Rabbit Recombinant Monoclonal Cathepsin L/MEP antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Images
![Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (AB323652), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-mep-antibody-epr29413-573-bsa-and-azide-free-capture-ab323652--sandwich-elisa-img444478.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (AB323652)")
![Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (AB323652), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-mep-antibody-epr29413-573-bsa-and-azide-free-capture-ab323652--sandwich-elisa-img444479.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (AB323652)")
![Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (AB323652), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-mep-antibody-epr29413-573-bsa-and-azide-free-capture-ab323652--sandwich-elisa-img444480.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (AB323652)")
![Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (AB323652), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-mep-antibody-epr29413-573-bsa-and-azide-free-capture-ab323652--sandwich-elisa-img444477.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (AB323652)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Mouse | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (PubMed:12782676). In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter (PubMed:12869695). In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells (PubMed:12021314). Also mediates invariant chain processing in cortical thymic epithelial cells (PubMed:9545226). Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation (PubMed:12417635). Secreted form generates endostatin from COL18A1 (PubMed:10716919). Critical for cardiac morphology and function (PubMed:11972068). Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation (PubMed:12163394). Required for maximal stimulation of steroidogenesis by TIMP1 (By similarity).
Alternative names
Ctsl1, Ctsl, Procathepsin L, Cathepsin L1, Major excreted protein, p39 cysteine proteinase, MEP
Rabbit Recombinant Monoclonal Cathepsin L/MEP antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29413-573
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab323652 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Cathepsin L/MEP. The recommended pair for sandwich ELISA is:
Capture: ab323652, Mouse Cathepsin L/MEP Capture Antibody (unconjugated)
Detector: Anti-Cathepsin L/MEP antibody [EPR29413-594] - BSA and Azide free (Detector) ab323653, Mouse Cathepsin L/MEP Detector Antibody (unconjugated)
The reference range value is 39.06-5000 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-mep-antibody-epr29413-573-bsa-and-azide-free-capture-ab323652--sandwich-elisa-img444478.jpg "Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652)")
Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652)
Interpolated concentration of native Cathepsin L/MEP was measured at different sample concentrations. Undiluted sample is as follows: mouse plasma 100%. Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-mep-antibody-epr29413-573-bsa-and-azide-free-capture-ab323652--sandwich-elisa-img444479.jpg "Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652)")
Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652)
Interpolated concentration of native Cathepsin L/MEP was measured at different sample concentrations. Undiluted sample is as follows: NIH/3T3 supernatant (3 days) 50%. The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-mep-antibody-epr29413-573-bsa-and-azide-free-capture-ab323652--sandwich-elisa-img444480.jpg "Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652)")
Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652)
Interpolated concentration of native Cathepsin L/MEP was measured at different sample concentrations. Undiluted sample is as follows: NIH/3T3 cell extract (125 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-mep-antibody-epr29413-573-bsa-and-azide-free-capture-ab323652--sandwich-elisa-img444477.jpg "Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652)")
Sandwich ELISA - Anti-Cathepsin L/MEP antibody [EPR29413-573] - BSA and Azide free (Capture) (ab323652)
Sandwich ELISA with the capture antibody ab323652 dilution at 2 ug/mL and detector antibody Anti-Cathepsin L/MEP antibody [EPR29413-594] - BSA and Azide free (Detector) ab323653 dilution at 0.5 ug/mL.
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