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Rabbit Recombinant Monoclonal Catalase antibody. Peroxisome marker. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 26 publications.


Images

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211), expandable thumbnail
  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211), expandable thumbnail
  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211), expandable thumbnail
  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Expected
Mouse
Tested
Tested
Tested
Rat
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

-

Species

Rat

Dilution info

1/2000

Notes

-

Species

Human

Dilution info

1/2000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/60

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

Alternative names

Catalase, CAT

Recommended products

Rabbit Recombinant Monoclonal Catalase antibody. Peroxisome marker. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 26 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20198

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Catalase also known as CAT is an enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen. This enzyme has a molecular weight of approximately 240 kDa and typically forms a tetramer. Catalase mainly resides in peroxisomes functioning as a peroxisome marker. It expresses abundantly in the liver kidneys and erythrocytes where it plays significant roles in cellular protection against oxidative damage. The presence of Catalase makes it an ideal candidate for use in peroxisome staining and peroxisome image analysis in research.

Biological function summary

Catalase contributes to antioxidant defense by breaking down hydrogen peroxide preventing cellular damage. In peroxisomes it works alongside other peroxisomal enzymes to maintain cell health and metabolic regulation. Catalase does not form a complex but interacts closely with other enzymes like superoxide dismutase which dismutates superoxide radicals into less harmful substances. Increased Catalase activity levels can be measured using Catalase activity kits and activity assays allowing us to learn about peroxisome function.

Pathways

Hydrogen peroxide removal by Catalase is vital in the reactive oxygen species (ROS) metabolic process and plays a part in the cellular response to oxidative stress. Catalase interacts with the glutathione peroxidase pathway safeguarding cells from oxidative stress-related damage. Superoxide dismutase works synergistically with Catalase transforming superoxide anions into hydrogen peroxide before its decomposition by Catalase. These activities highlight the essential role Catalase plays in protecting cells from oxidative stress damage.

Associated diseases and disorders

Catalase relates to conditions like acatalasemia and diabetes. Acatalasemia a condition caused by a deficiency of Catalase increases the risk of developing diabetes and other oxidative stress-related diseases. Mutations in the CAT gene can lead to decreased Catalase activity contributing to the onset of these conditions. Additionally Catalase works with other proteins like glutathione peroxidase in mitigating the effects of oxidative stress with deficiencies potentially exacerbating complications in diabetes management.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211), expandable thumbnail

    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    Lanes 1-2: Merged signal (red and green). Green - ab209211 observed at 60 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab209211 Anti-Catalase antibody [EPR20198] - Peroxisome Marker was shown to specifically react with Catalase in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human CAT (Catalase) knockout HeLa cell line ab265250 (knockout cell lysate Human CAT (Catalase) knockout HeLa cell lysate ab256859) was used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab209211 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: CAT knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (Mouse myoblast cell line) cells labeling Catalase with ab209211 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C2C12 cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211), expandable thumbnail

    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    Lanes 1 - 4: Merged signal (red and green). Green - ab209211 observed at 60 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.


    ab209211 was shown to specifically react with CAT in wild-type HAP1 cells. No band was observed when CAT knockout samples were examined. Wild-type and CAT knockout samples were subjected to SDS-PAGE. ab209211 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: CAT knockout HAP1 whole cell lysate at 20 µg

    Lane 3: Hek293 whole cell lysate at 20 µg

    Lane 4: HepG2 whole cell lysate at 20 µg

    Predicted band size: 60 kDa

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211), expandable thumbnail

    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-3: 5 seconds; Lane 4/5: 1 second; Lane 6: 3 seconds.

    All lanes: Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse spleen lysate at 10 µg

    Lane 4: Mouse kidney lysate at 10 µg

    Lane 5: Rat heart lysate at 10 µg

    Lane 6: Rat brain lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211), expandable thumbnail

    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2/3/6: 3 seconds; Lane 4/5: 30 seconds.

    All lanes: Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Lane 5: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

    Lane 6: C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211), expandable thumbnail

    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

    Exposure time: 1min

  • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    ImmunoFluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Catalase with ab209211 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed C2C12 (Mouse myoblast cell line) cells labeling Catalase with ab209211 at 1/60 dilution (red) compared withRabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    4-20% tris-glycin gel

    All lanes: Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    All lanes: SHSY5Y cell lysate at 40 µg

    Secondary

    All lanes: Anti-rabbit IgG, HRP-linked Antibody #7074 at 1/10000 dilution

    Exposure time: 12min

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    4-20% tris-glycin gel

    All lanes: Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    All lanes: Balb/C mice brain tissue lysate at 40 µg

    Secondary

    All lanes: Anti-rabbit IgG, HRP-linked Antibody #7074 at 1/10000 dilution

    Exposure time: 6min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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