Anti-Cathepsin B antibody [CA10]

• Flow Cyt

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Flow Cytometry - Anti-Cathepsin B antibody [CA10] (AB58802)

Overlay histogram showing HepG2 cells stained with ab58802 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58802, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

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Western blot - Anti-Cathepsin B antibody [CA10] (AB58802)

All lanes:

Western blot - Anti-Cathepsin B antibody [CA10] (ab58802) at 0.25 µg/mL

All lanes:

Western blot - Native human Cathepsin B protein (<a href='/en-us/products/proteins-peptides/native-human-cathepsin-b-protein-ab90387'>ab90387</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 38 kDa

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Exposure time: 10s