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AB300569

Anti-Cathepsin K antibody [EPR24829-101]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal Cathepsin K antibody. Suitable for IHC-P, IHC-Fr and reacts with Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

CTSO, CTSO2, CTSK, Cathepsin K, Cathepsin O, Cathepsin O2, Cathepsin X

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Cathepsin K with ab300569 at 1/1000 (0.529 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on mouse kidney (PMID : 8647859). The section was incubated with ab300569 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

Immunohistochemical analysis of paraffin-embedded Mouse bone tissue labeling Cathepsin K with ab300569 at 1/1000 (0.529 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on osteoclasts in mouse bone (PMID : 29322325). The section was incubated with ab300569 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

Immunohistochemical analysis of paraffin-embedded Rat bone tissue labeling Cathepsin K with ab300569 at 1/1000 (0.529 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on osteoclasts in rat bone (PMID : 29322325). The section was incubated with ab300569 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : no staining on rat liver (PMID : 25356585). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat P0 bone (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on osteoclasts of rat P0 bone is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

  • Carrier free

    Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Cathepsin K antibody [EPR24829-101]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Cathepsin K antibody [EPR24829-101]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24829-101

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We do not recommend this antibody for mouse IHC-Fr.

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Secondary Antibodies

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin K also known as CTSK is a protease enzyme that belongs to the papain family of cysteine proteases. With a molecular weight of approximately 37 kDa Cathepsin K is mainly expressed in osteoclasts which are cells involved in bone resorption. This enzyme functions by cleaving collagen a vital component of bone extracellular matrix which facilitates bone remodeling and turnover. Known for its collagenolytic activity Cathepsin K is important in processes where breakdown of collagen fibers is required.
Biological function summary

Cathepsin K plays a significant role in bone metabolism. It operates within the osteoclasts where it degrades type I collagen and other matrix proteins essential for normal bone maintenance and repair. Cathepsin K does not function as part of a complex but acts independently in its enzymatic role. It is highly specific to substrates found in the bone matrix differentiating it from other cathepsins like cathepsin L or B that may have broader expression and role.

Pathways

Cathepsin K is an important player in the bone remodeling pathway. This pathway involves a series of coordinated actions between osteoclasts and osteoblasts to maintain bone health. Cathepsin K's activity is tightly regulated within this pathway to ensure proper bone density and structure. It is associated with other proteins such as osteopontin and matrix metalloproteinases which work together to modulate bone turnover. Within the RANK/RANKL pathway Cathepsin K acts as a downstream effector following osteoclast activation by RANKL.

Cathepsin K is most notably linked to osteoporosis and pycnodysostosis. Osteoporosis arises due to excessive bone resorption where elevated Cathepsin K activity leads to weakened bone structure increasing fracture risk. Pycnodysostosis is a rare genetic disorder caused by mutations in the gene coding for Cathepsin K resulting in impaired bone resorption and abnormally dense but brittle bones. In these conditions proteins such as RANKL and osteoprotegerin also play critical roles by influencing osteoclast differentiation and activity thereby modulating Cathepsin K's effects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (PubMed : 11082042).
See full target information CTSK
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of dental sciences 20:2399-2406 PubMed41040543

2025

Lunar life drives jawbone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Kotaro Sato,Naoka Kouyama,Shinya Akatsuka,Yashiro Motooka,Qinhong Wang,Hideharu Hibi,Shinya Toyokuni

JCI insight 9: PubMed39171525

2024

Sclerostin antibody corrects periodontal disease in type 2 diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hakan Turkkahraman,Shannan Flanagan,Tianli Zhu,Nisreen Akel,Silvia Marino,Dayane Ortega-Gonzalez,Xue Yuan,Teresita Bellido

Dental traumatology : official publication of International Association for Dental Traumatology 40:178-186 PubMed37731288

2023

Effect of intra-alveolar delivery of Frondoside A on inflammatory response of delayed tooth replantation.

Applications

Unspecified application

Species

Unspecified reactive species

Lar Herr,Ju Ri Ye,Sang Wook Kang,Sang Tae Ro,Yong Kwon Chae,Ko Eun Lee,Mi Sun Kim,Myeong Kwan Jih,Chunui Lee,Sung Chul Choi,Ok Hyung Nam

Cell death & disease 14:298 PubMed37127752

2023

A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Na Zhao,Ling-Fei Zhang,Zhen Sun,Li-Hua Qiao,Tao Yang,Yi-Zhe Ren,Xian-Zhou Zhang,Lei Wu,Wen-Li Qian,Qiao-Mei Guo,Wan-Xing Xu,Xue-Qing Wang,Fei Wu,Lin Wang,Yutong Gu,Mo-Fang Liu,Jia-Tao Lou

International journal of molecular sciences 24: PubMed36835229

2023

Dual Role of Interleukin-20 in Different Stages of Osteoclast Differentiation and Its Osteoimmune Regulation during Alveolar Bone Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Meng,Benyi Yang,Yan Qu,Yuanbo Liu,Dongle Wu,Chaoran Fu,Yifan He,Xi Chen,Chufeng Liu,Xiaoxing Kou,Yang Cao
View all publications
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Product promise

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