AB166894

Anti-Cathepsin V antibody [EPR10723(B)]

Name: Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) | Abcam
Brand: Abcam Limited
SKU: AB166894
Price: 355 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal Cathepsin V antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CATL2, CTSL2, CTSU, UNQ268/PRO305, CTSV, Cathepsin L2, Cathepsin U, Cathepsin V

4 Images

Flow Cytometry (Intracellular) - Anti-Cathepsin V antibody [EPR10723(B)] (AB166894)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cathepsin V antibody [EPR10723(B)] (AB166894)

Intracellular flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (AB166894)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (AB166894)

Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution.

Unknown

Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (AB166894)

Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)

Predicted band size: 37 kDa

false

Unknown

Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (AB166894)

All lanes:

Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

SW480 cell lysate at 10 µg

Lane 4:

Fetal kidney tissue lysate at 10 µg

Lane 5:

Human small intestine tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10723(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin V also known as cathepsin L2 is a cysteine protease enzyme involved in protein degradation. This enzyme has a molecular mass of approximately 37 kDa. Cathepsin V predominantly resides in lysosomes cellular organelles known for breaking down waste materials. It is highly expressed in thymus cornea and testis suggesting its significant role in tissues that require extensive protein turnover.

Biological function summary

Cathepsin V plays essential roles in the degradation of intracellular proteins by cleaving peptide bonds. Although not part of a complex its activity helps regulate immune responses and processing of antigens keeping cellular functions normal. Cathepsin V shows high proteolytic activity which is suited for tissues where high protein breakdown and recycling are necessary.

Pathways

Cathepsin V participates in pathways linked to protein catabolism and immune system regulation. It takes part in the antigen processing and presentation pathway contributing to immune surveillance. Within these pathways its activity can interact with other proteases like cathepsin S showing functional redundancy or specificity depending on tissue type and conditions.

Cathepsin V is associated with cancer and autoimmune diseases. In some cancers its overexpression may facilitate tumor progression by degrading extracellular matrix components aiding metastasis. This upregulation can involve interaction with proteins such as cathepsin B which also modifies extracellular environments. In autoimmune disorders altered regulation of cathepsin V affects antigen processing possibly leading to abnormal immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cysteine protease. May have an important role in corneal physiology.

See full target information CTSV

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:13961-13979 PubMed38078882

2023

Cathepsin V drives lung cancer progression by shaping the immunosuppressive environment and adhesion molecules cleavage.

Applications

Unspecified application

Species

Unspecified reactive species

Lifei Zhu,Qi Zeng,Jinxiang Wang,Fan Deng,Shi Jin

Frontiers in oncology 12:876245 PubMed35494076

2022

Glycosylated Cathepsin V Serves as a Prognostic Marker in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lewei Yang,Qi Zeng,Yun Deng,Yeqing Qiu,Wei Yao,Yifeng Liao

Bioscience, biotechnology, and biochemistry 84:1436-1443 PubMed32299303

2020

Isoimperatorin (ISO) reduces melanin content in keratinocytes via miR-3619/CSTB and miR-3619/CSTD axes.

Applications

Unspecified application

Species

Unspecified reactive species

Bijun Zeng,Kai Li,Zhibo Yang,Haizhen Wang,Chang Wang,Pan Huang,Yi Pan

Cell cycle (Georgetown, Tex.) 17:844-857 PubMed29623762

2018

Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Juan Yi,Meng-Yun Su,Ying Shi,Shan Jiang,Shi-Zheng Xu,Tie-Chi Lei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cathepsin V antibody [EPR10723(B)]

Flow Cytometry (Intracellular) - Anti-Cathepsin V antibody [EPR10723(B)] (AB166894)

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (AB166894)

Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (AB166894)

Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (AB166894)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise