 at 1/100 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 10 minutes. Counterstained : Hoechst 33342 (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (AB227430)

Immunofluorescence analysis of 4% paraformaldehyde fixed at RT for 15 mins PG-4 cells labelling Caspase 8 protein at cytoplasm using ab227430 at a 1/100 dilution (Green). The nuclear counterstain is Hoechst 33342 (Blue).

• WB

Supplier Data

Western blot - Anti-Caspase-8 antibody (AB227430)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Caspase-8 antibody (ab227430) at 1/2000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

CASP8 (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-Caspase-8 antibody (AB227430)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Caspase-8 antibody (ab227430) at 1/1000 dilution

All lanes:

PG-4 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-Caspase-8 antibody (AB227430)

10% SDS-PAGE

All lanes:

Western blot - Anti-Caspase-8 antibody (ab227430) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

C8D30 whole cell lysate at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 55 kDa

true

• WB

Supplier Data

Western blot - Anti-Caspase-8 antibody (AB227430)

10% SDS-PAGE

All lanes:

Western blot - Anti-Caspase-8 antibody (ab227430) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 55 kDa

true

• WB

Supplier Data

Western blot - Anti-Caspase-8 antibody (AB227430)

Samples were separated by 10% SDS-PAGE. The signal was developed with ECL.

All lanes:

Western blot - Anti-Caspase-8 antibody (ab227430) at 1/1000 dilution

All lanes:

MDCK Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

true