AB153856

Anti-Caspr2/CNTNAP2 antibody

Name: Anti-Caspr2/CNTNAP2 antibody (ab153856) | Abcam
Brand: Abcam Limited
SKU: AB153856
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Caspr2/CNTNAP2 antibody. Suitable for WB, ICC/IF, IHC-Fr and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CNTNAP2 aa 250-650.

View Alternative Names

CASPR2, KIAA0868, CNTNAP2, Contactin-associated protein-like 2, Cell recognition molecule Caspr2

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (AB153856)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (AB153856)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Caspr2/CNTNAP2 with ab153856 at 1/200 dilution. Lower panel is merged with DNA probe.

Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (AB153856)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (AB153856)

Frozen section of embryonic mouse brain (mE18.5) staining Caspr2/CNTNAP2 proteins in embryonic mouse brain by immunohistochemical analysis.
Red : ab153856 diluted at 1 : 250.
Blue : DAPI.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (AB153856)

Frozen sectioned E13.5 Rat brain staining Caspr2/CNTNAP2 protein expression by immunohistochemical analysis.
Green : ab153856 diluted at 1 : 250.
Red : beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (AB153856)

DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min staining Caspr2/CNTNAP2 protein by immunofluorescent analysis (1 : 500) (green).
Counter stained using beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1 : 500 (red) and DAPI (blue).

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Western blot - Anti-Caspr2/CNTNAP2 antibody (AB153856)

5% SDS PAGE

All lanes:

Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856) at 1/5000 dilution

All lanes:

Molt-4 whole cell lysate at 30 µg

Predicted band size: 148 kDa

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Western blot - Anti-Caspr2/CNTNAP2 antibody (AB153856)

5% SDS PAGE

All lanes:

Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856) at 1/10000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 148 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-Fr, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CNTNAP2 aa 250-650. The exact immunogen used to generate this antibody is proprietary information.

Q9UHC6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caspr2 also known as CNTNAP2 is a member of the neurexin family with a molecular mass of approximately 133 kDa. This protein extensively expresses in the nervous system and particularly locates on the axonal membrane. Caspr2 functions as a cell adhesion molecule playing a role in facilitating interactions between neurons and glial cells. Additionally Caspr2 participates in the clustering of ion channels at the nodes of Ranvier which is important for rapid action potential propagation along myelinated axons.

Biological function summary

This protein is important in maintaining the integrity of the neuronal membrane. Caspr2 works as part of a complex associating with contactin proteins to stabilize neuronal networks. It is involved in synaptic plasticity and potentially influences neuronal connectivity and communication. Through its adhesive functions Caspr2 contributes to structural frameworks that ensure proper signal transmission in the neural circuits.

Pathways

Caspr2 participates in pathways responsible for neuronal excitability and membrane potential regulation. It plays an important role within the voltage-gated potassium channel complex. Caspr2 also connects to proteins like TAG-1 forming a vital part of the complex involved in the assembly of potassium channels and ensuring efficient nerve impulse conduction. Its interaction within these pathways impacts synaptic function and cognitive processes by modulating neuronal excitability.

Caspr2 has links to epilepsy and autism spectrum disorders where disruptions in its function can lead to pathogenic outcomes. Alterations in Caspr2 expression or structure may result in impaired neuron-glial interactions contributing to neurological symptoms. It relates to contactin-associated proteins in these contexts potentially affecting the development and maintenance of neuronal structures. Understanding Caspr2’s involvement in these diseases helps in developing therapeutic strategies and antibodies such as anti-Caspr2 antibodies to modulate its activity and effects.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for gap junction formation (Probable). Required, with CNTNAP1, for radial and longitudinal organization of myelinated axons. Plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Demarcates the juxtaparanodal region of the axo-glial junction.

See full target information CNTNAP2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 58:102544 PubMed36427397

2022

CPT1A promotes anoikis resistance in esophageal squamous cell carcinoma via redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Tian,Yunxin Lu,Jinfei Lin,Miao Chen,Huijuan Qiu,Wancui Zhu,Haohui Sun,Jinsheng Huang,Han Yang,Wuguo Deng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Caspr2/CNTNAP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (AB153856)

Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (AB153856)

Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (AB153856)

Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (AB153856)

Western blot - Anti-Caspr2/CNTNAP2 antibody (AB153856)

Western blot - Anti-Caspr2/CNTNAP2 antibody (AB153856)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

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Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise