Rabbit Polyclonal Caspase-9 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CASP9 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Neoplasma 68:362-3742020LncRNA MEG3 promotes endoplasmic reticulum stress and suppresses proliferation and invasion of colorectal carcinoma cells through the MEG3/miR-103a-3p/PDHB ceRNA pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoqin Wang,Qiuwen Ye,Shujuan Ning,Zhuangqing Yang,Yunlan Chen,Lijuan Zhang,Youguang Huang,Fujia Xie,Xi Cheng,Junlin Chi,Yi Lei,Rong Guo,Jiangqiong HanPubMed 33118833
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CASP9 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P55211
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Expected | Expected | Tested |
| Dog | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
Target data
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758105, PubMed:36758106). Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.
Alternative names
MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6
Recommended products
Rabbit Polyclonal Caspase-9 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CASP9 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CASP9 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P55211
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Caspase-9 p35 antibody (ab210611)
All lanes: Western blot - Anti-Caspase-9 p35 antibody (ab210611) at 1/300 dilution
All lanes: Raji lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution
Predicted band size: 46 kDa
Flow Cytometry (Intracellular) - Anti-Caspase-9 p35 antibody (ab210611)
Intracellular flow cytometric analysis of RSC96cells labeling Caspase-9 p35with ab210611 at 1/100 dilution for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 p35 antibody (ab210611)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Caspase-9 p35 with ab210611 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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