Rabbit Polyclonal Caspase-8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CASP8.
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- International journal of molecular medicine 50:2022Time‑dependent changes in NLRP3 and Nrf2 levels in lipopolysaccharide‑induced acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRana Dhar,Ning Li,Lejun Zhang,Yajun Li,Mohammad Nasiruddin Rana,Xinwei Cao,Zhengqiang Hu,Xuefeng Wang,Xuyang Zheng,Xuanli Xu,Huifang TangPubMed 36321790
- Poultry science 101:1020342022Dietary supplementation of salidroside alleviates liver lipid metabolism disorder and inflammatory response to promote hepatocyte regeneration via PI3K/AKT/Gsk3-β pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhifu Cui,Ningning Jin,Felix Kwame Amevor,Gang Shu,Xiaxia Du,Xincheng Kang,Zifan Ning,Xun Deng,Yaofu Tian,Qing Zhu,Yan Wang,Diyan Li,Yao Zhang,Xiaoqi Wang,Xue Han,Jing Feng,Xiaoling ZhaoPubMed 35926351
- Arteriosclerosis, thrombosis, and vascular biology 42:613-6312022Caspase-8 Promotes Pulmonary Hypertension by Activating Macrophage-Associated Inflammation and IL-1β (Interleukin 1β) Production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWuwei Rong,Chenchen Liu,Xiaoming Li,Naifu Wan,Lijiang Wei,Wentong Zhu,Peiyuan Bai,Ming Li,Yangjing Ou,Fang Li,Lingxia Wang,Xuanhui Wu,Jianling Liu,Mingyan Xing,Xiaoming Zhao,Han Liu,Haibing Zhang,Ankang LyuPubMed 35387479
- Inflammation 45:1829-18472022Anti-inflammatory Effects of Novel P2X4 Receptor Antagonists, NC-2600 and NP-1815-PX, in a Murine Model of Colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVanessa D'Antongiovanni,Carolina Pellegrini,Laura Benvenuti,Matteo Fornai,Clelia Di Salvo,Gianfranco Natale,Larisa Ryskalin,Lorenzo Bertani,Elena Lucarini,Lorenzo Di Cesare Mannelli,Carla Ghelardini,Zoltan H Nemeth,György Haskó,Luca AntonioliPubMed 35338432
- Cell death discovery 7:332021Heparanase induces necroptosis of microvascular endothelial cells to promote the metastasis of hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaopeng Chen,Bin Cheng,Dafei Dai,Yuhai Wu,Zhiwen Feng,Chaogang Tong,Xiangming Wang,Jun ZhaoPubMed 33597510
- PLoS pathogens 16:e10090192020S100A4+ macrophages facilitate zika virus invasion and persistence in the seminiferous tubules via interferon-gamma mediation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Yang et. al.PubMed 33315931
- Journal of virology 94:2020Axl Deficiency Promotes the Neuroinvasion of Japanese Encephalitis Virus by Enhancing IL-1α Production from Pyroptotic Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhao-Yang Wang et. al.PubMed 32611752
- Journal of ethnopharmacology 263:1132172020Predication of the underlying mechanism of Bushenhuoxue formula acting on knee osteoarthritis via network pharmacology-based analyses combined with experimental validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui-Hui Xu et. al.PubMed 32763417
- BioMed research international 2020:80356712020Kupffer Cell-Derived TNF- Triggers the Apoptosis of Hepatic Stellate Cells through TNF-R1/Caspase 8 due to ER Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Min Wang et. al.PubMed 32802876
- Experimental and therapeutic medicine 20:409-4172020IL-17 aggravates renal injury by promoting podocyte injury in children with primary nephrotic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShubo Zhai et. al.PubMed 32537005
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CASP8. The exact immunogen used to generate this antibody is proprietary information. Database link Q14790
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (PubMed:23516580, PubMed:35338844, PubMed:35446120, PubMed:8681376, PubMed:8681377, PubMed:8962078, PubMed:9006941, PubMed:9184224). Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death (PubMed:23516580, PubMed:35338844, PubMed:35446120, PubMed:8681376, PubMed:8681377, PubMed:8962078, PubMed:9006941, PubMed:9184224). Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (PubMed:16916640, PubMed:8962078, PubMed:9006941). Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A (PubMed:8681376, PubMed:8681377). The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (PubMed:9184224). The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (PubMed:9184224). Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (PubMed:9184224). In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-324', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (PubMed:31827280, PubMed:31827281). Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-C and -D (GSDMC and GSDMD, respectively): gasdermin cleavage promotes release of the N-terminal moiety that binds to membranes and forms pores, triggering pyroptosis (PubMed:32929201, PubMed:34012073). Initiates pyroptosis following inactivation of MAP3K7/TAK1 (By similarity). Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (By similarity). May participate in the Granzyme B (GZMB) cell death pathways (PubMed:8755496). Cleaves PARP1 and PARP2 (PubMed:8681376). Independent of its protease activity, promotes cell migration following phosphorylation at Tyr-380 (PubMed:18216014, PubMed:27109099). Isoform 5. Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex. Isoform 6. Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex. Isoform 7. Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex (Probable). Acts as an inhibitor of the caspase cascade (PubMed:12010809). Isoform 8. Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Alternative names
MCH5, CASP8, Caspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, FLICE, MACH
Recommended products
Rabbit Polyclonal Caspase-8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CASP8.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CASP8. The exact immunogen used to generate this antibody is proprietary information. Database link Q14790
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Western blot - Anti-Caspase-8 antibody (ab227430)
All lanes: Western blot - Anti-Caspase-8 antibody (ab227430) at 1/500 dilution
Lane 1: WT HeLa cell extract at 30 µg
Lane 2: Caspase-8 Knockout HeLa cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa
Western blot - Anti-Caspase-8 antibody (ab227430)
10% SDS-PAGE
All lanes: Western blot - Anti-Caspase-8 antibody (ab227430) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Lane 2: C8D30 whole cell lysate at 30 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab227430)
Paraffin-embedded human colon cancer tissue stained for Caspase-8 using ab227430 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab227430)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Caspase-8 (green) using ab227430 at 1/100 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 10 minutes. Counterstained: Hoechst 33342 (blue).
Western blot - Anti-Caspase-8 antibody (ab227430)
10% SDS-PAGE
All lanes: Western blot - Anti-Caspase-8 antibody (ab227430) at 1/500 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 2: Caspase-8 knockout HeLa whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Western blot - Anti-Caspase-8 antibody (ab227430)
10% SDS-PAGE
All lanes: Western blot - Anti-Caspase-8 antibody (ab227430) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Lane 3: NCI-H929 whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Western blot - Anti-Caspase-8 antibody (ab227430)
10% SDS-PAGE
All lanes: Western blot - Anti-Caspase-8 antibody (ab227430) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Lane 2: Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
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