Anti-Catalase antibody [12C2DB9] - Peroxisome Marker

• ICC

Unknown

Immunocytochemistry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (AB110292)

Immunocytochemical staining of Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110292 (5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody, Alexa Fluor® 488 goat anti-mouse IgG (green), was used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates mainly in peroxisomes.

• Flow Cyt

Lab

Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (AB110292)

Overlay histogram showing HAP1 wildtype (green line) and HAP1-CAT knockout cells (red line) stained with ab110292. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab110292, 1μg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.

A mouse IgG1 isotype control antibody (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CAT knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

• Flow Cyt

Unknown

Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (AB110292)

Flow cytometric analysis of HL-60 cells using ab110292 at 1 µg/mL, (blue) or an isotype control (red).

• IP

Unknown

Immunoprecipitation - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (AB110292)

Immunoprecipitation of Catalase using ab110292 from :
Lane 1 : HepG2 cells
Lane 2 : Cow heart sample
Lane 3 : Rat liver sample

Predicted band size is 60 kDa.

All lanes:

Immunoprecipitation - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)

Predicted band size: 60 kDa

false