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Chicken Recombinant Monoclonal Cathepsin D antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (AB302646), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (AB302646), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (AB302646), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (AB302646), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (AB302646), expandable thumbnail

Key facts

Isotype
IgY
Host species
Chicken
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Tested
Expected
Tested
Tested
Not recommended
Rat
Tested
Expected
Expected
Expected
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed:27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Alternative names

CPSD, CTSD, Cathepsin D

Recommended products

Chicken Recombinant Monoclonal Cathepsin D antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgY
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3057Y-CkIgY
Purification technique
Affinity purification Thiophilic Resin
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This chicken monoclonal chimeric antibody has been engineered from RabMab parent antibody (Anti-Cathepsin D antibody [EPR3057Y] ab75852). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using across absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cathepsin D also known as CTSD is a protein with a mass of approximately 45 kDa. It functions as an aspartyl protease and is expressed in lysosomes across various tissues including the liver and kidneys. This enzyme acts by cleaving peptide bonds within proteins which is essential for protein degradation and turnover. Cathepsin D exists as precursor forms that become activated in the acidic environment of the lysosome. It plays a critical role in normal cellular processes by maintaining protein homeostasis.

Biological function summary

The enzymatic activity of Cathepsin D is important for cellular maintenance and apoptosis. This protease does not act within larger protein complexes but contributes to the degradation of extracellular and intracellular proteins. It mediates processes like antigen processing where it deconstructs proteins into peptides that are presented on major histocompatibility complex (MHC) molecules. ELISA tests can quantify its expression levels sometimes termed as CTSD activity in various biological samples offering insights into its role within cellular environments.

Pathways

Cathepsin D involvement includes the lysosomal degradation pathway and the apoptotic signaling pathway. In the lysosomal degradation pathway Cathepsin D breaks down proteins and peptides a process important for cellular recycling and energy release. It interacts with other lysosomal enzymes such as Cathepsin B in this pathway ensuring comprehensive breakdown of cellular waste. The apoptotic signaling pathway involves the regulation of programmed cell death where Cathepsin D can influence the activation of downstream proteins like Bcl-2 and Bax which control cell survival.

Associated diseases and disorders

The overexpression of Cathepsin D links to breast cancer and Alzheimer's disease. In breast cancer increased Cathepsin D expression correlates with tumor progression and metastasis influencing tumor behavior through interactions with other proteins involved in cell proliferation. Alzheimer's disease features the involvement of Cathepsin D in the breakdown of amyloid precursor protein which relates to amyloid beta plaque accumulation. The abnormal activity of Cathepsin D in these disorders makes it a potential target for therapeutic antibodies such as CTSD antibodies which aim to regulate its function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CTSD (cathepsin D) with ab302646 at 1/10000 (0.08 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic with granular staining on human liver. The section was incubated with ab302646 for 30 mins at room temperature, followed by Anti-Chicken IgY antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CTSD (cathepsin D) with ab302646 at 1/10000 (0.08 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic with granular staining on human breast carcinoma. The section was incubated with ab302646 for 30 mins at room temperature, followed by Anti-Chicken IgY antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling CTSD (cathepsin D) with ab302646 at 1/100 (8.1 ug/ml ) dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line is observed. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling CTSD (cathepsin D) with ab302646 at 1/100 (8.1 ug/ml ) dilution, followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line is observed. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Flow Cytometry (Intracellular) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling CTSD (cathepsin D) with ab302646 at 1/1000 dilution (0.1ug) (Red) compared with a Chicken IgY (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Chicken IgY H&L (Alexa Fluor® 488, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling CTSD (cathepsin D) with ab302646 at 1/1000 dilution (0.1ug) (Red) compared with a Chicken IgY (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Chicken IgY H&L (Alexa Fluor® 488, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646), expandable thumbnail

    Western blot - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds.

    All lanes: Western blot - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

    Lane 5: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 6: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 7: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Lane 8: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (Goat Anti-Chicken IgY H&L (HRP) ab6877) at 1/20000 dilution

    Observed band size: 14 kDa, 28 kDa, 46 kDa

    Exposure time: 5.5s

  • Western blot - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646), expandable thumbnail

    Western blot - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Western blot - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (ab302646) at 1/1000 dilution

    Lane 1: Human heart tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Rat stomach tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (Goat Anti-Chicken IgY H&L (HRP) ab6877) at 1/20000 dilution

    Observed band size: 14 kDa, 28 kDa, 46 kDa

    Exposure time: 10s

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Product protocols

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