Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)

Rabbit Recombinant Monoclonal Cathelicidin/CLP antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR28791-612] (AB318195), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	Flow Cyt (Intra)	WB	IP	IHC-P
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Tested
Rat	Expected	Expected	Not recommended	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Target data

See full target information Camp

Function

Antimicrobial protein that is an integral component of the innate immune system (By similarity). Binds to bacterial lipopolysaccharides (LPS) (By similarity). Cathelin-related antimicrobial peptide. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2.

Alternative names

Cnlp, Cramp, 24.1, Y10A, Cathelicidin antimicrobial peptide, Cathelin-like protein, CLP

Recommended products

Rabbit Recombinant Monoclonal Cathelicidin/CLP antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28791-612
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for rat WB.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cathelicidin also known as LL-37 peptide is an antimicrobial peptide with a mass of around 18 kDa. It plays an important role in the innate immune system by disrupting microbial membranes. LL-37 is expressed in various tissues including skin mucosal surfaces and certain immune cells. These peptides protect the host from infection by directly killing bacteria viruses and fungi and by modulating immune responses.

Biological function summary

Peptides like Cathelicidin are essential for host defense mechanisms contributing to cell signaling and pathogen clearance. LL-37 does not function within a complex but acts independently to influence immune cell chemotaxis. It promotes wound healing angiogenesis and tissue repair thereby ensuring rapid response to injury or infection.

Pathways

Cathelicidin interacts within inflammatory and wound healing pathways. It is involved in the NF-kB signaling pathway which regulates immune response and is linked to TLR (Toll-like receptor) pathways impacting cytokine production and further immune modulation. Proteins such as defensins share similar pathways indicating their collective influence in antimicrobial defense and tissue repair.

Associated diseases and disorders

Peptides like Cathelicidin are associated with conditions like psoriasis and atopic dermatitis. Overexpression or dysregulation of LL-37 can exacerbate inflammation in these autoimmune skin disorders. Related to these diseases proteins such as interleukins (IL-1 and IL-6) are often co-expressed or influenced by Cathelicidin levels indicating their intertwined role in skin homeostasis and immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Immunohistochemical analysis of paraffin-embedded Mouse pancreatic tumor tissue labeling Cathelicidin/CLP with ab318195 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on granulocytes in mouse pancreatic tumor. The section was incubated with ab318195 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
The band around 18 kDa is the immature form and 16 kDa is the cathelin-like domain processed from the immature full-length protein (PMID: 12454100, 34125490)
All lanes: Western blot - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195) at 1/1000 dilution
All lanes: Mouse bone marrow tissue lysate at 50 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 18 kDa, 16 kDa
Exposure time: 1s
Western blot - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Negative control: brain, skeletal mucle (PMID: 9148921).
The band around 18 kDa is the immature form and 16 kDa is the cathelin-like domain processed from the immature full-length protein (PMID: 12454100, 34125490)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 2: 3 seconds, lanes 1, 3-5: 180 seconds
All lanes: Western blot - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195) at 1/1000 dilution
Lane 1: Mouse lung tissue lysate at 50 µg with NFDM/TBST
Lane 2: Mouse spleen tissue lysate at 50 µg with NFDM/TBST
Lane 3: Mouse cerebral cortex tissue lysate at 50 µg with NFDM/TBST
Lane 4: Mouse skeletal muscle tissue lysate at 50 µg with NFDM/TBST
Lane 5: Mouse brain tissue lysate at 50 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 18 kDa, 16 kDa, 36 kDa
Flow Cytometry (Intracellular) - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neuron cells labelling Cathelicidin/CLP with ab318195 at 1/5000 dilution (0.01 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: mouse primary neuron
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Cathelicidin/CLP with ab318195 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat cerebrum. The section was incubated with ab318195 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Cathelicidin/CLP with ab318195 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cerebrum. The section was incubated with ab318195 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunoprecipitation - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Cathelicidin/CLP was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab318195 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318195 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse spleen tissue lysate

Lane 2: ab318195 IP in Mouse spleen tissue lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab318195 in Mouse spleen tissue
The band around 18 kDa is the immature form and 16 kDa is the cathelin-like domain processed from the immature full-length protein (PMID: 12454100, 34125490)
All lanes: Immunoprecipitation - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195) at 1/30 dilution
All lanes: Mouse spleen tissue lysate with NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s
Flow Cytometry (Intracellular) - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse bone marrow cells labelling Cathelicidin/CLP with ab318195 at 1/5000 dilution (0.01 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Cathelicidin/CLP with ab318195 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on granulocytes in rat lung. The section was incubated with ab318195 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cathelicidin/CLP with ab318195 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on granulocytes in mouse lung. The section was incubated with ab318195 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow cells labelling Cathelicidin/CLP with ab318195 at 1/500 (1.038 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in subsets of mouse bone marrow (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Cathelicidin/CLP with ab318195 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen. The section was incubated with ab318195 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Cathelicidin/CLP with ab318195 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat spleen. The section was incubated with ab318195 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-Cathelicidin/CLP antibody [EPR28791-612] (ab318195)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Cathelicidin/CLP with ab318195 at 1/500 (1.038 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Negative control: Confocal image showing negative staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.