AB232740

Anti-Cathepsin S antibody

Name: Anti-Cathepsin S antibody (ab232740) | Abcam
Brand: Abcam Limited
SKU: AB232740

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(10 Publications)

Rabbit Polyclonal Cathepsin S antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Recombinant full length protein - Cow samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Cow CTSS aa 100 to C-terminus.

View Alternative Names

Cats, Ctss, Cathepsin S

3 Images

Supplier Data

Western blot - Anti-Cathepsin S antibody (AB232740)

All lanes:

Western blot - Anti-Cathepsin S antibody (ab232740) at 2 µg/mL

All lanes:

Mouse liver lysate.

Predicted band size: 37 kDa

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Supplier Data

Western blot - Anti-Cathepsin S antibody (AB232740)

All lanes:

Western blot - Anti-Cathepsin S antibody (ab232740) at 2 µg/mL

All lanes:

Recombinant cow Cathepsin S protein.

Predicted band size: 37 kDa

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Supplier Data

Western blot - Anti-Cathepsin S antibody (AB232740)

All lanes:

Western blot - Anti-Cathepsin S antibody (ab232740) at 1 µg/mL

All lanes:

Pig spleen tissue lysate

Predicted band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Cow CTSS aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25326

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin S sometimes referred to as CTSS is a cysteine protease enzyme weighing about 24-29 kDa part of the papain family. It occurs mainly in lysosomes and is expressed in a variety of cells including antigen-presenting cells like macrophages dendritic cells and B lymphocytes. Cathepsin S functions to cleave proteins at lysines playing a central role in protein degradation and antigen processing. It distinguishes itself from other cathepsins by maintaining activity in an alkaline environment and is essential for the processing of invariant chain (Ii) in MHC class II molecules.

Biological function summary

Cathepsin S participates in immune system modulation and matrix degradation. It stands out in its role in the immune response cleaving antigenic proteins for presentation by MHC class II therefore influencing T-cell activation. Although synonymous with lysosomal activity it also operates extracellularly contributing to tissue remodeling and inflammation. Cathepsin S is not part of a larger complex but interacts with MHC class II impacting antigen presentation.

Pathways

This protease is critical in the immune system and extracellular matrix degradation pathways. In the context of the immune pathway CTSS interacts with other proteases such as Cathepsin L and Cathepsin B to perform antigen trimming and processing. Moreover CTSS supports the activation of T lymphocytes by ensuring the correct presentation of peptide fragments on MHC class II molecules. In matrix degradation CTSS indirectly influences collagen breakdown and interaction with other matrix metalloproteinases.

Cathepsin S is associated with autoimmune diseases like rheumatoid arthritis where it contributes to tissue inflammation and joint damage. It also relates to atherosclerosis promoting instability in atherosclerotic plaques which could result in heart disease. In rheumatoid arthritis interactions with cytokines like TNF-alpha amplify inflammatory responses while in atherosclerosis CTSS links with other proteases to degrade extracellular matrix components leading to vulnerable lesions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.

See full target information Ctss

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0319053 PubMed39964999

2025

Proteomic and metabolomic profiling reveals the underlying molecular mechanisms in modified alternate-day fasting-mediated protection against Diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zeng,Yi-Hang Xing,Xiu-Mei Ma,Yang Long,Zong-Zhe Jiang,Yong Xu

Scientific reports 15:2152 PubMed39820824

2025

Comprehensive investigation of matrix metalloproteinases in skin cutaneous melanoma: diagnostic, prognostic, and therapeutic insights.

Applications

Unspecified application

Species

Unspecified reactive species

Lingxia Wu,Chenxiaoxiao Liu,Weicai Hu

American journal of translational research 16:544-556 PubMed38463588

2024

Dysregulation of kidney proteases in the pathogenesis of hypertension following unilateral nephrectomy in juvenile mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha Aly,Yunus E Dogan,Niharika Bala,Carlos Lugo,Seena Darwish,Lawrence Shoemaker,Abdel A Alli

International journal of molecular sciences 24: PubMed37569859

2023

Augmentation of Cathepsin Isoforms in Diabetic db/db Mouse Kidneys Is Associated with an Increase in Renal MARCKS Expression and Proteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed F Gholam,Niharika Bala,Yunus E Dogan,Abdel A Alli

Annals of translational medicine 10:1172 PubMed36467351

2022

Cathepsin S inhibitor reduces high-fat-induced adipogenesis, inflammatory infiltration, and hepatic lipid accumulation in obese mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zheng,Huijun Zhuang,Tian Zhang,Yanni Wang,Ting Ran,Juan He,Na Han,Juan Duan

Nature immunology 23:705-717 PubMed35487985

2022

Caspase-11 interaction with NLRP3 potentiates the noncanonical activation of the NLRP3 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Moretti,Baosen Jia,Zachary Hutchins,Soumit Roy,Hilary Yip,Jiahui Wu,Meimei Shan,Samie R Jaffrey,Jörn Coers,J Magarian Blander

Aging 13:15638-15658 PubMed34077394

2021

Transfer of exosomal microRNA-203-3p from dendritic cells to bone marrow-derived macrophages reduces development of atherosclerosis by downregulating Ctss in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Beiyou Lin,Wenchao Xie,Chunmei Zeng,Xiaodan Wu,Ang Chen,Hao Li,Rina Jiang,Ping Li

The Journal of endocrinology 248:167-179 PubMed33289685

2020

Circulating cathepsin S improves glycaemic control in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hamzeh Karimkhanloo,Stacey N Keenan,Emily W Sun,David A Wattchow,Damien J Keating,Magdalene K Montgomery,Matthew J Watt

Journal of cellular physiology 236:1309-1320 PubMed32657442

2020

Inhibition of cathepsin S attenuates myocardial ischemia/reperfusion injury by suppressing inflammation and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Peng,Hong Liu,Bin Yan,Xiao-Wen Meng,Shao-Yong Song,Fu-Hai Ji,Zhengyuan Xia

Biochimie 166:94-102 PubMed31163196

2019

Stefin A-functionalized liposomes as a system for cathepsins S and L-targeted drug delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Andreja Bratovš,Lovro Kramer,Georgy Mikhaylov,Olga Vasiljeva,Boris Turk

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Cathepsin S antibody

Western blot - Anti-Cathepsin S antibody (AB232740)

Western blot - Anti-Cathepsin S antibody (AB232740)

Western blot - Anti-Cathepsin S antibody (AB232740)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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