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AB36161

Anti-Cathepsin F antibody

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(1 Publication)

Goat Polyclonal Cathepsin F antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CTSF aa 450 to C-terminus.

View Alternative Names

Cathepsin F, CATSF, CTSF

1 Images
Western blot - Anti-Cathepsin F antibody (AB36161)
  • WB

Unknown

Western blot - Anti-Cathepsin F antibody (AB36161)

Primary incubation was 1 hour. Detected by chemiluminescence. Experiments gave bands at approx 30kDa and 35kDa in Human Prostate and Ovary lysates after 0.3µg/ml antibody staining. These bands correspond to earlier findings in literature (Wang et al, J Biol Chem. 1998 Nov 27;273(48) : 32000-8; PMID : 9822672). This protein has a calculated MW of 53.4kDa according to NP_003784.2.

All lanes:

Western blot - Anti-Cathepsin F antibody (ab36161) at 0.3 µg/mL

All lanes:

Human Ovary lysate (35µg protein in RIPA buffer).

Predicted band size: 53 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CTSF aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UBX1

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin F also known as CTSF is a cysteine protease enzyme with a molecular mass of approximately 44 kDa. It belongs to the papain-like protease family and exhibits proteolytic activity. This protein localizes to the lysosomes where it participates in protein degradation. Cathepsin F shows expression in various tissues including liver kidney and spleen suggesting a broad role in protein catabolism.
Biological function summary

Cathepsin F functions in protein breakdown processes within the lysosomal compartment. It actively participates in the formation and maturation of the autophagosomes. This protease is not known to be a component of a multi-protein complex but it works in concert with other lysosomal enzymes to ensure efficient catabolic activity. Its activity helps in the turnover of intracellular proteins maintaining cellular homeostasis.

Pathways

Cathepsin F contributes significantly to the lysosomal degradation pathway. It assists in proteolytic cascades that help in breaking down proteins into amino acids. This degradation pathway is important for the recycling of cellular components and energy production. Moreover Cathepsin F shares functional relationships with other proteases like Cathepsin B and Cathepsin L as they often have overlapping substrate specificities and contribute to a similar biological pathway.

Cathepsin F has links to neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease Cathepsin F's altered activity affects protein clearance associated with amyloid beta aggregation. This protease also relates to Cancer Progression through its role in matrix remodeling. In neurodegenerative conditions and cancer Cathepsin F's interaction with proteins like Cathepsin S has implications for disease progression. Understanding these connections highlights Cathepsin F’s importance in diagnosing and potentially targeting therapeutic interventions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
See full target information CTSF

Publications (1)

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Biochimica et biophysica acta 1833:2254-66 PubMed23684953

2013

N-terminally truncated forms of human cathepsin F accumulate in aggresome-like inclusions.

Applications

Unspecified application

Species

Human

Barbara Jerič,Iztok Dolenc,Marko Mihelič,Martina Klarić,Tina Zavašnik-Bergant,Gregor Gunčar,Boris Turk,Vito Turk,Veronika Stoka
View all publications

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