Rabbit Recombinant Monoclonal CASP9 antibody. Carrier free. Suitable for IP, WB and reacts with Rat, Mouse samples.
Images
![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] - BSA and Azide free (AB240287), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-bsa-and-azide-free-ab240287--immunoprecipitation-img333.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] - BSA and Azide free (AB240287)")
![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] - BSA and Azide free (AB240287), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-bsa-and-azide-free-ab240287--immunoprecipitation-img466.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] - BSA and Azide free (AB240287)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Mouse | Predicted | Expected |
| Rat | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
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Rabbit Recombinant Monoclonal CASP9 antibody. Carrier free. Suitable for IP, WB and reacts with Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18868
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240287 is the carrier-free version of Anti-Caspase-9 antibody [EPR18868] ab184786.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] - BSA and Azide free (ab240287), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-bsa-and-azide-free-ab240287--immunoprecipitation-img333.jpg "Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] - BSA and Azide free (ab240287)")
Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] - BSA and Azide free (ab240287)
Caspase 9 was immunoprecipitated from 1mg of NIH/3T3 whole cell lysate (Mouse embyro fibroblast cells) treated with 1μM staurosporine for 4h with Anti-Caspase-9 antibody [EPR18868] ab184786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Caspase-9 antibody [EPR18868] ab184786 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h,10ug (Input).
Lane 2: Anti-Caspase-9 antibody [EPR18868] ab184786 IP in NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Caspase-9 antibody [EPR18868] ab184786 in NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.The product has better affinity to procaspase-9. The cleaved form p37 could not be observed even with 3 minutes exposure time.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caspase-9 antibody [EPR18868] ab184786).
All lanes: Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (Anti-Caspase-9 antibody [EPR18868] ab184786)
Predicted band size: 46 kDa
![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] - BSA and Azide free (ab240287), expandable thumbnail](https://content.abcam.com/products/images/caspase-9-antibody-epr18868-bsa-and-azide-free-ab240287--immunoprecipitation-img466.jpg "Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] - BSA and Azide free (ab240287)")
Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] - BSA and Azide free (ab240287)
Caspase 9 was immunoprecipitated from 1mg of C6 whole cell lysate (Rat glial tumor cells) treated with 1μM staurosporine for 4h with Anti-Caspase-9 antibody [EPR18868] ab184786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Caspase-9 antibody [EPR18868] ab184786 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: C6 whole cell lysate treated with 1μM staurosporine for 4h, 10ug (Input).
Lane 2: Anti-Caspase-9 antibody [EPR18868] ab184786 IP in C6 whole cell lysate treated with 1μM staurosporine for 4h.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Caspase-9 antibody [EPR18868] ab184786 in C6 whole cell lysate treated with 1μM staurosporine for 4h.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.The product has better affinity to procaspase-9. The cleaved form p37 could be observed with longer exposure time.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caspase-9 antibody [EPR18868] ab184786).
All lanes: Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (Anti-Caspase-9 antibody [EPR18868] ab184786)
Predicted band size: 46 kDa
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