AB25758

Anti-Caspase-9 antibody

(1 Review)

(74 Publications)

Rabbit Polyclonal Caspase-9 antibody. Suitable for WB and reacts with Mouse samples. Cited in 74 publications. Immunogen corresponding to Synthetic Peptide within Human CASP9.

View Alternative Names

MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

1 Images

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Western blot - Anti-Caspase-9 antibody (AB25758)

The antibody detects 45 kDa procaspase 9, 35/37 kDa cleaved immediate fragment and the 17-25 kDa fully cleaved caspase 9.

All lanes:

Western blot - Anti-Caspase-9 antibody (ab25758)

All lanes:

mouse intestine tissue lysate

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human CASP9. The exact immunogen used to generate this antibody is proprietary information.

P55211

Specificity

ab25758 detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9. The antibody does not detect any other caspases.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3136 Caspase-9 Antibody.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758105, PubMed : 36758106).. Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.

See full target information CASP9

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Publications (74)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38542166

2024

Beneficial Effects of Fibroblast Growth Factor-1 on Retinal Pigment Epithelial Cells Exposed to High Glucose-Induced Damage: Alleviation of Oxidative Stress, Endoplasmic Reticulum Stress, and Enhancement of Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Wei Huang,Chung-May Yang,Chang-Hao Yang

Immunity, inflammation and disease 12:e1135 PubMed38270316

2024

Sirtuin 3 is required for the dexmedetomidine-mediated alleviation of inflammation and oxidative stress in nephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Lu,Xinlong Li,Jie Wu

Heliyon 9:e21879 PubMed38076203

2023

Taurine alleviates high-fat-high-glucose-induced pancreatic islet β-cell oxidative stress and apoptosis in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Dongdong Zhao,Deqi Yin,Xinxin Wang,Yanxi Li,Miao He,Jianmin Hu,Lin Shumei,Jiancheng Yang

Oncology research 30:289-300 PubMed37303493

2023

LSM12 facilitates the progression of colorectal cancer by activating the WNT/CTNNB1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhuang,Chunlan Ning,Pengfei Liu,Yanpeng Zhao,Yue Li,Zhenchi Ma,Luling Shan,Yingzhe Piao,Peng Zhao,Xun Jin

Experimental & molecular medicine 54:1991-2006 PubMed36380019

2022

Lnc Tmem235 promotes repair of early steroid-induced osteonecrosis of the femoral head by inhibiting hypoxia-induced apoptosis of BMSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Zhang,Wuxun Peng,Tao Wang,Jian Zhang,Wentao Dong,Chuan Wang,Zhihong Xie,Hong Luo,Gang Liu

Cell journal 24:657-664 PubMed36377215

2022

Influences The Occurrence and Progression of Liver Cancer by Inhibiting Mitochondrial Apoptosis Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Hua Sun,Yang-Long Li,Hao Jiang,Xiang-Hua Yin,Xing-Lin Jin

Drug design, development and therapy 16:2545-2557 PubMed35959422

2022

The Flavagline Compound 1-(2-(dimethylamino)acetyl)-Rocaglaol Induces Apoptosis in K562 Cells by Regulating the PI3K/Akt/mTOR, JAK2/STAT3, and MAPK Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xinmei Yang,Xijun Wu,Xiaosen Wu,Lei Huang,Jingrui Song,Chunmao Yuan,Zhixu He,Yanmei Li

Journal of immunology research 2022:4903333 PubMed35879950

2022

Flavokawain B Weakens Gastric Cancer Progression via the TGF-1/SMAD4 Pathway and Attenuates M2 Macrophage Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yongzhao Zhu,Weining Fan,Yuanzhen Wang,Huan Ding,Shaoqi Yang,Fang He

Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer 41:47-59 PubMed35695651

2022

Exosomal circRNA_104948 Enhances the Progression of Glioma by Regulating miR-29b-3p and DNMT3B/MTSS1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shoudan Zhang,Ning Guan,Xin Mao,Jingwen Cui,Xin Sui,Wenshi Guo

Translational neuroscience 13:104-115 PubMed35734308

2022

Remimazolam induced cognitive dysfunction in mice via glutamate excitotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Hua Zhou,Cheng-Cheng Zhang,Ling Wang,Shan-Liang Jin

View all publications

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