AB249378

Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Cathepsin V antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CATL2, CTSL2, CTSU, UNQ268/PRO305, CTSV, Cathepsin L2, Cathepsin U, Cathepsin V

4 Images

Flow Cytometry (Intracellular) - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (AB249378)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (AB249378)

This data was developed using ab166894, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (AB249378)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (AB249378)

This data was developed using ab166894, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution.

Unknown

Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (AB249378)

This data was developed using ab166894, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (<a href='/en-us/products/primary-antibodies/cathepsin-v-antibody-epr10723b-ab166894'>ab166894</a>)

Predicted band size: 37 kDa

false

Unknown

Western blot - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (AB249378)

This data was developed using ab166894, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (<a href='/en-us/products/primary-antibodies/cathepsin-v-antibody-epr10723b-ab166894'>ab166894</a>) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

SW480 cell lysate at 10 µg

Lane 4:

Fetal kidney tissue lysate at 10 µg

Lane 5:

Human small intestine tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

false

Unconjugated

Anti-Cathepsin V antibody [EPR10723(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10723(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

ab249378 is the carrier-free version of ab166894.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin V also known as cathepsin L2 is a cysteine protease enzyme involved in protein degradation. This enzyme has a molecular mass of approximately 37 kDa. Cathepsin V predominantly resides in lysosomes cellular organelles known for breaking down waste materials. It is highly expressed in thymus cornea and testis suggesting its significant role in tissues that require extensive protein turnover.

Biological function summary

Cathepsin V plays essential roles in the degradation of intracellular proteins by cleaving peptide bonds. Although not part of a complex its activity helps regulate immune responses and processing of antigens keeping cellular functions normal. Cathepsin V shows high proteolytic activity which is suited for tissues where high protein breakdown and recycling are necessary.

Pathways

Cathepsin V participates in pathways linked to protein catabolism and immune system regulation. It takes part in the antigen processing and presentation pathway contributing to immune surveillance. Within these pathways its activity can interact with other proteases like cathepsin S showing functional redundancy or specificity depending on tissue type and conditions.

Cathepsin V is associated with cancer and autoimmune diseases. In some cancers its overexpression may facilitate tumor progression by degrading extracellular matrix components aiding metastasis. This upregulation can involve interaction with proteins such as cathepsin B which also modifies extracellular environments. In autoimmune disorders altered regulation of cathepsin V affects antigen processing possibly leading to abnormal immune responses.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cysteine protease. May have an important role in corneal physiology.

See full target information CTSV

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (AB249378)

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (AB249378)

Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (AB249378)

Western blot - Anti-Cathepsin V antibody [EPR10723(B)] - BSA and Azide free (AB249378)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise