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Rabbit Recombinant Monoclonal CASR antibody. Carrier free. Suitable for mIHC, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (AB280360), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (AB280360), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (AB280360), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (AB280360), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (AB280360), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCICC/IFIPFlow CytWBIHC-FrIHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Expected
Tested
Mouse
Expected
Not recommended
Not recommended
Not recommended
Tested
Tested
Tested
Rat
Expected
Not recommended
Not recommended
Not recommended
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Species

Rat

Dilution info

-

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (PubMed:7759551, PubMed:8702647, PubMed:8636323, PubMed:8878438, PubMed:17555508, PubMed:19789209, PubMed:21566075, PubMed:22114145, PubMed:23966241, PubMed:25292184, PubMed:25104082, PubMed:26386835, PubMed:25766501, PubMed:22789683). Senses fluctuations in the circulating calcium concentration and modulates the production of parathyroid hormone (PTH) in parathyroid glands (By similarity). The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:7759551). The G-protein-coupled receptor activity is activated by a co-agonist mechanism: aromatic amino acids, such as Trp or Phe, act concertedly with divalent cations, such as calcium or magnesium, to achieve full receptor activation (PubMed:27434672, PubMed:27386547).

Alternative names

Extracellular calcium-sensing receptor, CaR, CaSR, hCasR, Parathyroid cell calcium-sensing receptor 1, PCaR1, CASR, GPRC2A, PCAR1

Recommended products

Rabbit Recombinant Monoclonal CASR antibody. Carrier free. Suitable for mIHC, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR24050-59

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab280360 is the carrier-free version of Anti-CaSR antibody [EPR24050-59] ab259846.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The calcium-sensing receptor often referred to as CaSR is a critical G-protein coupled receptor involved in calcium ion homeostasis. Also known as CASR this receptor detects changes in extracellular calcium concentration and modulates signaling pathways accordingly. The CaSR protein has a molecular weight of approximately 120 kDa. It is mainly expressed in the parathyroid gland kidney and several other tissues playing a significant role in regulating serum calcium levels.

Biological function summary

The calcium-sensing receptor modulates parathyroid hormone secretion and renal calcium excretion in response to calcium fluctuations. Although not typically part of a larger protein complex it interacts with several other proteins to perform its functions efficiently. CaSR ensures the stability of calcium concentration which is essential for bone health muscle contraction and nerve function.

Pathways

Several other proteins work alongside the calcium-sensing receptor in the calcium signaling and parathyroid hormone secretion pathways. In particular CaSR influences the MAPK pathway which impacts cell growth and differentiation. Phosphate transporters and calbindins are related to CaSR within these pathways indicating its broad scope in calcium metabolism.

Associated diseases and disorders

The calcium-sensing receptor is closely associated with conditions like hypercalcemia and autosomal dominant hypocalcemia. CaSR mutations can result in dysfunctional calcium regulation impacting parathyroid hormone levels. The relationship between CaSR and parathyroid hormone-related peptide highlights its significance in maintaining calcium balance and preventing skeletal and neurological complications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360)

    This data was developed using Anti-CaSR antibody [EPR24050-59] ab259846, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human parathyroid gland tissue labeling CaSR with Anti-CaSR antibody [EPR24050-59] ab259846 at 1/5000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous and cytoplasmic staining in human parathyroid gland. The section was incubated with Anti-CaSR antibody [EPR24050-59] ab259846 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360)

    This data was developed using Anti-CaSR antibody [EPR24050-59] ab259846, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CaSR with Anti-CaSR antibody [EPR24050-59] ab259846 at 1/5000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous and cytoplasmic staining in human pancreatic islet and ducts. The section was incubated with Anti-CaSR antibody [EPR24050-59] ab259846 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360)

    This data was developed using Anti-CaSR antibody [EPR24050-59] ab259846, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CaSR with Anti-CaSR antibody [EPR24050-59] ab259846 at 1/5000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse kidney. The section was incubated with Anti-CaSR antibody [EPR24050-59] ab259846 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360)

