Mouse Monoclonal Cathepsin L/MEP antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 34 publications.
Images
![Western blot - Anti-Cathepsin L + V antibody [33/2] (AB6314), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-antibody-33-2-ab6314--western-blot-img138477.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L + V antibody [33/2] (AB6314)")
![Western blot - Anti-Cathepsin L + V antibody [33/2] (AB6314), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-antibody-33-2-ab6314--western-blot-img153008.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L + V antibody [33/2] (AB6314)")
![Western blot - Anti-Cathepsin L + V antibody [33/2] (AB6314), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-antibody-33-2-ab6314--western-blot-img113611.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L + V antibody [33/2] (AB6314)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L + V antibody [33/2] (AB6314), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-antibody-33-2-ab6314--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117856.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L + V antibody [33/2] (AB6314)")
![Western blot - Anti-Cathepsin L + V antibody [33/2] (AB6314), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-antibody-33-2-ab6314--western-blot-img133774.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L + V antibody [33/2] (AB6314)")
Publications
Filter by
- Cell death and differentiation 29:2137-21502022S-Nitrosylation of cathepsin B affects autophagic flux and accumulation of protein aggregates in neurodegenerative disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKi-Ryeong Kim,Eun-Jung Cho,Jae-Won Eom,Sang-Seok Oh,Tomohiro Nakamura,Chang-Ki Oh,Stuart A Lipton,Yang-Hee KimPubMed 35462559
- Acta pharmacologica Sinica 43:850-8612021Long-term running exercise alleviates cognitive dysfunction in APP/PSEN1 transgenic mice via enhancing brain lysosomal function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Wang,Yu-Ting Zhu,Yi Zhu,Yan-Ling Sun,Jun Huang,Zhe Li,Yan Wang,Jun-Chao Wu,Zheng-Hong Qin,Fang LinPubMed 34272505
- BMC cancer 21:5412021Cancer-associated mutations reveal a novel role for EpCAM as an inhibitor of cathepsin-L and tumor cell invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNarendra V Sankpal,Taylor C Brown,Timothy P Fleming,John M Herndon,Anusha A Amaravati,Allison N Loynd,William E GillandersPubMed 33980181
- The Journal of physiology 599:963-9792020The mechanisms of skeletal muscle atrophy in response to transient knockdown of the vitamin D receptor in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph J Bass,Abid A Kazi,Colleen S Deane,Asif Nakhuda,Stephen P Ashcroft,Matthew S Brook,Daniel J Wilkinson,Bethan E Phillips,Andrew Philp,Janelle Tarum,Fawzi Kadi,Ditte Andersen,Amadeo Muñoz Garcia,Ken Smith,Iain J Gallagher,Nathaniel J Szewczyk,Mark E Cleasby,Philip J AthertonPubMed 33258480
- Acta pharmacologica Sinica :2020Enhanced lysosomal function is critical for paclitaxel resistance in cancer cells: reversed by artesunate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhe Li et. al.PubMed 32704040
- Frontiers in pharmacology 11:6182020BRD4 Inhibition Protects Against Acute Pancreatitis Through Restoring Impaired Autophagic Flux.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuangjun Shen et. al.PubMed 32457617
- Journal of neurosurgery :1-132019Disruption of P2X4 purinoceptor and suppression of the inflammation associated with cerebral aneurysm formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiyuki Fukuda et. al.PubMed 31860812
- Frontiers in oncology 9:12392019Expression and Clinical Implications of Cysteine Cathepsins in Gallbladder Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiddharth Mehra et. al.PubMed 31824841
- The Journal of biological chemistry 294:9973-99842019C-terminal α-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan P McGlinchey et. al.PubMed 31092553
- CNS neuroscience & therapeutics 25:796-8072019Exercise activates lysosomal function in the brain through AMPK-SIRT1-TFEB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Huang et. al.PubMed 30864262
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Expected |
| Rat | Not recommended | Predicted | Predicted |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Abcam recommends using 3% milk as the blocking agent. |
Species Human | Dilution info 1/1000 | Notes Abcam recommends using 3% milk as the blocking agent. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (By similarity). In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter (By similarity). In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells (By similarity). Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation (By similarity). Secreted form generates endostatin from COL18A1 (PubMed:10716919). Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation (By similarity). Required for maximal stimulation of steroidogenesis by TIMP1 (By similarity). (Microbial infection) In cells lacking TMPRSS2 expression, facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via a slow acid-activated route with the proteolysis of coronavirus spike (S) glycoproteins in lysosome for entry into host cell (PubMed:16339146, PubMed:18562523, PubMed:32142651, PubMed:32221306, PubMed:37990007). Proteolysis within lysosomes is sufficient to activate membrane fusion by coronaviruses SARS-CoV and EMC (HCoV-EMC) S as well as Zaire ebolavirus glycoproteins (PubMed:16081529, PubMed:18562523, PubMed:26953343). Isoform 2. Functions in the regulation of cell cycle progression through proteolytic processing of the CUX1 transcription factor (PubMed:15099520). Translation initiation at downstream start sites allows the synthesis of isoforms that are devoid of a signal peptide and localize to the nucleus where they cleave the CUX1 transcription factor and modify its DNA binding properties (PubMed:15099520).
