Skip to main content

Rabbit Monoclonal Cathepsin E antibody. Suitable for I-ELISA, IHC-P, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

Be the first to review this product! Submit a review

Images

Indirect ELISA - Anti-Cathepsin E antibody [EPR29973-715] (AB322657), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (AB322657), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (AB322657), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (AB322657), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (AB322657), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAIHC-PWB
Human
Expected
Tested
Tested
Mouse
Expected
Tested
Tested
Recombinant fragment - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

62.5 ng/mL

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.

Alternative names

Rabbit Monoclonal Cathepsin E antibody. Suitable for I-ELISA, IHC-P, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Indirect ELISA - Anti-Cathepsin E antibody [EPR29973-715] (ab322657), expandable thumbnail

    Indirect ELISA - Anti-Cathepsin E antibody [EPR29973-715] (ab322657)

    Indirect ELISA analysis of ab322657 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.

    Antigen: Human Cathepsin E, Human Cathepsin D, Human BACE-2.

    Antigen concentration: 1000 ng/ml

    This antibody does not cross-react with Human Cathepsin D or BACE-2.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Cathepsin E with ab322657 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in mouse liver.

    The section was incubated with ab322657 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Cathepsin E with ab322657 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in mouse pancreas.

    The section was incubated with ab322657 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Cathepsin E with ab322657 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human liver.

    The section was incubated with ab322657 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657)

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Cathepsin E with ab322657 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in mouse stomach.

    The section was incubated with ab322657 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657)

    Immunohistochemical analysis of paraffin-embedded A Human pancreas cancer B Adjacent pancreas tissue labeling Cathepsin E with ab322657 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in A: Human pancreas cancer and no staining in B: Adjacent pancreas (PMID: 33815409).

    The section was incubated with ab322657 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody [EPR29973-715] (ab322657)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Cathepsin E with ab322657 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human stomach.

    The section was incubated with ab322657 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Western blot - Anti-Cathepsin E antibody [EPR29973-715] (ab322657), expandable thumbnail

    Western blot - Anti-Cathepsin E antibody [EPR29973-715] (ab322657)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: liver

    Dimerization of two identical 42 kDa subunits of Cathepsin E results in the formation of the approximately 84 kDa mature enzyme(PMID: 7789521).

    In Western Blot, we detect bands at ~39 KDa and ~42 KDa. Human CTSE gene has several splicing variants. Based on the immunogen sequence, this antibody is expected to detect isoforms P14091-1 and P14091-2 (PMID: 12531480).

    Mouse CTSE gene has 1 potential isoform.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Cathepsin E antibody [EPR29973-715] (ab322657) at 1/1000 dilution

    Lane 1: Mouse stomach tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Human stomach tissue lysate at 20 µg

    Lane 4: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 39 kDa, 42 kDa, 78-84 kDa, 36 kDa

    Exposure time: 1s

  • Western blot - Anti-Cathepsin E antibody [EPR29973-715] (ab322657), expandable thumbnail

    Western blot - Anti-Cathepsin E antibody [EPR29973-715] (ab322657)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: DLD-1, LoVo(PMID: 23451082)

    Dimerization of two identical 42 kDa subunits of Cathepsin E results in the formation of the approximately 84 kDa mature enzyme(PMID: 7789521).

    The identity of the lower MW band in lanes 2-3 are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Cathepsin E antibody [EPR29973-715] (ab322657) at 1/1000 dilution

    Lane 1: KATO III (human stomach spherical) whole cell lysate at 20 µg

    Lane 2: DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 42 kDa, 84 kDa, 36 kDa

    Exposure time: 10s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com