AB6313

Anti-Cathepsin D antibody [CTD-19]

Name: Anti-Cathepsin D antibody [CTD-19] Mouse monoclonal (ab6313) | Abcam
Brand: Abcam Limited
SKU: AB6313
Price: 625 USD
Availability: InStock
Rating: 5 (16 reviews)

(16 Reviews)

(84 Publications)

Mouse Monoclonal Cathepsin D antibody. Suitable for WB and reacts with Human samples. Cited in 84 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CTSD.

View Alternative Names

CPSD, CTSD, Cathepsin D

2 Images

Unknown

Western blot - Anti-Cathepsin D antibody [CTD-19] (AB6313)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution

All lanes:

MCF7 Human breast carcinoma cells

Predicted band size: 44 kDa

false

AbReview14627****

Western blot - Anti-Cathepsin D antibody [CTD-19] (AB6313)

Blocked with 5% milk for 1 hour at 22°C.

Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution

All lanes:

MDA MB 231 whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution

Predicted band size: 44 kDa

Observed band size: 28 kDa,48 kDa,50 kDa

true

Exposure time: 2min

This image is courtesy of an anonymous Abreview

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CTD-19

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Native Full Length Protein corresponding to Human CTSD.

P07339

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Purified from TCS

Storage buffer

Preservative: 0.097% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin D also known as CTSD is a protein with a mass of approximately 45 kDa. It functions as an aspartyl protease and is expressed in lysosomes across various tissues including the liver and kidneys. This enzyme acts by cleaving peptide bonds within proteins which is essential for protein degradation and turnover. Cathepsin D exists as precursor forms that become activated in the acidic environment of the lysosome. It plays a critical role in normal cellular processes by maintaining protein homeostasis.

Biological function summary

The enzymatic activity of Cathepsin D is important for cellular maintenance and apoptosis. This protease does not act within larger protein complexes but contributes to the degradation of extracellular and intracellular proteins. It mediates processes like antigen processing where it deconstructs proteins into peptides that are presented on major histocompatibility complex (MHC) molecules. ELISA tests can quantify its expression levels sometimes termed as CTSD activity in various biological samples offering insights into its role within cellular environments.

Pathways

Cathepsin D involvement includes the lysosomal degradation pathway and the apoptotic signaling pathway. In the lysosomal degradation pathway Cathepsin D breaks down proteins and peptides a process important for cellular recycling and energy release. It interacts with other lysosomal enzymes such as Cathepsin B in this pathway ensuring comprehensive breakdown of cellular waste. The apoptotic signaling pathway involves the regulation of programmed cell death where Cathepsin D can influence the activation of downstream proteins like Bcl-2 and Bax which control cell survival.

The overexpression of Cathepsin D links to breast cancer and Alzheimer's disease. In breast cancer increased Cathepsin D expression correlates with tumor progression and metastasis influencing tumor behavior through interactions with other proteins involved in cell proliferation. Alzheimer's disease features the involvement of Cathepsin D in the breakdown of amyloid precursor protein which relates to amyloid beta plaque accumulation. The abnormal activity of Cathepsin D in these disorders makes it a potential target for therapeutic antibodies such as CTSD antibodies which aim to regulate its function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed : 27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

See full target information CTSD

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Publications (84)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed40279672

2025

Lysosomal TPC2 channels disrupt Ca2+ entry and dopaminergic function in models of LRRK2-Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Gregori,Gustavo J S Pereira,Robert Allen,Nicholas West,Kai-Yin Chau,Xinjiang Cai,Matthew P Bostock,Stephen R Bolsover,Marco Keller,Chiao-Yin Lee,Si Hang Lei,Kirsten Harvey,Franz Bracher,Christian Grimm,Gaiti Hasan,Matthew E Gegg,Anthony H V Schapira,Sean T Sweeney,Sandip Patel

Journal of cellular and molecular medicine 28:e18402 PubMed39008328

2024

Exacerbation of atherosclerosis by STX17 knockdown: Unravelling the role of autophagy and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Cui,Bo Wang,Dongjian Han,Mengdie Cheng,Peiyu Yuan,Pengchong Du,Yachen Hou,Chang Su,Junnan Tang,Jinying Zhang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23720 PubMed38837708

