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AB325840

Anti-CASR antibody [HL2357] - BSA and Azide free

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Rabbit Recombinant Monoclonal CASR antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Rat, Cat, Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CASR.

View Alternative Names

GPRC2A, PCAR1, CASR, Extracellular calcium-sensing receptor, CaR, CaSR, hCasR, Parathyroid cell calcium-sensing receptor 1, PCaR1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)

ab325840 detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded human kidney. ab325840 diluted at 1 : 400. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)
  • IP

Supplier Data

Immunoprecipitation - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)

Immunoprecipitation of Calcium Sensing Receptor protein from TT whole cell extract using 5 ug of ab325840. Western blot analysis was performed using ab325840.

All lanes:

Immunoprecipitation - Anti-CASR antibody [HL2357] - BSA and Azide free (ab325840) at 5 µg

All lanes:

Calcium Sensing Receptor protein

Secondary

All lanes:

anti-rabbit IgG (HRP)

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)

ab325840 detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded mouse kidney. ab325840 diluted at 1 : 400. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)

ab325840 detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded rat kidney. ab325840 diluted at 1 : 400. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)
  • WB

Supplier Data

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ab325840 diluted at 1 : 1000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (ab325840) at 1/1000 dilution

Lane 1:

TT whole cell extracts at 30 µg

Lane 2:

293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)
  • WB

Supplier Data

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ab325840 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (ab325840) at 1/1000 dilution

Lane 1:

TT whole cell extracts at 30 µg

Lane 2:

293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)
  • WB

Supplier Data

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)

Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ab325840 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (ab325840) at 1/1000 dilution

All lanes:

Rat kidney tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)
  • WB

Supplier Data

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ab325840 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (ab325840) at 1/1000 dilution

Lane 1:

Mouse kidney tissue extracts at 50 µg

Lane 2:

Mouse liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)
  • WB

Supplier Data

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (AB325840)

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ab325840 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CASR antibody [HL2357] - BSA and Azide free (ab325840) at 1/1000 dilution

All lanes:

PG-4 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2357

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse, Human, Cat

Applications

WB, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human CASR.

P41180

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5 µg", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (PubMed : 17555508, PubMed : 19789209, PubMed : 21566075, PubMed : 22114145, PubMed : 22789683, PubMed : 23966241, PubMed : 25104082, PubMed : 25292184, PubMed : 25766501, PubMed : 26386835, PubMed : 32817431, PubMed : 33603117, PubMed : 34194040, PubMed : 34467854, PubMed : 7759551, PubMed : 8636323, PubMed : 8702647, PubMed : 8878438). Senses fluctuations in the circulating calcium concentration : activated by elevated circulating calcium, leading to decreased parathyroid hormone (PTH) secretion in parathyroid glands (By similarity). In kidneys, acts as a key regulator of renal tubular calcium resorption (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G-proteins) and modulates the activity of downstream effectors (PubMed : 38632411). CASR is coupled with different G(q)/G(11), G(i)/G(o)- or G(s)-classes of G-proteins depending on the context (PubMed : 38632411). In the parathyroid and kidney, CASR signals through G(q)/G(11) and G(i)/G(o) G-proteins : G(q)/G(11) coupling activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers, while G(i)/G(o) coupling mediates inhibition of adenylate cyclase activity (PubMed : 38632411, PubMed : 7759551). The G-protein-coupled receptor activity is activated by a co-agonist mechanism : aromatic amino acids, such as Trp or Phe, act concertedly with divalent cations, such as calcium or magnesium, to achieve full receptor activation (PubMed : 27386547, PubMed : 27434672, PubMed : 32817431, PubMed : 33603117, PubMed : 34194040). Acts as an activator of the NLRP3 inflammasome via G(i)/G(o)-mediated signaling : down-regulation of cyclic AMP (cAMP) relieving NLRP3 inhibition by cAMP (PubMed : 32843625). Acts as a regulator of proton-sensing receptor GPR68 in a seesaw manner : CASR-mediated signaling inhibits GPR68 signaling in response to extracellular calcium, while GPR68 inhibits CASR in presence of extracellular protons (By similarity).
See full target information CASR
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