Rabbit Recombinant Monoclonal CASP9 antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 44 publications.
Images
![Western blot - Anti-Caspase-9 antibody [EPR18868] (AB184786), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-ab184786--western-blot-img10798.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-9 antibody [EPR18868] (AB184786)")
![Western blot - Anti-Caspase-9 antibody [EPR18868] (AB184786), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-ab184786--western-blot-img14706.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-9 antibody [EPR18868] (AB184786)")
![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (AB184786), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-ab184786--immunoprecipitation-img333.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (AB184786)")
![Western blot - Anti-Caspase-9 antibody [EPR18868] (AB184786), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-ab184786--western-blot-img20251.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-9 antibody [EPR18868] (AB184786)")
![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (AB184786), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-ab184786--immunoprecipitation-img466.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (AB184786)")
Publications
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- Aging 15:12264-122742023Influences and mechanism of erythropoietin on the cognitive function of vascular dementia rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhipeng Tang,Xiuqin Li,Nan Yin,Ming Zhao,Qingchuan Hu,Peiyuan LvPubMed 37934569
- Frontiers in cardiovascular medicine 10:11814732023Optimal treatment for post-MI heart failure in rats: dapagliflozin first, adding sacubitril-valsartan 2 weeks later.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenqi Tao,Xiaoyu Yang,Qing Zhang,Shuli Bi,Zhuhua YaoPubMed 37383701
- Integrative medicine research 12:1009462023Anti-oxidative-initiated cognitive impairment amelioration in Alzheimer's disease model rats through preventive transcutaneous electrical acupoint stimulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng Weng,Qing Wang,Ran Ye,Yamei Bai,Hui Yang,Guihua Xu,Qiuqin WangPubMed 37187681
- Frontiers in aging neuroscience 14:9906792022Sevoflurane exposure causes neuronal apoptosis and cognitive dysfunction by inducing ER stress activation of the inositol 1, 4, 5-trisphosphate receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Zhang,Yanan Li,Xupeng Wang,Chunping Yin,Qi Zhou,Junfei Guo,Juan Zhao,Xiaohui Xian,Zhiyong Hou,Qiujun WangPubMed 36337694
- Nature protocols 17:2188-22152022Scalable tissue labeling and clearing of intact human organs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongcheng Mai,Zhouyi Rong,Shan Zhao,Ruiyao Cai,Hanno Steinke,Ingo Bechmann,Ali ErtürkPubMed 35859136
- Experimental and therapeutic medicine 24:5332022Esculetin has therapeutic potential via the proapoptotic signaling pathway in A253 human submandibular salivary gland tumor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSu-Bin Park,Woo Kwon Jung,Hyung Rae Kim,Hwa-Young Yu,Yong Hwan Kim,Junghyun KimPubMed 35837055
- Oxidative medicine and cellular longevity 2022:34582832022Mammalian Sterile 20-Like Kinase 1 Mediates Neuropathic Pain Associated with Its Effects on Regulating Mitophagy in Schwann Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi Huang,Pei-Yao Xiao,Jing-Yan Chen,Qing Zeng,Bei-Xu Huang,Jian Yu,Song-Jie LiaoPubMed 35656021
- Frontiers in physiology 13:8113692022Effect of 32-Weeks High-Intensity Interval Training and Resistance Training on Delaying Sarcopenia: Focus on Endogenous Apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Su,Tianhao Wen,Dongsen Liu,Jia Shao,Lei Zhao,Qi GaoPubMed 35574455
- Biochemistry research international 2022:99482292022miR-21 Regulates Immune Balance Mediated by Th17/Treg in Peripheral Blood of Septic Rats during the Early Phase through Apoptosis Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Liu,Qi ZouPubMed 35528843
- International journal of immunopathology and pharmacology 36:39463202210860792022Evodiamine suppresses the progression of non-small cell lung carcinoma via endoplasmic reticulum stress-mediated apoptosis pathway and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuting Li,Yuming Wang,Xiaoqun Wang,Lulu Jin,Lu Yang,Jinli Zhu,Hongwu Wang,Fang Zheng,Huantian Cui,Xiaojiang Li,Yingjie JiaPubMed 35388733
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species Rat | Dilution info 1/30 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Associated Products
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Target data
Recommended products
Rabbit Recombinant Monoclonal CASP9 antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 44 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR18868
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-ab184786--western-blot-img10798.jpg "Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)")
Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)
Blocking/Dilution buffer: 5% NFDM/TBST.
The p39 and p37 subunits were inhibited when the caspase activity was blocked by the caspase inhibitor, Z-VAD-FMK.
All lanes: Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1: Untreated C6 (Rat glial tumor cells) whole cell lysate at 20 µg
Lane 2: C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 0.5 hour at 20 µg
Lane 3: C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 1 hour at 20 µg
Lane 4: C6 whole cell lysate treated with 1 µm staurosporine for 4 hours at 20 µg
Lane 5: C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 1 hour, then treated with 1 µM staurosporine for 4 hours at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 37 kDa, 39 kDa, 50 kDa
Exposure time: 10s
![Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-ab184786--western-blot-img14706.jpg "Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)")
Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1: Untreated NIH/3T3 (Mouse embyro fibroblast cell line) whole cell lysate at 20 µg
Lane 2: NIH/3T3 whole cell lysate treated with 1 µM staurosporine for 4 hours at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 37 kDa, 39 kDa, 50 kDa
Exposure time: 15s
![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-ab184786--immunoprecipitation-img333.jpg "Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786)")
Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786)
Caspase 9 was immunoprecipitated from 1mg of NIH/3T3 whole cell lysate (Mouse embyro fibroblast cells) treated with 1μM staurosporine for 4h with ab184786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184786 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h,10ug (Input).
Lane 2: ab184786 IP in NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184786 in NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.The product has better affinity to procaspase-9. The cleaved form p37 could not be observed even with 3 minutes exposure time.
All lanes: Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786)
Predicted band size: 46 kDa
![Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-ab184786--western-blot-img20251.jpg "Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)")
Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse spleen lysate at 10 µg
Lane 4: Rat brain lysate at 10 µg
Lane 5: Rat heart lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 37 kDa, 39 kDa, 50 kDa
Exposure time: 20s
![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-ab184786--immunoprecipitation-img466.jpg "Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786)")
Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786)
Caspase 9 was immunoprecipitated from 1mg of C6 whole cell lysate (Rat glial tumor cells) treated with 1μM staurosporine for 4h with ab184786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184786 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: C6 whole cell lysate treated with 1μM staurosporine for 4h, 10ug (Input).
Lane 2: ab184786 IP in C6 whole cell lysate treated with 1μM staurosporine for 4h.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184786 in C6 whole cell lysate treated with 1μM staurosporine for 4h.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.The product has better affinity to procaspase-9. The cleaved form p37 could be observed with longer exposure time.
All lanes: Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786)
Predicted band size: 46 kDa
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