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Rabbit Recombinant Monoclonal CASPR3 antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse samples.

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Images

Western blot - Anti-CASPR3 antibody [EPR22727-102] (AB252413), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413), expandable thumbnail
  • Western blot - Anti-CASPR3 antibody [EPR22727-102] (AB252413), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBIHC-Fr
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species
Human
Dilution info
-
Notes

-

Target data

Alternative names

CASPR3, KIAA1714, CNTNAP3, Contactin-associated protein-like 3, Cell recognition molecule Caspr3

Recommended products

Rabbit Recombinant Monoclonal CASPR3 antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22727-102
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CASPR3 also known as contactin-associated protein-like 3 is a member of the neurexin superfamily with an approximate mass of 142 kDa. This protein typically localizes at the plasma membrane within neural tissues. CASPR3 plays a role in cell adhesion which is important for synaptic function and neural development. Expression of CASPR3 is most regularly observed in the central nervous system where it contributes to the organization of the neuronal network.

Biological function summary

CASPR3 assists in axonal guidance by interacting with other proteins to form complexes necessary for neural communication. It is part of the axon-associated contactin–Caspr complex which is essential for the formation of paranodal junctions in neurons. These junctions ensure the proper functioning of myelinated axons through the coordination of neuronal signaling affecting processes like learning and memory.

Pathways

CASPR3 interacts with molecular pathways that are integral to the maintenance of neuronal architecture and signal transmission. It is involved in the neuregulin signaling pathway which affects neural glial communication. CASPR3 works alongside proteins such as CNTN1 (Contactin 1) that further aid in neural connectivity and axonal integrity. These interactions ensure the rapid transmission of nerve impulses which is important for efficient neural processing.

Associated diseases and disorders

CASPR3 has associations with neurological conditions such as autism spectrum disorders and schizophrenia. Alterations or malfunctions in CASPR3 can disrupt synaptic adhesion and neural processing leading to symptomatic manifestations of these conditions. CASPR3 links with other proteins related to these diseases such as CNTN1 suggesting a broader impact on the neural network that underlies cognitive and behavioral health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-CASPR3 antibody [EPR22727-102] (ab252413), expandable thumbnail

    Western blot - Anti-CASPR3 antibody [EPR22727-102] (ab252413)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot was developed using a higher sensitivity ECL substrate.

    The lysates were kindly provided by Prof. Zilong Qiu, Institute of Neuroscience (ION), CAS.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-CASPR3 antibody [EPR22727-102] (ab252413) at 1/1000 dilution

    Lane 1: Wild-type mouse adult prefrontal cortex tissue lysate at 20 µg

    Lane 2: CNTNAP3 knock out mouse adult prefrontal cortex tissue lysate at 20 µg

    Lane 3: Wild-type mouse adult thalamus tissue lysate at 20 µg

    Lane 4: CNTNAP3 knock out mouse adult thalamus tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 141 kDa

    Observed band size: 150 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (ab252413), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (ab252413)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Caspr3/CNTNAP3 with ab252413 at 1/2000 (0.274 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Postive staining on mouse cerebrum (PMID: 26807827). The section was incubated with ab252413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-CASPR3 antibody [EPR22727-102] (ab252413), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CASPR3 antibody [EPR22727-102] (ab252413)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Caspr3/CNTNAP3 with ab252413 at 1/50 (10.94 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-CASPR3 antibody [EPR22727-102] (ab252413), expandable thumbnail

    Western blot - Anti-CASPR3 antibody [EPR22727-102] (ab252413)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 92 seconds

    All lanes: Western blot - Anti-CASPR3 antibody [EPR22727-102] (ab252413) at 1/1000 dilution

    All lanes: Mouse P5 cerebral cortex tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 141 kDa

    Observed band size: 150 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (ab252413), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (ab252413)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Caspr3/CNTNAP3 with ab252413 at 1/2000 (0.274 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Postive staining on mouse cerebrum (PMID: 26807827). The section was incubated with ab252413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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