Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (AB230401)

ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125067).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (AB230401)

This IHC data was generated using the same anti-Cathepsin B antibody clone, EPR4323, in a different buffer formulation (cat# ab125067).

ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (AB230401)

ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125067).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Collaborator

Western blot - Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (AB230401)

This data was developed using ab125067, the same antibody clone in a different buffer formulation.

ab125067 was shown to react with CTSB in wild-type HAP1 cells in Western blot with loss of signal observed in a CTSB knockout cell line. Wild-type HAP1 and CTSB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab125067 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Cathepsin B antibody [EPR4323] (<a href='/en-us/products/primary-antibodies/cathepsin-b-antibody-epr4323-ab125067'>ab125067</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 lysate at 30 µg

Lane 2:

CTSB knock-out HAP1 lysate at 30 µg

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