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AB251056

Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free

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Rabbit Recombinant Monoclonal Cathepsin W antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat samples.

View Alternative Names

Cathepsin W, Lymphopain, CTSW

4 Images
Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)
  • WB

Supplier Data

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)

This data was developed using ab191083, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cathepsin W antibody [EPR15135] (<a href='/en-us/products/primary-antibodies/cathepsin-w-antibody-epr15135-ab191083'>ab191083</a>) at 1/20000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

Predicted band size: 42 kDa

false

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)
  • WB

Supplier Data

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)

This data was developed using ab191083, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cathepsin W antibody [EPR15135] (<a href='/en-us/products/primary-antibodies/cathepsin-w-antibody-epr15135-ab191083'>ab191083</a>) at 1/50000 dilution

Lane 1:

HuT-78 cell lysate at 20 µg

Lane 2:

MOLT-4 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

false

Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)
  • IP

Supplier Data

Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)

This data was developed using ab191083, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab191083 at 1/30 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] (<a href='/en-us/products/primary-antibodies/cathepsin-w-antibody-epr15135-ab191083'>ab191083</a>)

Predicted band size: 42 kDa

false

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)
  • WB

Supplier Data

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)

This data was developed using ab191083, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cathepsin W antibody [EPR15135] (<a href='/en-us/products/primary-antibodies/cathepsin-w-antibody-epr15135-ab191083'>ab191083</a>) at 1/10000 dilution

All lanes:

PC12 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15135

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251056 is the carrier-free version of ab191083.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin W also known as CTSW is a member of the cysteine protease family. It has a molecular mass of approximately 45 kDa and functions as an enzyme that breaks down protein substrates through proteolysis. CTSW displays specificity for certain peptide bonds in its target proteins. This protein is mostly expressed in the immune system especially in cytotoxic T cells and natural killer cells. The expression pattern suggests its involvement in immune responses.
Biological function summary

Cathepsin W contributes to the modulation of cytotoxic activity. It assists in the rearrangement and processing of proteins important for the immune response facilitating the proper functioning of immune effector cells. While not part of a specific protein complex CTSW activity aligns with immune cell regulation mechanisms. Its role in the degradation of proteins plays a significant part in maintaining immune cell competency.

Pathways

Cathepsin W contributes to the immune response and apoptosis. The immune response pathway involves cytotoxic T cells and natural killer cells where CTSW enhances the degradation of target proteins. In apoptosis the protein functions in the breakdown of cellular components that need recycling or removal. Cathepsin W shares functional similarities with related proteases such as Cathepsin C which also participates in immune cell regulation and apoptosis.

Cathepsin W has relevance in immunological diseases and some cancer types. Altered activity of CTSW may associate with impaired immune responses contributing to immunodeficiency conditions. In certain cancers such as leukemia changes in CTSW expression could influence the behavior of tumor-infiltrating lymphocytes. Proteins like granzyme B which are involved in inducing apoptosis might interact with CTSW reflecting their shared pathways in modulating immune responses and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May have a specific function in the mechanism or regulation of T-cell cytolytic activity.. (Microbial infection) Plays a role during influenza virus infection in lungs cells ex vivo. Acts at the level of virus entering host cytoplasm from late endosome.
See full target information CTSW
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com