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AB79202

Anti-Caspase-9 (phospho Y153) antibody

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(2 Publications)

Rabbit Polyclonal Caspase-9 phospho Y153 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CASP9 phospho Y153.

View Alternative Names

MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

3 Images
Western blot - Anti-Caspase-9 (phospho Y153) antibody (AB79202)
  • WB

Unknown

Western blot - Anti-Caspase-9 (phospho Y153) antibody (AB79202)

All lanes:

Western blot - Anti-Caspase-9 (phospho Y153) antibody (ab79202) at 1/500 dilution

Lane 1:

COLO205 cell extracts at 30 µg

Lane 2:

COLO205 cell extracts at 30 µg with immunizing peptide

Predicted band size: 46 kDa

Observed band size: 40 kDa,46 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 (phospho Y153) antibody (AB79202)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 (phospho Y153) antibody (AB79202)

Left panel : immunofluorescence analysis of HeLa cells using 1/500 ab89202. Right panel : same, after pre-incubation with the immunizing phosphopeptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 (phospho Y153) antibody (AB79202)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 (phospho Y153) antibody (AB79202)

Left panel : immunohistochemistry analysis of paraffin-embedded Human thymus tissue using 1/50 ab79202. Right panel : same, after pre-incubation with the immunizing phosphopeptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human CASP9 phospho Y153. The exact immunogen used to generate this antibody is proprietary information.

P55211

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758105, PubMed : 36758106).. Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.
See full target information CASP9 phospho Y153
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 11: PubMed36552682

2022

Sidestream Smoke Extracts from Harm-Reduction and Conventional Camel Cigarettes Inhibit Osteogenic Differentiation via Oxidative Stress and Differential Activation of intrinsic Apoptotic Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole R L Sparks,Lauren M Walker,Steven R Sera,Joseph V Madrid,Michael Hanna,Edward C Dominguez,Nicole I Zur Nieden

Journal of cellular biochemistry 120:836-847 PubMed30125989

2018

Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a.

Applications

Unspecified application

Species

Unspecified reactive species

Weijun Gong,Shuyan Qie,Peiling Huang,Jianing Xi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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