AB125067

Anti-Cathepsin B antibody [EPR4323]

RabMAb
Recombinant
20ul selling size
KO Validated
What is this?

Be the first to review this product! Submit a review

(29 Publications)

Rabbit Recombinant Monoclonal Cathepsin B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 29 publications.

View Alternative Names

CPSB, CTSB, Cathepsin B, APP secretase, Cathepsin B1, APPS

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (AB125067)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (AB125067)

ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (AB125067)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (AB125067)

ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (AB125067)

ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Collaborator

Western blot - Anti-Cathepsin B antibody [EPR4323] (AB125067)

ab125067 was shown to react with CTSB in wild-type HAP1 cells in Western blot with loss of signal observed in a CTSB knockout cell line. Wild-type HAP1 and CTSB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab125067 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution

Lane 1:

Wild-type HAP1 lysate at 30 µg

Lane 2:

CTSB knock-out HAP1 lysate at 30 µg

false

Unknown

Western blot - Anti-Cathepsin B antibody [EPR4323] (AB125067)

All lanes:

Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution

Lane 1:

HT1080 cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

SW480 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa

true

Carrier free

Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4323

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Our in‑house QC data shows only the pro–cathepsin B band, with no additional cleaved chains detected.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.

Biological function summary

Cathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.

Pathways

Cathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.

Cathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed : 12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed : 12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed : 3972105).

See full target information CTSB

style

left-column

Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 20:100 PubMed41035046

2025

MINPP1 promotes ferroptosis in HBV-related hepatocellular carcinoma by regulating CTSB K33-linked deubiquitination via ZRANB1.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbiao Chen,Yang Song,Liyang Wang,Feng Xiong,Shenggang Zhang

European journal of medical research 30:403 PubMed40394663

2025

Inhibiting cathepsin B alleviates acute lung injury caused by sepsis through suppression of pyroptosis in lung epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Zhang,Zhuojun Deng,Xinyu Zhang,Qian Xu,Li Liu,Dong Yang,Zimeng Guo

Molecular imaging and biology 26:1057-1067 PubMed39331316

2024

Immunohistochemical Evaluation of Cathepsin B, L, and S Expression in Breast Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Daan G J Linders,Okker D Bijlstra,Laura C Fallert,N Geeske Dekker-Ensink,Taryn L March,Martin Pool,Ethan Walker,Brian Straight,James P Basilion,Matthew Bogyo,Jacobus Burggraaf,Denise E Hilling,Alexander L Vahrmeijer,Peter J K Kuppen,A Stijn L P Crobach

npj aging 10:24 PubMed38704365

2024

EPB41L4A-AS1 is required to maintain basal autophagy to modulates Aβ clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqiang Wang,Ruomei Wang,Lixin Niu,Xiaoyan Zhou,Jinxiang Han,Kun Li

Cancer research communications 4:970-985 PubMed38517140

2024

Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Hirani,Jacqueline McDermott,Vinothini Rajeeve,Pedro R Cutillas,J Louise Jones,Daniel J Pennington,Thomas N Wight,Salvatore Santamaria,Kimberly M Alonge,Oliver M T Pearce

Journal of orthopaedic surgery and research 18:877 PubMed37980493

2023

Circ_0020014 mediates CTSB expression and participates in IL-1β-prompted chondrocyte injury via interacting with miR-24-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Chenpeng Zhang,Wenjun He

Chemical biology & drug design 102:1409-1420 PubMed37599208

2023

Hederagenin inhibits high glucose-induced fibrosis in human renal cells by suppression of NLRP3 inflammasome activation through reducing cathepsin B expression.

Applications

Unspecified application

Species

Unspecified reactive species

Guohua Yang,Wang Yang,Hairong Jiang,Qing Yi,Wei Ma

Molecular cell 83:2524-2539.e7 PubMed37390818

2023

Lysosomal LAMP proteins regulate lysosomal pH by direct inhibition of the TMEM175 channel.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyuan Zhang,Weizhong Zeng,Yan Han,Wan-Ru Lee,Jen Liou,Youxing Jiang

Cell 185:2292-2308.e20 PubMed35750034

2022

Parkinson's disease-risk protein TMEM175 is a proton-activated proton channel in lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Meiqin Hu,Ping Li,Ce Wang,Xinghua Feng,Qi Geng,Wei Chen,Matangi Marthi,Wenlong Zhang,Chenlang Gao,Whitney Reid,Joel Swanson,Wanlu Du,Richard I Hume,Haoxing Xu

Cancers 14: PubMed35626132

2022

diTFPP, a Phenoxyphenol, Sensitizes Hepatocellular Carcinoma Cells to C-Ceramide-Induced Autophagic Stress by Increasing Oxidative Stress and ER Stress Accompanied by LAMP2 Hypoglycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Chih Chiu,Yen-Chun Chen,Yung-Ding Bow,Jeff Yi-Fu Chen,Wangta Liu,Jau-Ling Huang,En-De Shu,Yen-Ni Teng,Chang-Yi Wu,Wen-Tsan Chang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cathepsin B antibody [EPR4323]

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (AB125067)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (AB125067)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (AB125067)

Western blot - Anti-Cathepsin B antibody [EPR4323] (AB125067)

Western blot - Anti-Cathepsin B antibody [EPR4323] (AB125067)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (29)

Product promise