JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB153737

Anti-Cathepsin S antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal Cathepsin S antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CTSS aa 100 to C-terminus.

View Alternative Names

Cathepsin S, CTSS

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin S antibody (AB153737)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin S antibody (AB153737)

Confocal Immunofluorescent analysis of methanol-fixed HeLa cells labeling Cathepsin S with ab153737 at 1/500 dilution. Alpha-tubulin filaments are labeled red.

Western blot - Anti-Cathepsin S antibody (AB153737)
  • WB

Supplier Data

Western blot - Anti-Cathepsin S antibody (AB153737)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cathepsin S antibody (ab153737) at 1/1000 dilution

Lane 1:

A549 whole cell extracts with starvation, 24hr at 30 µg

Lane 2:

A549 whole cell extracts with starvation, 24hr treated with 100ng/ml IFN-gamma, 48hr at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 37 kDa

false

Western blot - Anti-Cathepsin S antibody (AB153737)
  • WB

Supplier Data

Western blot - Anti-Cathepsin S antibody (AB153737)

Samples were separated by 15% SDS-PAGE. The signal was developed with Trident ECL plus Enhanced.

All lanes:

Western blot - Anti-Cathepsin S antibody (ab153737) at 1/1000 dilution

All lanes:

Capan 2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 37 kDa

true

Western blot - Anti-Cathepsin S antibody (AB153737)
  • WB

Unknown

Western blot - Anti-Cathepsin S antibody (AB153737)

10% SDS PAGE

All lanes:

Western blot - Anti-Cathepsin S antibody (ab153737) at 1/500 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CTSS aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25774

style
left-column

Alternative Products

Primary Antibodies

AB232740

Anti-Cathepsin S antibody

Western blot - Anti-Cathepsin S antibody (AB232740)

  • 0
  • 0
Primary Antibodies

AB134157

Anti-Cathepsin S antibody [EPR5128]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin S antibody [EPR5128] (AB134157)

  • 4
  • 1

Complementary Products

Assay Kits

AB65307

Cathepsin S Activity Assay Kit (Fluorometric)

Functional Studies - Cathepsin S Activity Assay Kit (Fluorometric) (AB65307)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB125067

Anti-Cathepsin B antibody [EPR4323]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (AB125067)

  • 0
  • 0
Primary Antibodies

AB133641

Anti-Cathepsin L/V/K/H antibody [EPR8011]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (AB133641)

  • 5
  • 6
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin S sometimes referred to as CTSS is a cysteine protease enzyme weighing about 24-29 kDa part of the papain family. It occurs mainly in lysosomes and is expressed in a variety of cells including antigen-presenting cells like macrophages dendritic cells and B lymphocytes. Cathepsin S functions to cleave proteins at lysines playing a central role in protein degradation and antigen processing. It distinguishes itself from other cathepsins by maintaining activity in an alkaline environment and is essential for the processing of invariant chain (Ii) in MHC class II molecules.
Biological function summary

Cathepsin S participates in immune system modulation and matrix degradation. It stands out in its role in the immune response cleaving antigenic proteins for presentation by MHC class II therefore influencing T-cell activation. Although synonymous with lysosomal activity it also operates extracellularly contributing to tissue remodeling and inflammation. Cathepsin S is not part of a larger complex but interacts with MHC class II impacting antigen presentation.

Pathways

This protease is critical in the immune system and extracellular matrix degradation pathways. In the context of the immune pathway CTSS interacts with other proteases such as Cathepsin L and Cathepsin B to perform antigen trimming and processing. Moreover CTSS supports the activation of T lymphocytes by ensuring the correct presentation of peptide fragments on MHC class II molecules. In matrix degradation CTSS indirectly influences collagen breakdown and interaction with other matrix metalloproteinases.

Cathepsin S is associated with autoimmune diseases like rheumatoid arthritis where it contributes to tissue inflammation and joint damage. It also relates to atherosclerosis promoting instability in atherosclerotic plaques which could result in heart disease. In rheumatoid arthritis interactions with cytokines like TNF-alpha amplify inflammatory responses while in atherosclerosis CTSS links with other proteases to degrade extracellular matrix components leading to vulnerable lesions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Thiol protease (PubMed : 30612035). Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation (PubMed : 30612035). The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L (PubMed : 30612035). Elicits itch by mediating cleavage and activation of MRGPRX1 (By similarity).
See full target information CTSS
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2017:4786170 PubMed29362520

2017

Role of Cathepsin S in Periodontal Inflammation and Infection.

Applications

ICC/IF

Species

Human

S Memmert,A Damanaki,A V B Nogueira,S Eick,M Nokhbehsaim,A K Papadopoulou,A Till,B Rath,S Jepsen,W Götz,C Piperi,E K Basdra,J A Cirelli,A Jäger,J Deschner
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com