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AB2013

Anti-Caspase-9 antibody

4

(2 Reviews)

|

(50 Publications)

Rabbit Polyclonal Caspase-9 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 50 publications. Immunogen corresponding to Synthetic Peptide within Human CASP9 aa 1-100.

View Alternative Names

MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

5 Images
Western blot - Anti-Caspase-9 antibody (AB2013)
  • WB

Supplier Data

Western blot - Anti-Caspase-9 antibody (AB2013)

Lane 1:

Western blot - Anti-Caspase-9 antibody (ab2013) at 1 µg/mL

Lane 2:

Western blot - Anti-Caspase-9 antibody (ab2013) at 2 µg/mL

Lane 3:

Western blot - Anti-Caspase-9 antibody (ab2013) at 4 µg/mL

All lanes:

Human heart lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-Caspase-9 antibody (AB2013)
  • WB

Unknown

Western blot - Anti-Caspase-9 antibody (AB2013)

All lanes:

Western blot - Anti-Caspase-9 antibody (ab2013) at 1/1000 dilution

All lanes:

HeLa whole cell lysate

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Western blot - Anti-Caspase-9 antibody (AB2013)
  • WB

Supplier Data

Western blot - Anti-Caspase-9 antibody (AB2013)

All lanes:

Western blot - Anti-Caspase-9 antibody (ab2013) at 0.5 µg

All lanes:

Rat spleen lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-Caspase-9 antibody (AB2013)
  • WB

Supplier Data

Western blot - Anti-Caspase-9 antibody (AB2013)

Lane 1:

Western blot - Anti-Caspase-9 antibody (ab2013) at 0.5 µg/mL

Lane 2:

Western blot - Anti-Caspase-9 antibody (ab2013) at 1 µg/mL

Lane 3:

Western blot - Anti-Caspase-9 antibody (ab2013) at 2 µg/mL

All lanes:

Mouse spleen lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-Caspase-9 antibody (AB2013)
  • WB

Supplier Data

Western blot - Anti-Caspase-9 antibody (AB2013)

All lanes:

Western blot - Anti-Caspase-9 antibody (ab2013) at 1 µg/mL

Lane 1:

Daudi cell lysate at 15 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

HepG2 cell lysate at 15 µg

Lane 4:

K562 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CASP9 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P55211

Specificity

No cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase-9.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Caspase-9 Antibody is affinity chromatography purified via peptide column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758105, PubMed : 36758106).. Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.
See full target information CASP9
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Publications (50)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40937577

2025

CDC5L binds to ELAVL1 to inhibit pyroptosis in hepatocellular carcinoma through the Caspase 3/GSDME signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Liang,Zhongcheng Zhu,Yangshuo Tang,Shuhua Zhou,Moyan Xiao,Xuejun Gong,Ke Ye

Molecular medicine reports 23: PubMed33786628

2021

Costunolide attenuates oxygen‑glucose deprivation/reperfusion‑induced mitochondrial‑mediated apoptosis in PC12 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lanqing Meng,Huixia Ma,Jinni Meng,Tingting Li,Yafei Zhu,Qipeng Zhao

Cell communication and signaling : CCS 19:40 PubMed33761934

2021

Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjie Wang,Buyi Zhang,Jianli Wang,Haijian Wu,Shenbin Xu,Jianmin Zhang,Lin Wang

The Journal of international medical research 48:300060520970765 PubMed33356694

2020

Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Liu,Deqi Xiong,Rizhao Pang,Qian Deng,Nianyi Sun,Jinqi Zheng,Jiancheng Liu,Wu Xiang,Zhesi Chen,Jiachun Lu,Wenchun Wang,Anren Zhang

Experimental and therapeutic medicine 20:18 PubMed32934683

2020

Combined effect of recombinant human adenovirus p53 and curcumin in the treatment of liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Qu,Wei Lu,Ming Chen,Wei Gao,Cong Zhang,Bin Guo,Jizhi Yang

Journal of biochemical and molecular toxicology 34:e22494 PubMed32281274

2020

MiR-23b functions as an oncogenic miRNA by downregulating Mcl-1S in lung cancer cell line A549.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wang,Zhiyi Hu,Qi Guo,Litao Yang,Yuling Pang,Wei Wang

Aging 12:5152-5167 PubMed32182210

2020

Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiapeng Bao,Zhonggai Chen,Langhai Xu,Lidong Wu,Yan Xiong

Cell biology international 44:998-1008 PubMed31889370

2020

CCN5 inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shiqun Sun,Zhi Cui,Tongtong Yan,Jian Wu,Zhihui Liu

Oncology letters 19:1701-1710 PubMed32194662

2020

Role of Bcl-2 on drug resistance in breast cancer polyploidy-induced spindle poisons.

Applications

Unspecified application

Species

Unspecified reactive species

Bibo Yuan,Juan Hao,Qian Zhang,Yan Wang,Yu Zhu

American journal of physiology. Endocrinology and 318:E723-E735 PubMed31910027

2020

Downregulation of microRNA-873 attenuates insulin resistance and myocardial injury in rats with gestational diabetes mellitus by upregulating IGFBP2.

Applications

Unspecified application

Species

Unspecified reactive species

Na Han,Hai-Yan Fang,Jie-Xuan Jiang,Qian Xu
View all publications
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