Rabbit Polyclonal Caspase-9 phospho S196 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CASP9 phospho S196 aa 150-250.
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Publications
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- Molecular medicine reports 18:902-9102018Inhibition of microRNA‑16 protects mesenchymal stem cells against apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiang Rui et. al.PubMed 29767235
- The Journal of biological chemistry 286:16606-142011miRNA-205 suppresses melanoma cell proliferation and induces senescence via regulation of E2F1 protein.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CASP9 phospho S196 aa 150-250. The exact immunogen used to generate this antibody is proprietary information. Database link P55211
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
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Target data
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758105, PubMed:36758106). Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.
Alternative names
MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6
Recommended products
Rabbit Polyclonal Caspase-9 phospho S196 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CASP9 phospho S196 aa 150-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CASP9 phospho S196 aa 150-250. The exact immunogen used to generate this antibody is proprietary information. Database link P55211
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Caspase-9 (phospho S196) antibody (ab61789)
All lanes: Western blot - Anti-Caspase-9 (phospho S196) antibody (ab61789) at 1/500 dilution
Lane 1: Extracts from 293 cells treated with Calyculin (50nM, 30mins)
Lane 2: Extracts from 293 cells treated with Calyculin (50nM, 30mins) and with the immunising phosphopeptide
Predicted band size: 46 kDa
Observed band size: 46 kDa
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