    This data was developed using Anti-CaSR antibody [EPR24050-59] ab259846, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling CaSR with Anti-CaSR antibody [EPR24050-59] ab259846 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360)

    This data was developed using Anti-CaSR antibody [EPR24050-59] ab259846, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat parathyroid gland tissue labeling CaSR with Anti-CaSR antibody [EPR24050-59] ab259846 at 1/5000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous and cytoplasmic staining in rat parathyroid gland. The section was incubated with Anti-CaSR antibody [EPR24050-59] ab259846 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360)

    This data was developed using Anti-CaSR antibody [EPR24050-59] ab259846, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling CaSR with Anti-CaSR antibody [EPR24050-59] ab259846 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat kidney is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360)

    This data was developed using Anti-CaSR antibody [EPR24050-59] ab259846, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling CaSR with Anti-CaSR antibody [EPR24050-59] ab259846 at 1/5000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human cerebellum. The section was incubated with Anti-CaSR antibody [EPR24050-59] ab259846 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Multiplex immunohistochemistry - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360)

    Fluorescence multiplex immunohistochemical analysis of the Human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections).

    Panel A: merged staining of anti-CaSR (Anti-CaSR antibody [EPR24050-59] ab259846, magenta; Opal™690), anti-Cytochrome C (Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438, green; Opal™520) and anti-FABP4 (Anti-FABP4 antibody [EPR3579] ab92501, red; Opal™570) on human parathyroid gland. Panel B: anti-CaSR stained on parathyroid chief cells. Panel C: anti-Cytochrome C stained on parathyroid oxyphil cells. Panel D: anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

    The section was incubated in three rounds of staining: in the order of Anti-CaSR antibody [EPR24050-59] ab259846 at 1/5000 dilution (0.103 μg/ml), Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438 at 1/5000 dilution (0.195 μg/ml), and Anti-FABP4 antibody [EPR3579] ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

    This data was developed using Anti-CaSR antibody [EPR24050-59] ab259846, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360), expandable thumbnail

    Western blot - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360)

    This data was developed using Anti-CaSR antibody [EPR24050-59] ab259846, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

    Negative control: skeletal muscle.
    The molecular weight observed is consistent with literatures (PMID: 9722601, PMID: 3360311).
    Samples are non-boiled as boiling may cause protein aggregation and were run on a Bis-Tris gel.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CaSR antibody [EPR24050-59] (Anti-CaSR antibody [EPR24050-59] ab259846) at 1/1000 dilution

    Lane 1: Human kidney tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Lane 3: Mouse kidney tissue lysate at 20 µg

    Lane 4: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 5: Rat kidney tissue lysate at 20 µg

    Lane 6: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 121 kDa

    Exposure time: 15s

  • Western blot - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360), expandable thumbnail

    Western blot - Anti-CaSR antibody [EPR24050-59] - BSA and Azide free (ab280360)

    This data was developed using Anti-CaSR antibody [EPR24050-59] ab259846, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 and Anti-Integrin alpha V antibody [EPR16800] ab179475 were used as loading controls.

    The molecular weight observed is consistent with literatures (PMID: 9722601, PMID: 3360311).
    Samples are non-boiled as boiling may cause protein aggregation and were run on a Bis-Tris gel.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution and anti- Integrin alpha V antibody (Anti-Integrin alpha V antibody [EPR16800] ab179475) loading control staining at 1/5000 dilution.

    All lanes: Western blot - Anti-CaSR antibody [EPR24050-59] (Anti-CaSR antibody [EPR24050-59] ab259846) at 1/1000 dilution

    Lane 1: Mouse kidney membrane tissue lysate at 10 µg

    Lane 2: Mouse kidney membrane control tissue lysate at 10 µg

    Lane 3: Rat kidney membrane tissue lysate at 10 µg

    Lane 4: Rat skeletal muscle tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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