Rabbit Recombinant Monoclonal Cathepsin B antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (AB227811), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-b-antibody-epr21033-bsa-and-azide-free-ab227811--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76886.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (AB227811)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (AB227811), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-b-antibody-epr21033-bsa-and-azide-free-ab227811--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29587.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (AB227811)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (AB227811), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-b-antibody-epr21033-bsa-and-azide-free-ab227811--immunocytochemistry-immunofluorescence-img107296.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (AB227811)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (AB227811), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-b-antibody-epr21033-bsa-and-azide-free-ab227811--immunocytochemistry-immunofluorescence-img109201.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (AB227811)")
![Flow Cytometry (Intracellular) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (AB227811), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-b-antibody-epr21033-bsa-and-azide-free-ab227811--flow-cytometry-intracellular-img80102.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (AB227811)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Mouse | Expected | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected | Tested |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (By similarity). Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (PubMed:12782676). Has also been implicated in tumor invasion and metastasis (By similarity).
Alternative names
Cathepsin B, Cathepsin B1, Ctsb
Recommended products
Rabbit Recombinant Monoclonal Cathepsin B antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21033
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227811 is the carrier-free version of Anti-Cathepsin B antibody [EPR21033] ab214428.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.
- Biological function summary
Cathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.
- Pathways
Cathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.
- Associated diseases and disorders
Cathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-b-antibody-epr21033-bsa-and-azide-free-ab227811--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76886.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cathepsin B with Anti-Cathepsin B antibody [EPR21033] ab214428 at 1/1000 dilution, followed by secondary Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Punctate cytoplasmic staining on mouse kidney (PMID: 20668705; PMID: 26831567) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cathepsin B antibody [EPR21033] ab214428).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-b-antibody-epr21033-bsa-and-azide-free-ab227811--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29587.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Cathepsin B with Anti-Cathepsin B antibody [EPR21033] ab214428 at 1/1000 dilution, followed by secondary Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Punctate cytoplasmic staining on rat kidney (PMID: 20668705; PMID: 26831567) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cathepsin B antibody [EPR21033] ab214428).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-b-antibody-epr21033-bsa-and-azide-free-ab227811--immunocytochemistry-immunofluorescence-img107296.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811)")
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811)
Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling Cathepsin B with Anti-Cathepsin B antibody [EPR21033] ab214428 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cells.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cathepsin B antibody [EPR21033] ab214428).
![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-b-antibody-epr21033-bsa-and-azide-free-ab227811--immunocytochemistry-immunofluorescence-img109201.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811)")
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811)
Immunofluorescent analysis of 100% methanol-fixed PC-12 (rat adrenal gland pheochromocytoma cell line) cells labeling Cathepsin B with Anti-Cathepsin B antibody [EPR21033] ab214428 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on PC-12 cells.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cathepsin B antibody [EPR21033] ab214428).
![Flow Cytometry (Intracellular) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-b-antibody-epr21033-bsa-and-azide-free-ab227811--flow-cytometry-intracellular-img80102.jpg "Flow Cytometry (Intracellular) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811)")
Flow Cytometry (Intracellular) - Anti-Cathepsin B antibody [EPR21033] - BSA and Azide free (ab227811)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling Cathepsin B with Anti-Cathepsin B antibody [EPR21033] ab214428 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cathepsin B antibody [EPR21033] ab214428).
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