AB182575

Anti-Cathepsin Z antibody [EPR14357-7]

Name: Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) | Abcam
Brand: Abcam Limited
SKU: AB182575
Price: 540 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal Cathepsin Z antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Cathepsin Z, Cathepsin P, Cathepsin X, CTSZ

4 Images

Flow Cytometry (Intracellular) - Anti-Cathepsin Z antibody [EPR14357-7] (AB182575)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cathepsin Z antibody [EPR14357-7] (AB182575)

Intracellular flow cytometric analysis of 2%paraformaldehyde-fixedHeLa cells labeling Cathepsin Z with ab182575 at 1/80 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibodyat 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-Cathepsin Z antibody [EPR14357-7] (AB182575)

Western blot analysis of Cathepsin Z in immunoprecipitation pellets from WI-38 lysate immunoprecipitated using ab182575 at 1/50 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Cathepsin Z antibody [EPR14357-7] (ab182575)

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-Cathepsin Z antibody [EPR14357-7] (AB182575)

All lanes:

Western blot - Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) at 1/5000 dilution

All lanes:

WI-38 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 20 µg

Predicted band size: 33 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-Cathepsin Z antibody [EPR14357-7] (AB182575)

All lanes:

Western blot - Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) at 1/2000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

HeLa lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 34 kDa

false

Carrier free

Anti-Cathepsin Z antibody [EPR14357-7] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14357-7

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20 - 1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/80", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin Z also known as cathepsin X or CTSZ is a lysosomal cysteine protease with a molecular weight of approximately 34 kDa. It is expressed mainly in immune cells such as macrophages and dendritic cells but also found in other tissues including liver kidney and spleen. This protease performs proteolytic functions which involve cleaving peptide bonds in proteins contributing to protein catabolism in cells. The widespread expression of Cathepsin Z suggests its involvement in various physiological processes.

Biological function summary

Cathepsin Z participates in immune response regulation and cellular adhesion processes. It belongs to the papain-like cysteine protease family. Notably it associates with integrins to influence cell-extracellular matrix interactions affecting cell migration and adhesion. By modulating these processes Cathepsin Z impacts immune surveillance and tissue remodeling demonstrating its multifaceted role in maintaining cellular homeostasis.

Pathways

Cathepsin Z engages significantly in apoptotic and antigen-presenting pathways. In apoptosis it influences cell death by degrading specific substrates involved in cell survival. It also assists in the processing of antigens within dendritic cells facilitating effective antigen presentation to T cells. Related proteins such as Cathepsin S and Cathepsin L which also partake in lysosomal pathways further illustrate the shared functionality and redundancy within this protease family enabling a robust regulatory network.

Cathepsin Z has implications in cancer progression and neurodegenerative diseases. It is often overexpressed in cancerous tissues where it aids tumor cell invasion and metastasis through degradation of extracellular components. Additionally alterations in Cathepsin Z activity are linked to Alzheimer's disease where it may affect the processing of amyloid precursor proteins similar to its interaction with other proteins like Cathepsin B. These connections highlight the potential for Cathepsin Z as a target for therapeutic intervention in various pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity (PubMed : 10504234). Capable of producing kinin potentiating peptides (By similarity).

See full target information Cathepsin Z

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1618487 PubMed40568593

2025

Prognostic and immunological implications of cathepsin Z overexpression in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junyue Tao,Yiding Chen,Xiaokang Bian,Tingting Cai,Changhao Song,Chaozhao Liang,Zongyao Hao,Jialing Meng,Qintao Ge,Jun Zhou

Frontiers in cell and developmental biology 11:1240039 PubMed37691832

2023

Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Bilekova,Balma Garcia-Colomer,Alberto Cebrian-Serrano,Silvia Schirge,Karsten Krey,Michael Sterr,Thomas Kurth,Stefanie M Hauck,Heiko Lickert

Kidney360 4:1080-1094 PubMed37222594

2023

Energy Metabolism Dysregulation in Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Li,Wanjun Gu,Mark Hepokoski,Hai Pham,Rick Tham,Young Chul Kim,Tatum S Simonson,Prabhleen Singh

Molecular neurobiology 60:807-819 PubMed36370154

2022

Insight into the Neuroprotective Effect of Genistein-3'-Sodium Sulfonate Against Neonatal Hypoxic-Ischaemic Brain Injury in Rats by Bioinformatics.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xie,Liyan Shuang,Gaigai Liu,Shanshan Zhao,Zhidong Yuan,Hao Cai,Lixia Jiang,Zhihua Huang

Cell death & disease 13:386 PubMed35444189

2022

The caspase-2 substrate p54nrb exhibits a multifaceted role in tumor cell death susceptibility via gene regulatory functions.

Applications

Unspecified application

Species

Unspecified reactive species

Madeleine Eichler,Ute Distler,Usman Nasrullah,Aswini Krishnan,Manuel Kaulich,Koraljka Husnjak,Wolfgang Eberhardt,Krishnaraj Rajalingam,Stefan Tenzer,Josef Pfeilschifter,Gergely Imre

BioMed research international 2022:7858477 PubMed35155681

2022

Establishment and External Validation of a Hypoxia-Derived Gene Signature for Robustly Predicting Prognosis and Therapeutic Responses in Glioblastoma Multiforme.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Wang,Yang Lu,Zhihong Liu,Min Diao,Lin Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cathepsin Z antibody [EPR14357-7]

Flow Cytometry (Intracellular) - Anti-Cathepsin Z antibody [EPR14357-7] (AB182575)

Immunoprecipitation - Anti-Cathepsin Z antibody [EPR14357-7] (AB182575)

Western blot - Anti-Cathepsin Z antibody [EPR14357-7] (AB182575)

Western blot - Anti-Cathepsin Z antibody [EPR14357-7] (AB182575)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise