AB58991

Anti-Cathepsin L/MEP antibody

Name: Anti-Cathepsin L/MEP antibody (ab58991) | Abcam
Brand: Abcam Limited
SKU: AB58991
Rating: 5 (4 reviews)

(4 Reviews)

(37 Publications)

Rabbit Polyclonal CPL1 antibody. Suitable for WB and reacts with Caenorhabditis elegans samples. Cited in 37 publications.

View Alternative Names

T03E6.7, cpl-1, Cathepsin L-like

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Caenorhabditis elegans

Applications

applications

Specificity

Reacts specifically with Cathepsin L/MEP protease 36 kDa wild type protein of C.elegans

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Caenorhabditis elegans": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin L also known as CTSL is a protease enzyme that has a mass of approximately 29-30 kDa. It originates from the peptidase C1 family and undergoes activation in acidic environments. This protein carries out proteolytic processes by breaking down proteins through cleaving peptide bonds. Cathepsin L has various forms including MEP (a common alternate name) and MEP L and it expresses itself in organs such as the liver kidney and spleen as well as in tumors and immune cells. It has a significant functional role in lysosomes where it degrades proteins.

Biological function summary

Cathepsin L links to cellular homeostasis and extracellular matrix remodeling. It often acts in protein turnover and antigen processing within endolysosomal compartments making it essential for major histocompatibility complex class II presentation. Cathepsin L forms complexes in certain conditions playing roles in interacting and modifying other proteins. It controls processes essential for cell survival differentiation and apoptosis.

Pathways

Cathepsin L plays significant roles in pathways like apoptosis and autophagy. It coordinates with other proteases and proteins such as cathepsin B and cathepsin S to regulate cell death and survival. In apoptosis cathepsin L mediates the breakdown of cellular components working alongside caspases. Its interaction with autophagy involves degradation of long-lived proteins highlighting its role in recycling amino acids during stress conditions.

Cathepsin L connects strongly to cancer and fibrotic diseases. It contributes to tumor progression and metastasis due to its ability to degrade the extracellular matrix and enable cancer cell invasion. In fibrosis cathepsin L modulates the turnover of fibrous tissue linking to the development of lung fibrotic diseases. Proteins like collagenases work in concert with cathepsin L during these pathological processes to remodel tissues underlining its implication in disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cysteine protease which plays an essential role in the degradation of proteins in lysosomes (PubMed : 15456850, PubMed : 24829385). During early embryogenesis, maternally required for the proteolytic processing of yolk proteins in platelets, a lysosome-like structure where a slow and controlled degradation of yolk proteins occurs (PubMed : 15456850, PubMed : 24829385). In the gonad, required for the clearance of apoptotic germ cells in the engulfing cell phagolysosomes (PubMed : 24829385). In embryos, required for the degradation of endocytic and autophagic cargos (PubMed : 24829385). In embryos, may play a role in the degradation of lipid-containing droplets (PubMed : 26773047). Required for larval development (PubMed : 11707440, PubMed : 15456850).

See full target information cpl-1

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:183 PubMed38360932

2024

The Drosophila ZNRF1/2 homologue, detour, interacts with HOPS complex and regulates autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Shannon Nicolson,Jantina A Manning,Yoon Lim,Xin Jiang,Erica Kolze,Sonia Dayan,Ruchi Umargamwala,Tianqi Xu,Jarrod J Sandow,Andrew I Webb,Sharad Kumar,Donna Denton

Scientific reports 14:3200 PubMed38331993

2024

The Ykt6-Snap29-Syx13 SNARE complex promotes crinophagy via secretory granule fusion with Lamp1 carrier vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Győző Szenci,Gábor Glatz,Szabolcs Takáts,Gábor Juhász

Life science alliance 6: PubMed37316298

2023

PI3P-dependent regulation of cell size and autophagy by phosphatidylinositol 5-phosphate 4-kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Avishek Ghosh,Aishwarya Venugopal,Dhananjay Shinde,Sanjeev Sharma,Meera Krishnan,Swarna Mathre,Harini Krishnan,Sankhanil Saha,Padinjat Raghu

Autophagy 19:2682-2701 PubMed37289040

2023

PtdIns4P exchange at endoplasmic reticulum-autolysosome contacts is essential for autophagy and neuronal homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Liu,Wenxia Shao,Wei Liu,Weina Shang,Jun-Ping Liu,Liquan Wang,Chao Tong

International journal of molecular sciences 24: PubMed37175806

2023

The Small-Molecule Enhancers of Autophagy AUTEN-67 and -99 Delay Ageing in Striated Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marcell Komlós,Janka Szinyákovics,Gergő Falcsik,Tímea Sigmond,Bálint Jezsó,Tibor Vellai,Tibor Kovács

Cell reports 41:111583 PubMed36323251

2022

ER-mitochondrial contact protein Miga regulates autophagy through Atg14 and Uvrag.

Applications

Unspecified application

Species

Unspecified reactive species

Lingna Xu,Yunyi Qiu,Xufeng Wang,Weina Shang,Jian Bai,Kexin Shi,Hao Liu,Jun-Ping Liu,Liquan Wang,Chao Tong

Cell reports 38:110286 PubMed35081354

2022

Selective autophagy controls innate immune response through a TAK1/TAB2/SH3PX1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Panagiotis Tsapras,Stavroula Petridi,Selina Chan,Marta Geborys,Anne-Claire Jacomin,Antonia P Sagona,Pascal Meier,Ioannis P Nezis

Journal of virology 96:e0021522 PubMed35608346

2022

Identification of Cellular Genes Involved in Baculovirus GP64 Trafficking to the Plasma Membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey J Hodgson,Nicolas Buchon,Gary W Blissard

Autophagy 18:2443-2458 PubMed35266854

2022

Lamp1 mediates lipid transport, but is dispensable for autophagy in .

Applications

Unspecified application

Species

Unspecified reactive species

Norin Chaudhry,Margaux Sica,Satya Surabhi,David Sanchez Hernandez,Ana Mesquita,Adem Selimovic,Ayesha Riaz,Laury Lescat,Hua Bai,Gustavo C MacIntosh,Andreas Jenny

International journal of molecular sciences 22: PubMed34638976

2021

Drosophila Rab39 Attenuates Lysosomal Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Zsolt Lakatos,Péter Benkő,Gábor Juhász,Péter Lőrincz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Cathepsin L/MEP antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (37)

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