Additional Targets
CTSV
Alternative names
CTSL1, CTSL, Procathepsin L, Cathepsin L1, Major excreted protein, MEP
Recommended products
Mouse Monoclonal Cathepsin L/MEP antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 34 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 33/2
- Purification technique
- Affinity purification Protein G
- Specificity
Reacts specifically with the native and denatured forms of human cathepsin L (25kD) and procathepsin L (42 kD). Does not react with human cathepsin types B1, D1, H and S1, procathepsins B and D1 and recombinant procathepsin H, rat cathepsin B1, and mouse procathepsins B1 and D1.
- Epitope
- Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cathepsin L also referred to as CTSL and Cathepsin V known as Cathepsin L2 or CTSV are lysosomal cysteine proteases that work in the proteolytic processing of proteins. Cathepsin L has an approximate mass of 37 kDa while Cathepsin V is slightly larger. Both enzymes localize mainly to lysosomes but they also occur in secretory vesicles and extracellular spaces under specific conditions. Expression of Cathepsin L spans various tissues including the liver spleen and kidney. Cathepsin V expression the highest in the thymus and testis and less so in other tissues.
- Biological function summary
Cathepsin L and Cathepsin V play essential roles in protein degradation and turnover. They do not form stable complexes but can transiently interact with specific inhibitors to modulate their activity. These proteases maintain cellular homeostasis and assist in antigen processing facilitating immune responses. In particular Cathepsin V shows a more specialized role in extracellular matrix remodeling due to its broad substrate specificity and tissue distribution.
- Pathways
Cathepsin L and Cathepsin V are active in the lysosomal degradation pathway. They collaborate with proteases such as Cathepsin B and Cathepsin S. Cathepsin L is also important for MHC class II-mediated antigen presentation affecting immune signaling. Due to its ability to process hormone precursors Cathepsin L impacts the insulin signaling pathway sharing functional relations with proteases like furin and prohormone convertases.
- Associated diseases and disorders
Cathepsin L associates with cancer progression and metastasis due to its role in matrix degradation and cell invasion. Overexpression or aberrant activity of Cathepsin L links to invasive cancer phenotypes. Cathepsin V implicates autoimmune diseases related to antigen processing defects particularly affecting thymic selection processes. Both proteases also have indirect connections to Alzheimer’s disease pathology due to their interactions with proteins such as ß-amyloid precursors.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-antibody-33-2-ab6314--western-blot-img138477.jpg "Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)")
Image courtesy of Ratnakar Singh by customer review.Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
All lanes: Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/750 dilution
All lanes: whole cell lysate prepared from U87MG human glioblastoma at 50 µg
SecondaryAll lanes: Goat anti-mouse HRP at 1/2000 dilution
Predicted band size: 38 kDa
![Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-antibody-33-2-ab6314--western-blot-img153008.jpg "Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)")
Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
Blocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Observed MW:30
Exposure time:3 minutes
Lane 1: Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/20 dilution
Lane 1: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/20 dilution
Lane 2: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/100 dilution
Lane 2: Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
Lane 3: Anti-His tag at 1/10000 dilution
All lanes: Human Cathepsin L recombinant protein fraction
SecondaryLanes 1 and 3: Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
Lane 2: Rabbit anti-Mouse IgG at 1/2000 dilution
Predicted band size: 38 kDa
![Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-antibody-33-2-ab6314--western-blot-img113611.jpg "Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)")
Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
Blocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Observed MW:30
Exposure time:3 minutes
Lane 1: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/20 dilution
Lane 1: Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/20 dilution
Lanes 2 and 4: Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
Lanes 2 and 4: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/100 dilution
Lane 3: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/500 dilution
Lane 3: Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/500 dilution
Lane 5: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/1000 dilution
Lane 5: Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/1000 dilution
Lane 6: Anti-His tag at 1/10000 dilution
All lanes: Human Cathepsin V recombinant protein fraction
SecondaryLanes 1 - 3: Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
Lanes 4 - 5: Rabbit Anti-Mouse secondary ab at 1/2000 dilution
Lane 6: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L + V antibody [33/2] (ab6314), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-antibody-33-2-ab6314--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117856.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L + V antibody [33/2] (ab6314)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L + V antibody [33/2] (ab6314)
IHC image of Cathepsin L staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6314, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
![Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-antibody-33-2-ab6314--western-blot-img133774.jpg "Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)")
Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
All lanes: Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/1000 dilution
Lane 1: Lung (Human) Tissue Lysate at 20 µg
Lane 2: Lung (Mouse) Tissue Lysate at 20 µg
Lane 3: Human kidney tissue lysate - total protein (ab30203) at 20 µg
Lane 4: Kidney (Mouse) Tissue Lysate at 20 µg
Lane 5: A549 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 25 kDa, 55 kDa
Exposure time: 20min
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