2024

Impaired cathepsin D in retinal pigment epithelium cells mediates Stargardt disease pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Eunice Sze Yin Ng,Jane Hu,Zhichun Jiang,Roxana A Radu

Autophagy reports 3:2326241 PubMed40395533

2024

Simultaneous activation and blockade of autophagy to fight hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hala Shalhoub,Patrick Gonzalez,Alexandre Dos Santos,Julie Guillermet-Guibert,Nicolas Moniaux,Nicolas Dupont,Jamila Faivre

Biomaterials 302:122316 PubMed37738741

2023

Cell-specific alterations in autophagy-lysosomal activity near the chronically implanted microelectrodes.

Applications

Unspecified application

Species

Unspecified reactive species

Keying Chen,Camila Garcia Padilla,Kirill Kiselyov,Takashi D Y Kozai

The Journal of clinical investigation 133: PubMed37384409

2023

Reduction of nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Leon Tejwani,Youngseob Jung,Hiroshi Kokubu,Sowmithra Sowmithra,Luhan Ni,Changwoo Lee,Benjamin Sanders,Paul J Lee,Yangfei Xiang,Kimberly Luttik,Armand Soriano,Jennifer Yoon,Junhyun Park,Hannah H Ro,Hyoungseok Ju,Clara Liao,Sofia Massaro Tieze,Frank Rigo,Paymaan Jafar-Nejad,Janghoo Lim

Molecular therapy. Nucleic acids 32:937-948 PubMed37346979

2023

DNAJB6 isoform specific knockdown: Therapeutic potential for limb girdle muscular dystrophy D1.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew R Findlay,May M Paing,Jil A Daw,Meade Haller,Rocio Bengoechea,Sara K Pittman,Shan Li,Feng Wang,Timothy M Miller,Heather L True,Tsui-Fen Chou,Conrad C Weihl

Science translational medicine 15:eade6285 PubMed37099634

2023

Sleep deprivation exacerbates microglial reactivity and Aβ deposition in a -dependent manner in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Samira Parhizkar,Grace Gent,Yun Chen,Nicholas Rensing,Maud Gratuze,Gregory Strout,Sanja Sviben,Eric Tycksen,Qiang Zhang,Petra Erdmann Gilmore,Robert Sprung,Jim Malone,Wei Chen,Javier Remolina Serrano,Xin Bao,Choonghee Lee,Chanung Wang,Eric Landsness,James Fitzpatrick,Michael Wong,Reid Townsend,Marco Colonna,Robert E Schmidt,David M Holtzman

The Journal of biological chemistry 299:104663 PubMed37003503

2023

Microtubule-associated protein MAP1LC3C regulates lysosomal exocytosis and induces zinc reprogramming in renal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Verma,Parul Aggarwal,Megan E Bischoff,James Reigle,Dina Secic,Collin Wetzel,Katherine VandenHeuvel,Jacek Biesiada,Birgit Ehmer,Julio A Landero Figueroa,David R Plas,Mario Medvedovic,Jarek Meller,Maria F Czyzyk-Krzeska

Journal of cell science 136: PubMed36861884

2023

StARD9 is a novel lysosomal kinesin required for membrane tubulation, cholesterol transport and Purkinje cell survival.

Applications

Unspecified application

Species

Unspecified reactive species

Felicity R Sterling,Jon D'Amico,Alexandria M Brumfield,Kara L Huegel,Patricia S Vaughan,Kathryn Morris,Shelby Schwarz,Michelle V Joyce,Bill Boggess,Matthew M Champion,Kevin Maciuba,Philip Allen,Eric Marasco,Grant Koch,Peter Gonzalez,Shannon Hodges,Shannon Leahy,Erica Gerstbauer,Edward H Hinchcliffe,Kevin T Vaughan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cathepsin D antibody [CTD-19]

Western blot - Anti-Cathepsin D antibody [CTD-19] (AB6313)

Western blot - Anti-Cathepsin D antibody [CTD-19] (AB6313)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (84)

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Product promise