Rabbit Recombinant Monoclonal Caspase-9 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 15 publications.
Images
![Western blot - Anti-Caspase-9 antibody [E84] (AB32068), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-e84-ab32068--western-blot-img53621.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-9 antibody [E84] (AB32068)")
![Western blot - Anti-Caspase-9 antibody [E84] (AB32068), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-e84-ab32068--western-blot-img60658.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-9 antibody [E84] (AB32068)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] (AB32068), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-e84-ab32068--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115560.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] (AB32068)")
![Western blot - Anti-Caspase-9 antibody [E84] (AB32068), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-e84-ab32068--western-blot-img150704.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-9 antibody [E84] (AB32068)")
Publications
Filter by
- Oncology reports 48:2022induces growth inhibition and autophagy‑related apoptosis by regulating the AMPK and Akt/mTOR/p70S6K signaling pathways in cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeixiao An,Yuxi Zhang,Honglin Lai,Yangyang Zhang,Hongmei Zhang,Ge Zhao,Minghua Liu,Yang Li,Xiukun Lin,Shousong CaoPubMed 35730618
- Artificial cells, nanomedicine, and biotechnology 47:1758-17652019Salidroside mitigates hydrogen peroxide-induced injury by enhancement of microRNA-27a in human trabecular meshwork cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Zhao,Xiujuan Du,Meng Wang,Peiyao Yang,Juanmei ZhangPubMed 31062616
- Molecular and cellular biochemistry 456:63-722019Lidocaine alleviates cytotoxicity-resistance in lung cancer A549/DDP cells via down-regulation of miR-21.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Yang et. al.PubMed 30644017
- Experimental and molecular pathology 106:109-1152018Lycium barbarum polysaccharides alleviate hydrogen peroxide-induced injury by up-regulation of miR-4295 in human trabecular meshwork cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxia Liu et. al.PubMed 30594603
- Molecules and cells 41:401-4122018Oxymatrine Causes Hepatotoxicity by Promoting the Phosphorylation of JNK and Induction of Endoplasmic Reticulum Stress Mediated by ROS in LO2 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Li Gu,Zhe-Lun Shen,Yang-Lei Li,Yi-Qi Bao,Hong LuPubMed 29754474
- Oncology research 27:139-1462018Liquiritigenin Inhibits Colorectal Cancer Proliferation, Invasion, and Epithelial-to-Mesenchymal Transition by Decreasing Expression of Runt-Related Transcription Factor 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan-Chun Meng,Jun-Kai LinPubMed 29471888
- Cellular physiology and biochemistry : internation 45:832-8432018Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaitao Sun et. al.PubMed 29414810
- Experimental and therapeutic medicine 14:5057-50622017Sirt1 regulates apoptosis and extracellular matrix degradation in resveratrol-treated osteoarthritis chondrocytes via the Wnt/β-catenin signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuan Liu et. al.PubMed 29201214
- International journal of clinical and experimental 10:9293-93012017MicroRNA-139-5p alleviates UVB-induced injuries by regulating TLR4 in si-IL-6-treated keratinocyte cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Yu et. al.PubMed 31966801
- International journal of clinical and experimental 10:8211-82212017MicroRNA-641 inhibits lung cancer cells proliferation, metastasis but promotes apoptosis in cells by targeting PDCD4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Zhou et. al.PubMed 31966672
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
Associated Products
Select an associated product type
14 products for Alternative Product
Target data
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758105, PubMed:36758106). Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.
Alternative names
MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6
Recommended products
Rabbit Recombinant Monoclonal Caspase-9 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 15 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- E84
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Caspase-9 antibody [E84] (ab32068), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-e84-ab32068--western-blot-img53621.jpg "Western blot - Anti-Caspase-9 antibody [E84] (ab32068)")
Western blot - Anti-Caspase-9 antibody [E84] (ab32068)
Lanes:
Lane 1: Wild-type HAP1 whole cell lysate (40 μg).
Lane 2: CASP9 knockout HAP1 whole cell lysate (40μg).
Lane 3: HeLa whole cell lysate (40 μg).Lanes 1 - 3 Merged signal (red and green). Green - ab32068 observed at 45 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab32068 was shown to specifically recognize CASP9 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CASP9 knockout samples were examined. Wild-type and CASP9 knockout samples were subjected to SDS-PAGE. ab32068 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Caspase-9 antibody [E84] (ab32068)
Predicted band size: 46 kDa
![Western blot - Anti-Caspase-9 antibody [E84] (ab32068), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-e84-ab32068--western-blot-img60658.png "Western blot - Anti-Caspase-9 antibody [E84] (ab32068)")
Western blot - Anti-Caspase-9 antibody [E84] (ab32068)
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Caspase-9 antibody [E84] (ab32068) at 1/1000 dilution
Lane 1: Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: HeLa treated with staurosporine 1uM for 4 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 35 kDa, 37 kDa, 46 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] (ab32068), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-e84-ab32068--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115560.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] (ab32068)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] (ab32068)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb (ab32068).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Caspase-9 antibody [E84] (ab32068), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-e84-ab32068--western-blot-img150704.jpg "Western blot - Anti-Caspase-9 antibody [E84] (ab32068)")
Western blot - Anti-Caspase-9 antibody [E84] (ab32068)
All lanes: Western blot - Anti-Caspase-9 antibody [E84] (ab32068) at 1/2000 dilution
All lanes: Jurkat cell lysate at 10 µg
Predicted band size: 46 kDa
Observed band size: 46 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com