Rabbit Polyclonal Caspase-9 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human CASP9 aa 1-100.
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- Molecular medicine reports 23:2021Costunolide attenuates oxygen‑glucose deprivation/reperfusion‑induced mitochondrial‑mediated apoptosis in PC12 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLanqing Meng,Huixia Ma,Jinni Meng,Tingting Li,Yafei Zhu,Qipeng ZhaoPubMed 33786628
- Cell communication and signaling : CCS 19:402021Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongjie Wang,Buyi Zhang,Jianli Wang,Haijian Wu,Shenbin Xu,Jianmin Zhang,Lin WangPubMed 33761934
- The Journal of international medical research 48:3000605209707652020Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Liu,Deqi Xiong,Rizhao Pang,Qian Deng,Nianyi Sun,Jinqi Zheng,Jiancheng Liu,Wu Xiang,Zhesi Chen,Jiachun Lu,Wenchun Wang,Anren ZhangPubMed 33356694
- Journal of biochemical and molecular toxicology 34:e224942020MiR-23b functions as an oncogenic miRNA by downregulating Mcl-1S in lung cancer cell line A549.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Wang,Zhiyi Hu,Qi Guo,Litao Yang,Yuling Pang,Wei WangPubMed 32281274
- Aging 12:5152-51672020Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiapeng Bao et. al.PubMed 32182210
- Cell biology international 44:998-10082020CCN5 inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiqun Sun,Zhi Cui,Tongtong Yan,Jian Wu,Zhihui LiuPubMed 31889370
- Oncology letters 19:1701-17102020Role of Bcl-2 on drug resistance in breast cancer polyploidy-induced spindle poisons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBibo Yuan et. al.PubMed 32194662
- Oncology letters 19:255-2602020Oridonin induces apoptosis in HGC-27 cells by activating the JNK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong-Lin Ren,Roshan Ara Ghoorun,Xiao-Hua Wu,Hong-Lei Chen,Qian Zhou,Xiao-Bin WuPubMed 31897137
- Cell cycle (Georgetown, Tex.) 19:207-2172019Swainsonine protects H9c2 cells against lipopolysaccharide-induced apoptosis and inflammatory injury via down-regulating miR-429.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Lv,Su Zhang,Xiaohong HaoPubMed 31876239
- Cell cycle (Georgetown, Tex.) 18:2026-20392019Inhibitory effect of AQP1 silencing on adhesion and angiogenesis in ectopic endometrial cells of mice with endometriosis through activating the Wnt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang Shu et. al.PubMed 31251110
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CASP9 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P55211
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Species Rat | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758105, PubMed:36758106). Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.
Alternative names
MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6
Recommended products
Rabbit Polyclonal Caspase-9 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human CASP9 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CASP9 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P55211
- Purification technique
- Affinity purification Immunogen
- Specificity
No cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase-9.
- Concentration
- Purification notes
Caspase-9 Antibody is affinity chromatography purified via peptide column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Anti-Caspase-9 antibody (ab2013)
All lanes: Western blot - Anti-Caspase-9 antibody (ab2013) at 1 µg/mL
Lane 1: Daudi cell lysate at 15 µg
Lane 2: HeLa cell lysate at 15 µg
Lane 3: HepG2 cell lysate at 15 µg
Lane 4: K562 cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa
Western blot - Anti-Caspase-9 antibody (ab2013)
Lane 1: Western blot - Anti-Caspase-9 antibody (ab2013) at 1 µg/mL
Lane 2: Western blot - Anti-Caspase-9 antibody (ab2013) at 2 µg/mL
Lane 3: Western blot - Anti-Caspase-9 antibody (ab2013) at 4 µg/mL
All lanes: Human heart lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa
Western blot - Anti-Caspase-9 antibody (ab2013)
Lane 1: Western blot - Anti-Caspase-9 antibody (ab2013) at 0.5 µg/mL
Lane 2: Western blot - Anti-Caspase-9 antibody (ab2013) at 1 µg/mL
Lane 3: Western blot - Anti-Caspase-9 antibody (ab2013) at 2 µg/mL
All lanes: Mouse spleen lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa
Western blot - Anti-Caspase-9 antibody (ab2013)
All lanes: Western blot - Anti-Caspase-9 antibody (ab2013) at 0.5 µg
All lanes: Rat spleen lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa
Western blot - Anti-Caspase-9 antibody (ab2013)
All lanes: Western blot - Anti-Caspase-9 antibody (ab2013) at 1/1000 dilution
All lanes: HeLa whole cell lysate
Predicted band size: 46 kDa
Observed band size: 46 kDa
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