AB217310

Anti-Cathepsin D antibody

Name: Anti-Cathepsin D antibody (ab217310) | Abcam
Brand: Abcam Limited
SKU: AB217310
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Cathepsin D antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CTSD aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CPSD, CTSD, Cathepsin D

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody (AB217310)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody (AB217310)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Skin with ab217310 at 1/200

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody (AB217310)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody (AB217310)

Immunofluorescence analysis of formalin-fixed U138 cells, labeling Cathepsin D using ab217310 at a 1/50 dilution; followed by goat anti-rabbit lgG (H+L) Alexa Fluor 488-conjugated secondary antibody.

Supplier Data

Western blot - Anti-Cathepsin D antibody (AB217310)

All lanes:

Western blot - Anti-Cathepsin D antibody (ab217310) at 1/200 dilution

Lane 1:

rat kidney lysates

Lane 2:

Human colon carcinoma lysates

Secondary

All lanes:

goat anti-rabbit antibody (H+L) HRP-conjugated at 1/3000 dilution

Predicted band size: 44 kDa

Observed band size: 43 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CTSD aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P07339

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin D also known as CTSD is a protein with a mass of approximately 45 kDa. It functions as an aspartyl protease and is expressed in lysosomes across various tissues including the liver and kidneys. This enzyme acts by cleaving peptide bonds within proteins which is essential for protein degradation and turnover. Cathepsin D exists as precursor forms that become activated in the acidic environment of the lysosome. It plays a critical role in normal cellular processes by maintaining protein homeostasis.

Biological function summary

The enzymatic activity of Cathepsin D is important for cellular maintenance and apoptosis. This protease does not act within larger protein complexes but contributes to the degradation of extracellular and intracellular proteins. It mediates processes like antigen processing where it deconstructs proteins into peptides that are presented on major histocompatibility complex (MHC) molecules. ELISA tests can quantify its expression levels sometimes termed as CTSD activity in various biological samples offering insights into its role within cellular environments.

Pathways

Cathepsin D involvement includes the lysosomal degradation pathway and the apoptotic signaling pathway. In the lysosomal degradation pathway Cathepsin D breaks down proteins and peptides a process important for cellular recycling and energy release. It interacts with other lysosomal enzymes such as Cathepsin B in this pathway ensuring comprehensive breakdown of cellular waste. The apoptotic signaling pathway involves the regulation of programmed cell death where Cathepsin D can influence the activation of downstream proteins like Bcl-2 and Bax which control cell survival.

The overexpression of Cathepsin D links to breast cancer and Alzheimer's disease. In breast cancer increased Cathepsin D expression correlates with tumor progression and metastasis influencing tumor behavior through interactions with other proteins involved in cell proliferation. Alzheimer's disease features the involvement of Cathepsin D in the breakdown of amyloid precursor protein which relates to amyloid beta plaque accumulation. The abnormal activity of Cathepsin D in these disorders makes it a potential target for therapeutic antibodies such as CTSD antibodies which aim to regulate its function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed : 27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

See full target information CTSD

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell stress & chaperones 26:433-441 PubMed33471265

2021

Effect of Nrf2 signaling pathway on the improvement of intestinal epithelial barrier dysfunction by hyperbaric oxygen treatment after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xuehua Liu,Fang Liang,Wei Song,Xiaoli Diao,Wanqiu Zhu,Jing Yang

Journal of proteome research 18:3099-3118 PubMed31265301

2019

Proteomic Analysis of Rat Cerebral Cortex in the Subacute to Long-Term Phases of Focal Cerebral Ischemia-Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Meiling Wen,Ya Jin,Hao Zhang,Xiaoou Sun,Yihe Kuai,Wen Tan

Biotechnology and applied biochemistry 66:555-563 PubMed30994947

2019

Cilostazol protects against myocardial ischemia and reperfusion injury by activating transcription factor EB (TFEB).

Applications

Unspecified application

Species

Unspecified reactive species

Jiangjin Li,Xiaoli Xiang,Zuo Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cathepsin D antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody (AB217310)

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody (AB217310)

Western blot - Anti-Cathepsin D antibody (AB217310)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise