Anti-Catalase antibody [EPR20198] - Peroxisome Marker

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed C2C12 (Mouse myoblast cell line) cells labeling Catalase with ab209211 at 1/60 dilution (red) compared withRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (Mouse myoblast cell line) cells labeling Catalase with ab209211 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C2C12 cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

ImmunoFluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Catalase with ab209211 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

• WB

AbReview76889****

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

4-20% tris-glycin gel

All lanes:

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

All lanes:

SHSY5Y cell lysate at 40 µg

Secondary

All lanes:

Anti-rabbit IgG, HRP-linked Antibody #7074 at 1/10000 dilution

false

Exposure time: 12min

This image is courtesy of an anonymous Abreview

• WB

Lab

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

Lanes 1-2 : Merged signal (red and green). Green - ab209211 observed at 60 kDa. Red - loading control ab8245 observed at 37 kDa.

ab209211 Anti-Catalase antibody [EPR20198] - Peroxisome Marker was shown to specifically react with Catalase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265250 (knockout cell lysate ab256859) was used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab209211 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CAT knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CAT (Catalase) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cat-catalase-knockout-hela-cell-line-ab265250'>ab265250</a>)

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

• WB

Supplier Data

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

Exposure time: 1min

• WB

Lab

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

Lanes 1 - 4 : Merged signal (red and green). Green - ab209211 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab209211 was shown to specifically react with CAT in wild-type HAP1 cells. No band was observed when CAT knockout samples were examined. Wild-type and CAT knockout samples were subjected to SDS-PAGE. ab209211 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

CAT knockout HAP1 whole cell lysate at 20 µg

Lane 3:

Hek293 whole cell lysate at 20 µg

Lane 4:

HepG2 whole cell lysate at 20 µg

Predicted band size: 60 kDa

false

• WB

Supplier Data

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 1 second; Lane 2/3/6 : 3 seconds; Lane 4/5 : 30 seconds.

All lanes:

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 5:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 6:

C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

• WB

AbReview76890****

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

4-20% tris-glycin gel

All lanes:

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

All lanes:

Balb/C mice brain tissue lysate at 40 µg

Secondary

All lanes:

Anti-rabbit IgG, HRP-linked Antibody #7074 at 1/10000 dilution

false

Exposure time: 6min

This image is courtesy of an anonymous Abreview

• WB

Supplier Data

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-3 : 5 seconds; Lane 4/5 : 1 second; Lane 6 : 3 seconds.

All lanes:

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Mouse kidney lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Lane 6:

Rat brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

• WB

CiteAb

Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

Catalase western blot using anti-Catalase antibody [EPR20198] - Peroxisome Marker ab209211. Publication image and figure legend from Peng, K. T., Tsai, M. H., et al., 2018, PLoS One, PubMed 30125327.

ab209211 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab209211 please see the product overview.

Expression of various ROS-related proteins, pro-inflammatory factors, and an osteoclast differentiation factor RANKL in tissue samples of all treated groups at 24 weeks after surgery.(A) Representative Western blot analysis of transferrin, catalase, SOD1, SOD2, SOD3, GPx1, GPx2, TTR, NF-κB, IL-1β and RANKL in different groups. GAPDH was used as internal control. (B) Densitometric quantification of Western blot analysis for all tested proteins normalized to GAPDH (n = 3 for each group). *, p < 0.05, for results compared to those of the sham group; #, p < 0.05, for results compared to those of the group receiving an artificial knee joint only.

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