AB239506

Anti-Cathepsin K antibody [C1]

Name: Anti-Cathepsin K antibody [C1] (ab239506) | Abcam
Brand: Abcam Limited
SKU: AB239506
Price: 525 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal Cathepsin K antibody. Suitable for WB and reacts with Rat, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CTSK.

View Alternative Names

CTSO, CTSO2, CTSK, Cathepsin K, Cathepsin O, Cathepsin O2, Cathepsin X

5 Images

Supplier Data

Western blot - Anti-Cathepsin K antibody [C1] (AB239506)

All lanes:

Western blot - Anti-Cathepsin K antibody [C1] (ab239506) at 3 µg/mL

All lanes:

Rat skeletal muscle lysate

Predicted band size: 37 kDa

false

Supplier Data

Western blot - Anti-Cathepsin K antibody [C1] (AB239506)

All lanes:

Western blot - Anti-Cathepsin K antibody [C1] (ab239506) at 2 µg/mL

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Human lung lysate

Secondary

All lanes:

HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 37 kDa

false

Supplier Data

Western blot - Anti-Cathepsin K antibody [C1] (AB239506)

All lanes:

Western blot - Anti-Cathepsin K antibody [C1] (ab239506) at 3 µg/mL

All lanes:

Pig heart lysate

Predicted band size: 37 kDa

false

Supplier Data

Western blot - Anti-Cathepsin K antibody [C1] (AB239506)

All lanes:

Western blot - Anti-Cathepsin K antibody [C1] (ab239506) at 2 µg/mL

All lanes:

Recombinant human Cathepsin K protein

Secondary

All lanes:

HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 37 kDa

false

Supplier Data

Western blot - Anti-Cathepsin K antibody [C1] (AB239506)

All lanes:

Western blot - Anti-Cathepsin K antibody [C1] (ab239506) at 3 µg/mL

All lanes:

Pig uterus lysate

Predicted band size: 37 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Rat, Human, Pig

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CTSK.

P43235

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin K also known as CTSK is a protease enzyme that belongs to the papain family of cysteine proteases. With a molecular weight of approximately 37 kDa Cathepsin K is mainly expressed in osteoclasts which are cells involved in bone resorption. This enzyme functions by cleaving collagen a vital component of bone extracellular matrix which facilitates bone remodeling and turnover. Known for its collagenolytic activity Cathepsin K is important in processes where breakdown of collagen fibers is required.

Biological function summary

Cathepsin K plays a significant role in bone metabolism. It operates within the osteoclasts where it degrades type I collagen and other matrix proteins essential for normal bone maintenance and repair. Cathepsin K does not function as part of a complex but acts independently in its enzymatic role. It is highly specific to substrates found in the bone matrix differentiating it from other cathepsins like cathepsin L or B that may have broader expression and role.

Pathways

Cathepsin K is an important player in the bone remodeling pathway. This pathway involves a series of coordinated actions between osteoclasts and osteoblasts to maintain bone health. Cathepsin K's activity is tightly regulated within this pathway to ensure proper bone density and structure. It is associated with other proteins such as osteopontin and matrix metalloproteinases which work together to modulate bone turnover. Within the RANK/RANKL pathway Cathepsin K acts as a downstream effector following osteoclast activation by RANKL.

Cathepsin K is most notably linked to osteoporosis and pycnodysostosis. Osteoporosis arises due to excessive bone resorption where elevated Cathepsin K activity leads to weakened bone structure increasing fracture risk. Pycnodysostosis is a rare genetic disorder caused by mutations in the gene coding for Cathepsin K resulting in impaired bone resorption and abnormally dense but brittle bones. In these conditions proteins such as RANKL and osteoprotegerin also play critical roles by influencing osteoclast differentiation and activity thereby modulating Cathepsin K's effects.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (PubMed : 11082042).

See full target information CTSK

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 12:e70123 PubMed39578098

2024

Glenn circulation causes early and progressive shunting in a surgical model of pulmonary arteriovenous malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Tina C Wan,Henry Rousseau,Carol Mattern,Madeline Tabor,Matthew R Hodges,Ramani Ramchandran,Andrew D Spearman

Drug design, development and therapy 17:691-706 PubMed36915642

2023

Integrated Network Pharmacology and Experimental Validation Approach to Investigate the Mechanisms of in the Treatment of Rheumatoid Arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Wendong Xie,Hua Yang,Chun Guo,Rui Xie,Guoliang Yu,Yifu Li

Annals of translational medicine 9:1474 PubMed34734026

2021

Induction of osteoclast formation by LOX mutant (LOXG473A) through regulation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Chenglin Luo,Wenjin Xiao

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Cathepsin K antibody [C1]

Western blot - Anti-Cathepsin K antibody [C1] (AB239506)

Western blot - Anti-Cathepsin K antibody [C1] (AB239506)

Western blot - Anti-Cathepsin K antibody [C1] (AB239506)

Western blot - Anti-Cathepsin K antibody [C1] (AB239506)

Western blot - Anti-Cathepsin K antibody [C1] (AB239506)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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