AB190077

Anti-Cathepsin B antibody

Name: Anti-Cathepsin B antibody (ab190077) | Abcam
Brand: Abcam Limited
SKU: AB190077

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(5 Publications)

Rabbit Polyclonal Cathepsin B antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CTSB aa 150-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CPSB, CTSB, Cathepsin B, APP secretase, Cathepsin B1, APPS

4 Images

Flow Cytometry (Intracellular) - Anti-Cathepsin B antibody (AB190077)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cathepsin B antibody (AB190077)

Intracellular flow cytometric analysis of HepG2cells labeling Cathepsin B with ab190077 at 1/10 dilution (lowerhistogram) compared to a negative control (upperhistogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody (AB190077)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody (AB190077)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling Cathepsin B with ab190077 at 10 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody (AB190077)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labeling Cathepsin B with ab190077 at 10 μg/ml followed by peroxidase-conjugated secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-Cathepsin B antibody (AB190077)

All lanes:

Western blot - Anti-Cathepsin B antibody (ab190077) at 1/1000 dilution

All lanes:

Y79 cell lysate at 35 µg

Predicted band size: 38 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

Synthetic Peptide within Human CTSB aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P07858

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.

Biological function summary

Cathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.

Pathways

Cathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.

Cathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed : 12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed : 12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed : 3972105).

See full target information CTSB

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107929 PubMed39454957

2024

TFEB agonist clomiphene citrate activates the autophagy-lysosomal pathway and ameliorates Alzheimer's disease symptoms in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jieru Lin,Yi Yuan,Chunhuan Huang,Jiayu Zi,Lu Li,Jiamiao Liu,Xiaoting Wu,Wei Li,Qing Zhao,Yuyin Li,Zhenxing Liu,Aipo Diao

Frontiers in pharmacology 15:1449178 PubMed39359254

2024

The novel TFEB agonist desloratadine ameliorates hepatic steatosis by activating the autophagy-lysosome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jieru Lin,Chunhuan Huang,Jingye Zhao,Lu Li,Zhenwei Wu,Tingyu Zhang,Yuyin Li,Wei Li,Baoqiang Guo,Zhenxing Liu,Aipo Diao

Cells 11: PubMed35563761

2022

In Silico, In Vitro, and Clinical Investigations of Cathepsin B and Stefin A mRNA Expression and a Correlation Analysis in Kidney Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Rudzinska-Radecka,Anastasia S Frolova,Anastasia V Balakireva,Neonila V Gorokhovets,Vadim S Pokrovsky,Darina V Sokolova,Dmitry O Korolev,Natalia V Potoldykova,Andrey Z Vinarov,Alessandro Parodi,Andrey A Zamyatnin

Cancers 12: PubMed32455715

2020

Cysteine Cathepsins Inhibition Affects Their Expression and Human Renal Cancer Cell Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Rudzińska,Alessandro Parodi,Valentina D Maslova,Yuri M Efremov,Neonila V Gorokhovets,Vladimir A Makarov,Vasily A Popkov,Andrey V Golovin,Evgeni Y Zernii,Andrey A Zamyatnin

Biochemical pharmacology 162:191-201 PubMed30471247

2018

Clomiphene citrate induces nuclear translocation of the TFEB transcription factor and triggers apoptosis by enhancing lysosomal membrane permeabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Jieru Lin,Zhichen Shi,Jiren Wen,Yuyin Li,Zhenxing Liu,Aipo Diao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cathepsin B antibody

Flow Cytometry (Intracellular) - Anti-Cathepsin B antibody (AB190077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody (AB190077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody (AB190077)

Western blot - Anti-Cathepsin B antibody (AB190077)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise