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AB76933

Anti-CAST antibody

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(1 Publication)

Rabbit Polyclonal CAST antibody. Suitable for ICC, IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human POLR1G aa 400 to C-terminus.

View Alternative Names

ASE1, CAST, CD3EAP, PAF49, POLR1G, DNA-directed RNA polymerase I subunit RPA34, A34.5, Antisense to ERCC-1 protein, CD3-epsilon-associated protein, DNA-directed RNA polymerase I subunit G, RNA polymerase I-associated factor PAF49, ASE-1, CD3E-associated protein

4 Images
Immunocytochemistry - Anti-CAST antibody (AB76933)
  • ICC

Supplier Data

Immunocytochemistry - Anti-CAST antibody (AB76933)

Immunocytochemical analysis of formaldehyde-fixed asynchronous HeLa cells aataining CAST with ab76933 at 1/100 dilution and detected by Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed antibody at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAST antibody (AB76933)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAST antibody (AB76933)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PAF49 with ab76933 at 1/200 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-CAST antibody (AB76933)
  • IP

Unknown

Immunoprecipitation - Anti-CAST antibody (AB76933)

ab76933 at 1μg/ml staining CAST in HeLa whole cell lysate immunoprecipitated using ab76933 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

All lanes:

Immunoprecipitation - Anti-CAST antibody (ab76933)

Predicted band size: 55 kDa

false

Western blot - Anti-CAST antibody (AB76933)
  • WB

Unknown

Western blot - Anti-CAST antibody (AB76933)

All lanes:

Western blot - Anti-CAST antibody (ab76933) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 55 kDa

Observed band size: 130 kDa,75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, WB, ICC

applications

Immunogen

Synthetic Peptide within Human POLR1G aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15446

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p>Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab76933 was affinity purified using an epitope specific to CAST immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpastatin also known as CAST is a specific inhibitor of calpain a calcium-dependent cysteine protease. Calpastatin has an approximate mass of 86 kDa and is expressed in various tissues throughout the body including the brain and muscle tissue. Its expression ensures the tight regulation of calpain activity allowing appropriate control of cellular processes which calpain influences through proteolysis.
Biological function summary

Calpastatin acts to regulate calpain's protease activity within cells. It achieves this by directly binding with and inhibiting the activated form of calpain therefore preventing unwanted proteolysis of substrates. Calpastatin does not function as an isolated protein but often forms part of larger protein complexes. Through these interactions calpastatin modulates activities important for cellular homeostasis differentiation and apoptosis.

Pathways

Several cellular pathways integrate calpastatin function owing to its modulation of calpain activity. The calcium signaling pathway significantly involves calpastatin as its inhibition of calpain helps balance cellular responses to changes in calcium ion concentrations. Calpastatin is also closely related to the ubiquitin-proteasome pathway given that calpain can process proteins involved in this regulatory pathway influencing protein degradation and cell cycle progression.

Calpastatin has implications in muscular dystrophies and neurodegenerative diseases. In conditions like muscular dystrophy calpastatin may become dysregulated leading to excessive calpain activity that damages muscle protein structures. Calpastatin's dysregulation is also linked with Alzheimer's disease where aberrant calpain activation appears to contribute to neuronal damage. Understanding how calpastatin interacts with these pathways provides insight into potential therapeutic targets such as calpain in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Involved in UBTF-activated transcription, presumably at a step following PIC formation.. Isoform 2. Has been described as a component of preformed T-cell receptor (TCR) complex.
See full target information POLR1G
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 41:1684-97 PubMed23258707

2012

Trinucleotide repeat deletion via a unique hairpin bypass by DNA polymerase β and alternate flap cleavage by flap endonuclease 1.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Xu,Jonathan Gabison,Yuan Liu
View all publications
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Product promise

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