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AB200650

Anti-Cathepsin F antibody

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(3 Publications)

Rabbit Polyclonal Cathepsin F antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CTSF aa 250-350.

View Alternative Names

Cathepsin F, CATSF, CTSF

1 Images
Western blot - Anti-Cathepsin F antibody (AB200650)
  • WB

Supplier Data

Western blot - Anti-Cathepsin F antibody (AB200650)

All lanes:

Western blot - Anti-Cathepsin F antibody (ab200650)

Lane 1:

HEK293T whole cell lysate

Lane 2:

Raw264.7 whole cell lysate

Lane 3:

H9C2 whole cell lysate

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CTSF aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q9UBX1

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purity of ab200650 is > 95% (by SDS-PAGE).
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin F also known as CTSF is a cysteine protease enzyme with a molecular mass of approximately 44 kDa. It belongs to the papain-like protease family and exhibits proteolytic activity. This protein localizes to the lysosomes where it participates in protein degradation. Cathepsin F shows expression in various tissues including liver kidney and spleen suggesting a broad role in protein catabolism.
Biological function summary

Cathepsin F functions in protein breakdown processes within the lysosomal compartment. It actively participates in the formation and maturation of the autophagosomes. This protease is not known to be a component of a multi-protein complex but it works in concert with other lysosomal enzymes to ensure efficient catabolic activity. Its activity helps in the turnover of intracellular proteins maintaining cellular homeostasis.

Pathways

Cathepsin F contributes significantly to the lysosomal degradation pathway. It assists in proteolytic cascades that help in breaking down proteins into amino acids. This degradation pathway is important for the recycling of cellular components and energy production. Moreover Cathepsin F shares functional relationships with other proteases like Cathepsin B and Cathepsin L as they often have overlapping substrate specificities and contribute to a similar biological pathway.

Cathepsin F has links to neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease Cathepsin F's altered activity affects protein clearance associated with amyloid beta aggregation. This protease also relates to Cancer Progression through its role in matrix remodeling. In neurodegenerative conditions and cancer Cathepsin F's interaction with proteins like Cathepsin S has implications for disease progression. Understanding these connections highlights Cathepsin F's importance in diagnosing and potentially targeting therapeutic interventions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
See full target information CTSF

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Genes to cells : devoted to molecular & cellular mechanisms 29:328-336 PubMed38366711

2024

Cathepsin L prevents the accumulation of alpha-synuclein fibrils in the cell.

Applications

Unspecified application

Species

Unspecified reactive species

Ayumi Matsuki,Yoshihisa Watanabe,Sho Hashimoto,Atsushi Hoshino,Satoaki Matoba

Aging cell 21:e13707 PubMed36087066

2022

The lysosomal proteome of senescent cells contributes to the senescence secretome.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Rovira,Rebecca Sereda,David Pladevall-Morera,Valentina Ramponi,Ines Marin,Mate Maus,Julio Madrigal-Matute,Antonio Díaz,Fernando García,Javier Muñoz,Ana María Cuervo,Manuel Serrano

Stem cell reports 13:380-393 PubMed31378672

2019

A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Birket,Sophie Raibaud,Miriam Lettieri,Antony D Adamson,Valerie Letang,Pauline Cervello,Nicolas Redon,Gwenaelle Ret,Sandra Viale,Bing Wang,Bruno Biton,Jean-Claude Guillemot,Vincent Mikol,John P Leonard,Neil A Hanley,Cecile Orsini,Jean-Michel Itier
View all publications

Product promise

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