Rabbit Monoclonal Cathepsin K antibody. Suitable for IHC-P, IHC-Fr and reacts with Mouse, Rat samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-k-antibody-epr24829-101-ab300569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400719.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-k-antibody-epr24829-101-ab300569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400720.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-k-antibody-epr24829-101-ab300569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400721.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)")
![Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-k-antibody-epr24829-101-ab300569--immunohistochemistry-frozen-sections-img400787.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)")
![Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-k-antibody-epr24829-101-ab300569--immunohistochemistry-frozen-sections-img400788.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)")
Publications
Filter by
- Cell death & disease 14:2982023A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Na Zhao,Ling-Fei Zhang,Zhen Sun,Li-Hua Qiao,Tao Yang,Yi-Zhe Ren,Xian-Zhou Zhang,Lei Wu,Wen-Li Qian,Qiao-Mei Guo,Wan-Xing Xu,Xue-Qing Wang,Fei Wu,Lin Wang,Yutong Gu,Mo-Fang Liu,Jia-Tao LouPubMed 37127752
- International journal of molecular sciences 24:2023Dual Role of Interleukin-20 in Different Stages of Osteoclast Differentiation and Its Osteoimmune Regulation during Alveolar Bone Remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBowen Meng,Benyi Yang,Yan Qu,Yuanbo Liu,Dongle Wu,Chaoran Fu,Yifan He,Xi Chen,Chufeng Liu,Xiaoxing Kou,Yang CaoPubMed 36835229
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Tested | Tested | Not recommended | Not recommended | Not recommended |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Alternative names
CATK_HUMAN, CTS02, CTSK, CTSO, CTSO1, CTSO2, Cathepsin O, Cathepsin O1, Cathepsin O2, Cathepsin X, MGC23107, PKND, PYCD
Recommended products
Rabbit Monoclonal Cathepsin K antibody. Suitable for IHC-P, IHC-Fr and reacts with Mouse, Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24829-101
- Purification technique
- Affinity purification Protein A
- Specificity
We do not recommend this antibody for mouse IHC-Fr.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cathepsin K also known as CTSK is a protease enzyme that belongs to the papain family of cysteine proteases. With a molecular weight of approximately 37 kDa Cathepsin K is mainly expressed in osteoclasts which are cells involved in bone resorption. This enzyme functions by cleaving collagen a vital component of bone extracellular matrix which facilitates bone remodeling and turnover. Known for its collagenolytic activity Cathepsin K is important in processes where breakdown of collagen fibers is required.
- Biological function summary
Cathepsin K plays a significant role in bone metabolism. It operates within the osteoclasts where it degrades type I collagen and other matrix proteins essential for normal bone maintenance and repair. Cathepsin K does not function as part of a complex but acts independently in its enzymatic role. It is highly specific to substrates found in the bone matrix differentiating it from other cathepsins like cathepsin L or B that may have broader expression and role.
- Pathways
Cathepsin K is an important player in the bone remodeling pathway. This pathway involves a series of coordinated actions between osteoclasts and osteoblasts to maintain bone health. Cathepsin K's activity is tightly regulated within this pathway to ensure proper bone density and structure. It is associated with other proteins such as osteopontin and matrix metalloproteinases which work together to modulate bone turnover. Within the RANK/RANKL pathway Cathepsin K acts as a downstream effector following osteoclast activation by RANKL.
- Associated diseases and disorders
Cathepsin K is most notably linked to osteoporosis and pycnodysostosis. Osteoporosis arises due to excessive bone resorption where elevated Cathepsin K activity leads to weakened bone structure increasing fracture risk. Pycnodysostosis is a rare genetic disorder caused by mutations in the gene coding for Cathepsin K resulting in impaired bone resorption and abnormally dense but brittle bones. In these conditions proteins such as RANKL and osteoprotegerin also play critical roles by influencing osteoclast differentiation and activity thereby modulating Cathepsin K's effects.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-k-antibody-epr24829-101-ab300569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400719.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569)
Immunohistochemical analysis of paraffin-embedded Mouse bone tissue labeling Cathepsin K with ab300569 at 1/1000 (0.529 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on osteoclasts in mouse bone (PMID: 29322325). The section was incubated with ab300569 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-k-antibody-epr24829-101-ab300569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400720.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569)
Immunohistochemical analysis of paraffin-embedded Rat bone tissue labeling Cathepsin K with ab300569 at 1/1000 (0.529 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on osteoclasts in rat bone (PMID: 29322325). The section was incubated with ab300569 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-k-antibody-epr24829-101-ab300569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400721.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Cathepsin K with ab300569 at 1/1000 (0.529 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: no staining on mouse kidney (PMID: 8647859). The section was incubated with ab300569 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-k-antibody-epr24829-101-ab300569--immunohistochemistry-frozen-sections-img400787.jpg "Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569)")
Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat P0 bone (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on osteoclasts of rat P0 bone is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
.![Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-k-antibody-epr24829-101-ab300569--immunohistochemistry-frozen-sections-img400788.jpg "Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569)")
Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (ab300569)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on rat liver (PMID: 25356585). is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
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