Rabbit Recombinant Monoclonal Cathepsin L/MEP antibody. Carrier free. Suitable for IHC-P, ICC/IF, ELISA, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Synthetic peptide - Human, Recombinant fragment - Human samples.
Images
![ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (AB248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--elisa-img137242.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (AB248589)")
![Flow Cytometry (Intracellular) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (AB248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--flow-cytometry-intracellular-img38682.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (AB248589)")
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (AB248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img153008.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (AB248589)")
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (AB248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img113611.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (AB248589)")
![ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (AB248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--elisa-img137157.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (AB248589)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | ELISA | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested | Expected |
| Rat | Expected | Expected | Expected | Tested | Expected |
| Recombinant fragment - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Synthetic peptide - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Recombinant fragment - Human | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Version
Target data
Function
Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (By similarity). In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter (By similarity). In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells (By similarity). Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation (By similarity). Secreted form generates endostatin from COL18A1 (PubMed:10716919). Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation (By similarity). Required for maximal stimulation of steroidogenesis by TIMP1 (By similarity). (Microbial infection) In cells lacking TMPRSS2 expression, facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via a slow acid-activated route with the proteolysis of coronavirus spike (S) glycoproteins in lysosome for entry into host cell (PubMed:16339146, PubMed:18562523, PubMed:32142651, PubMed:32221306, PubMed:37990007). Proteolysis within lysosomes is sufficient to activate membrane fusion by coronaviruses SARS-CoV and EMC (HCoV-EMC) S as well as Zaire ebolavirus glycoproteins (PubMed:16081529, PubMed:18562523, PubMed:26953343). Isoform 2. Functions in the regulation of cell cycle progression through proteolytic processing of the CUX1 transcription factor (PubMed:15099520). Translation initiation at downstream start sites allows the synthesis of isoforms that are devoid of a signal peptide and localize to the nucleus where they cleave the CUX1 transcription factor and modify its DNA binding properties (PubMed:15099520).
Alternative names
CTSL1, CTSL, Procathepsin L, Cathepsin L1, Major excreted protein, MEP
Recommended products
Rabbit Recombinant Monoclonal Cathepsin L/MEP antibody. Carrier free. Suitable for IHC-P, ICC/IF, ELISA, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Synthetic peptide - Human, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8011
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 4.4 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248589 is the carrier-free version of Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cathepsins L V K and H are essential cysteine proteases that serve important functions in protein catabolism. These proteases participate in proteolytic processes by digesting proteins into shorter peptides and amino acids. Cathepsin L also known as CTSL typically has a molecular mass of about 25-35 kDa and is expressed in various tissues such as the liver spleen and placenta. Cathepsin K plays a similar role in bone resorption and is highly expressed in osteoclasts. Cathepsin V and Cathepsin H meanwhile have distinct tissue distributions with cathepsin V often found in the thymus and testis whereas cathepsin H's expression spans across different cell types contributing a vast array of biological functions.
- Biological function summary
Cathepsins regulate cellular homeostasis and modulate various physiological processes. These proteases perform their functions within the lysosomal compartment although they can also participate in extracellular matrix remodeling and other non-lysosomal roles. Cathepsin K for example is part of the cathepsin protein family and aids in degrading collagen therefore playing a pivotal role in bone turnover and resorption. Cathepsins can act independently or in some cases are part of larger enzyme complexes enhancing their proteolytic activity to handle different substrates driven by the physiological demand.
- Pathways
Cathepsins key in the lysosomal degradation pathway and influence other signaling cascades like the apoptotic pathway. The regulation of these pathways often involves interactions with other proteins such as pro-apoptotic members of the Bcl-2 family facilitating cell death under certain conditions. Cathepsin L contributes to the antigen processing pathway linking it to the immune response. Cathepsin K on the other hand acts closely with structural proteins like collagen in the bone metabolism pathway highlighting its significance in skeletal development and maintenance.
