AB76110

Anti-Catalase antibody [EPR1928Y]

Name: Anti-Catalase antibody [EPR1928Y] (ab76110) | Abcam
Brand: Abcam Limited
SKU: AB76110
Price: 350 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(27 Publications)

Rabbit Recombinant Monoclonal Catalase antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 27 publications.

View Alternative Names

Catalase, CAT

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (AB76110)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (AB76110)

ab76110 at 1/100-1/250 dilution staining Catalase in human liver by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-Catalase antibody [EPR1928Y] (AB76110)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Catalase knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab76110 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76110 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76110 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)

Predicted band size: 60 kDa

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Unknown

Western blot - Anti-Catalase antibody [EPR1928Y] (AB76110)

All lanes:

Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110) at 1/1000 dilution

Lane 1:

TF cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

660 APC

APC Anti-Catalase antibody [EPR1928Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Catalase antibody [EPR1928Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Catalase antibody [EPR1928Y]
578 PE

PE Anti-Catalase antibody [EPR1928Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Catalase antibody [EPR1928Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Catalase antibody [EPR1928Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Catalase antibody [EPR1928Y]
Carrier free

Anti-Catalase antibody [EPR1928Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1928Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Catalase also known as CAT is an enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen. This enzyme has a molecular weight of approximately 240 kDa and typically forms a tetramer. Catalase mainly resides in peroxisomes functioning as a peroxisome marker. It expresses abundantly in the liver kidneys and erythrocytes where it plays significant roles in cellular protection against oxidative damage. The presence of Catalase makes it an ideal candidate for use in peroxisome staining and peroxisome image analysis in research.

Biological function summary

Catalase contributes to antioxidant defense by breaking down hydrogen peroxide preventing cellular damage. In peroxisomes it works alongside other peroxisomal enzymes to maintain cell health and metabolic regulation. Catalase does not form a complex but interacts closely with other enzymes like superoxide dismutase which dismutates superoxide radicals into less harmful substances. Increased Catalase activity levels can be measured using Catalase activity kits and activity assays allowing us to learn about peroxisome function.

Pathways

Hydrogen peroxide removal by Catalase is vital in the reactive oxygen species (ROS) metabolic process and plays a part in the cellular response to oxidative stress. Catalase interacts with the glutathione peroxidase pathway safeguarding cells from oxidative stress-related damage. Superoxide dismutase works synergistically with Catalase transforming superoxide anions into hydrogen peroxide before its decomposition by Catalase. These activities highlight the essential role Catalase plays in protecting cells from oxidative stress damage.

Catalase relates to conditions like acatalasemia and diabetes. Acatalasemia a condition caused by a deficiency of Catalase increases the risk of developing diabetes and other oxidative stress-related diseases. Mutations in the CAT gene can lead to decreased Catalase activity contributing to the onset of these conditions. Additionally Catalase works with other proteins like glutathione peroxidase in mitigating the effects of oxidative stress with deficiencies potentially exacerbating complications in diabetes management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide (PubMed : 7882369). Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells (PubMed : 7882369).

See full target information Catalase

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 12:1506388 PubMed40486827

2025

Yellow Wine Polyphenolic Compounds protect against myocardial ischemia-reperfusion injury in rats by activating Nrf2 nuclear translocation to regulate the balance of mitochondrial fission and fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Xu,Jiedong Zhou,Haifei Lou,Haodi Gu,Haixia Xu,Zuoquan Zhong,Hui Lin,Chengjian Jiang

The Journal of physiology 601:4699-4721 PubMed37815420

2023

Interleukin-1β triggers muscle-derived extracellular superoxide dismutase expression and protects muscles from doxorubicin-induced atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Mami Yamada,Mitsuharu Okutsu

Nutrients 15: PubMed37764716

2023

Ultrasound-Treated and Thermal-Pasteurized Hawthorn Vinegar: Antioxidant and Lipid Profiles in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Karakçı,Buket Bakır,Nilay Seyidoglu,Seydi Yıkmış

Cell biology and toxicology 39:3061-3075 PubMed37368165

2023

Tungsten toxicity on kidney tubular epithelial cells induces renal inflammation and M1-macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Jonatan Barrera-Chimal,Nathalie Henley,Michael Philip Grant,Schrodinger Cenatus,Pedro Geraldes,Vincent Pichette,Casimiro Gerarduzzi

Acta pharmacologica Sinica 44:2037-2047 PubMed37193756

2023

Peroxisome proliferator-activated receptor α agonist induces mouse hepatomegaly through the spatial hepatocyte enlargement and proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yang,Xiao Yang,Yi-Fei Zhang,Jia-Ning Tian,Shi-Cheng Fan,Yue Gao,Hui-Lin Li,Cheng-Hui Cai,Min Huang,Hui-Chang Bi

Journal of advanced research 46:87-100 PubMed37003700

2023

Persistent activation of Nrf2 in a p62-dependent non-canonical manner aggravates lead-induced kidney injury by promoting apoptosis and inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Cai-Yu Lian,Bing-Xin Chu,Wei-Hao Xia,Zhen-Yong Wang,Rui-Feng Fan,Lin Wang

Cancer research 82:3486-3498 PubMed35916672

2022

Selenium Modulates Cancer Cell Response to Pharmacologic Ascorbate.

Applications

Unspecified application

Species

Unspecified reactive species

Connor S R Jankowski,Joshua D Rabinowitz

International journal of molecular sciences 23: PubMed35628465

2022

Improvement of Glycaemia and Endothelial Function by a New Low-Dose Curcuminoid in an Animal Model of Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Oliveira,Tamaeh Monteiro-Alfredo,Rita Henriques,Carlos Fontes Ribeiro,Raquel Seiça,Teresa Cruz,Célia Cabral,Rosa Fernandes,Fátima Piedade,Maria Paula Robalo,Paulo Matafome,Sónia Silva

Frontiers in pharmacology 12:747325 PubMed35115920

2022

Kinsenoside Alleviates Alcoholic Liver Injury by Reducing Oxidative Stress, Inhibiting Endoplasmic Reticulum Stress, and Regulating AMPK-Dependent Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Limin Gao,Xingyu Chen,Zeyu Fu,Jie Yin,Yafen Wang,Weiguang Sun,Hong Ren,Yonghui Zhang

Cell death & disease 12:940 PubMed34645791

2021

PARK7 promotes repair in early steroid-induced osteonecrosis of the femoral head by enhancing resistance to stress-induced apoptosis in bone marrow mesenchymal stem cells via regulation of the Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Zhang,Yanglin Yan,Wuxun Peng,Lei Wang,Tao Wang,Zhihong Xie,Hong Luo,Jian Zhang,Wentao Dong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Catalase antibody [EPR1928Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (AB76110)

Western blot - Anti-Catalase antibody [EPR1928Y] (AB76110)

Western blot - Anti-Catalase antibody [EPR1928Y] (AB76110)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (27)

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