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AB61789

Anti-Caspase-9 (phospho S196) antibody

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(2 Publications)

Rabbit Polyclonal Caspase-9 phospho S196 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CASP9 phospho S196 aa 150-250.

View Alternative Names

MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

1 Images
Western blot - Anti-Caspase-9 (phospho S196) antibody (AB61789)
  • WB

Unknown

Western blot - Anti-Caspase-9 (phospho S196) antibody (AB61789)

All lanes:

Western blot - Anti-Caspase-9 (phospho S196) antibody (ab61789) at 1/500 dilution

Lane 1:

Extracts from 293 cells treated with Calyculin (50nM, 30mins)

Lane 2:

Extracts from 293 cells treated with Calyculin (50nM, 30mins) and with the immunising phosphopeptide

Predicted band size: 46 kDa

Observed band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CASP9 phospho S196 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

P55211

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758105, PubMed : 36758106).. Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.
See full target information CASP9 phospho S196
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 18:902-910 PubMed29767235

2018

Inhibition of microRNA‑16 protects mesenchymal stem cells against apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Rui,Shaohong Fang,Yongchen Wang,Bo Lv,Bo Yu,Shufeng Li

The Journal of biological chemistry 286:16606-14 PubMed21454583

2011

miRNA-205 suppresses melanoma cell proliferation and induces senescence via regulation of E2F1 protein.

Applications

WB

Species

Human

Altaf A Dar,Shahana Majid,David de Semir,Mehdi Nosrati,Vladimir Bezrookove,Mohammed Kashani-Sabet
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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