- Associated diseases and disorders
Altered cathepsin activity closely associates with conditions like osteoporosis and cancer. Cathepsin K upregulation can lead to excessive bone degradation seen in osteoporosis while aberrant cathepsin L expression relates to cancer progression and metastasis due to its role in degrading extracellular matrix components facilitating tumor invasion. In osteoporosis interactions with matrix metalloproteinases further enhance bone matrix breakdown exacerbating disease symptoms. In cancer the relationship between cathepsin L and the urokinase-type plasminogen activator system highlights its role in promoting malignancy and suggests potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
14 product images
![ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--elisa-img137242.jpg "ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.Antigen: CATL_HUMAN, CATV_HUMAN, CATS_HUMAN, CATK_HUMAN, CATH_HUMAN Antigen concentration: 1000ng/ml Primary antibody concentration range: 0~1000ng/ml Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) Secondary antibody concentration: 1/2500
![Flow Cytometry (Intracellular) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--flow-cytometry-intracellular-img38682.jpg "Flow Cytometry (Intracellular) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Flow Cytometry (Intracellular) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.
Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641 staining Cathepsin L in the human cell line HepG2 (human hepatocellular carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img153008.jpg "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Observed MW: 30.
Exposure time: 3 minutes.
Lane 1: Western blot - Anti-Cathepsin L + V antibody [33/2] (Anti-Cathepsin L + V antibody [33/2] ab6314) at 1/20 dilution
Lane 1: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/20 dilution
Lane 2: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/100 dilution
Lane 2: Western blot - Anti-Cathepsin L + V antibody [33/2] (Anti-Cathepsin L + V antibody [33/2] ab6314) at 1/100 dilution
Lane 3: Anti-His tag at 1/10000 dilution
All lanes: Human Cathepsin L recombinant protein fraction
SecondaryLanes 1 and 3: Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
Lane 2: Rabbit anti-Mouse IgG at 1/2000 dilution
Predicted band size: 38 kDa
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img113611.jpg "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Observed MW: 30.
Exposure time: 3 minutes.
Lane 1: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/20 dilution
Lane 1: Western blot - Anti-Cathepsin L + V antibody [33/2] (Anti-Cathepsin L + V antibody [33/2] ab6314) at 1/20 dilution
Lanes 2 and 4: Western blot - Anti-Cathepsin L + V antibody [33/2] (Anti-Cathepsin L + V antibody [33/2] ab6314) at 1/100 dilution
Lanes 2 and 4: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/100 dilution
Lane 3: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/500 dilution
Lane 3: Western blot - Anti-Cathepsin L + V antibody [33/2] (Anti-Cathepsin L + V antibody [33/2] ab6314) at 1/500 dilution
Lane 5: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/1000 dilution
Lane 5: Western blot - Anti-Cathepsin L + V antibody [33/2] (Anti-Cathepsin L + V antibody [33/2] ab6314) at 1/1000 dilution
Lane 6: Anti-His tag at 1/10000 dilution
All lanes: Human Cathepsin V recombinant protein fraction
SecondaryLanes 1 - 3: Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
Lanes 4 - 5: Rabbit Anti-Mouse secondary ab at 1/2000 dilution
Lane 6: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
![ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--elisa-img137157.jpg "ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.
Direct ELISA antigen dose-response curve using purified Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641. Antigen concentration of 1000ng/mL.Alkaline Phosphatase conjugated AffiniPure goat anti-rabbit IgG(H+L)(1/2500) was used as the secondary antibody.
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img83583.jpg "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/20000 dilution
All lanes: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img122816.jpg "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/10000 dilution
Lane 1: HCT-116 cell lysate at 10 µg
Lane 2: A549 cell lysate at 10 µg
SecondaryAll lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img152323.jpg "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/20000 dilution
Lane 1: NIH/3T3 cell lysate at 20 µg
Lane 2: PC-12 cell lysate at 20 µg
SecondaryAll lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img112165.jpg "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/1000 dilution
Lane 1: A549 cell lysate at 10 µg
Lane 2: HCT-116 cell lysate at 10 µg
Lane 3: NIH/3T3 cell lysate at 10 µg
Lane 4: PC-12 cell lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 38 kDa
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img94717.jpg "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641) at 1/1000 dilution
All lanes: HepG2 cell lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33318.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with purified Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108223.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with unpurified Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--immunocytochemistry-immunofluorescence-img37815.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
This data was developed using Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling Cathepsin L with purified Anti-Cathepsin L/V/K/H antibody [EPR8011] ab133641 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/500) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
![OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--oi-rd-scanning-img84191.jpg "OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img83583.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img122816.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img152323.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img112165.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--western-blot-img94717.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33318.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108223.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--immunocytochemistry-immunofluorescence-img37815.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
![OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589), expandable thumbnail](https://content.abcam.com/products/images/cathepsin-l-v-k-h-antibody-epr8011-bsa-and-azide-free-ab248589--oi-rd-scanning-img84191.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)")
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