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AB191083

Anti-Cathepsin W antibody [EPR15135]

4

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal Cathepsin W antibody. Suitable for IP, WB and reacts with Human, Rat samples. Cited in 5 publications.

View Alternative Names

Cathepsin W, Lymphopain, CTSW

4 Images
Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] (AB191083)
  • IP

Supplier Data

Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] (AB191083)

Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab191083 at 1/30 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] (ab191083)

Predicted band size: 42 kDa

false

Western blot - Anti-Cathepsin W antibody [EPR15135] (AB191083)
  • WB

Supplier Data

Western blot - Anti-Cathepsin W antibody [EPR15135] (AB191083)

All lanes:

Western blot - Anti-Cathepsin W antibody [EPR15135] (ab191083) at 1/20000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

Predicted band size: 42 kDa

false

Western blot - Anti-Cathepsin W antibody [EPR15135] (AB191083)
  • WB

Supplier Data

Western blot - Anti-Cathepsin W antibody [EPR15135] (AB191083)

All lanes:

Western blot - Anti-Cathepsin W antibody [EPR15135] (ab191083) at 1/50000 dilution

Lane 1:

HuT-78 cell lysate at 20 µg

Lane 2:

MOLT-4 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

false

Western blot - Anti-Cathepsin W antibody [EPR15135] (AB191083)
  • WB

Supplier Data

Western blot - Anti-Cathepsin W antibody [EPR15135] (AB191083)

All lanes:

Western blot - Anti-Cathepsin W antibody [EPR15135] (ab191083) at 1/10000 dilution

All lanes:

PC12 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

Predicted band size: 42 kDa

false

  • Carrier free

    Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15135

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin W also known as CTSW is a member of the cysteine protease family. It has a molecular mass of approximately 45 kDa and functions as an enzyme that breaks down protein substrates through proteolysis. CTSW displays specificity for certain peptide bonds in its target proteins. This protein is mostly expressed in the immune system especially in cytotoxic T cells and natural killer cells. The expression pattern suggests its involvement in immune responses.
Biological function summary

Cathepsin W contributes to the modulation of cytotoxic activity. It assists in the rearrangement and processing of proteins important for the immune response facilitating the proper functioning of immune effector cells. While not part of a specific protein complex CTSW activity aligns with immune cell regulation mechanisms. Its role in the degradation of proteins plays a significant part in maintaining immune cell competency.

Pathways

Cathepsin W contributes to the immune response and apoptosis. The immune response pathway involves cytotoxic T cells and natural killer cells where CTSW enhances the degradation of target proteins. In apoptosis the protein functions in the breakdown of cellular components that need recycling or removal. Cathepsin W shares functional similarities with related proteases such as Cathepsin C which also participates in immune cell regulation and apoptosis.

Cathepsin W has relevance in immunological diseases and some cancer types. Altered activity of CTSW may associate with impaired immune responses contributing to immunodeficiency conditions. In certain cancers such as leukemia changes in CTSW expression could influence the behavior of tumor-infiltrating lymphocytes. Proteins like granzyme B which are involved in inducing apoptosis might interact with CTSW reflecting their shared pathways in modulating immune responses and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May have a specific function in the mechanism or regulation of T-cell cytolytic activity.. (Microbial infection) Plays a role during influenza virus infection in lungs cells ex vivo. Acts at the level of virus entering host cytoplasm from late endosome.
See full target information CTSW
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:1017 PubMed39529122

2024

Compromised C3b-VSIG4 axis between decidual NK cells and macrophages contributes to recurrent spontaneous abortion.

Applications

Unspecified application

Species

Unspecified reactive species

Siao Chen,Jinghe Zhang,Jian Chen,Jieqi Ke,Yu Huang,Xianghui Du,Binqing Fu,Haiming Wei

Toxicology research 13:tfae096 PubMed38957783

2024

LncRNA HILPDA promotes contrast-induced acute kidney injury by recruiting eIF4B to upregulate XPO1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Wei,Tuersun Tilakezi,Wei Feng,Heyin Yang,Shujun Yang

Asia-Pacific journal of clinical oncology 21:77-86 PubMed37415393

2023

C-reactive protein is a prognostic biomarker in pancreatic ductal adenocarcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa F Bonazzi,Lauren G Aoude,Sandra Brosda,Julia J Bradford,James M Lonie,Kelly A Loffler,Michael G Gartside,Kalpana Patel,Pamela Mukhopadhyay,Colm Keane,Val Gebski,James G Kench,David Goldstein,Nicola Waddell,Andrew P Barbour

Frontiers in immunology 14:1161382 PubMed37180113

2023

A novel prognostic signatures based on metastasis- and immune-related gene pairs for colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Pan,Yanzhe Yue,Wenbo Ding,Li Sun,Mu Xu,Shukui Wang

Cancers 12: PubMed32455715

2020

Cysteine Cathepsins Inhibition Affects Their Expression and Human Renal Cancer Cell Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Rudzińska,Alessandro Parodi,Valentina D Maslova,Yuri M Efremov,Neonila V Gorokhovets,Vladimir A Makarov,Vasily A Popkov,Andrey V Golovin,Evgeni Y Zernii,Andrey A Zamyatnin
View all publications
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Product promise

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