Anti-CaV1.3 antibody [S48]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [S48] (AB85491)

ab85491 staining CaV1.3 in human hippocampus tissue by IHC-P (Bouin's fixed paraffin embedded tissue sections). The primary antibody was used at 1 : 1000 for 1 hour at RT. Secondary Antibody : FITC Goat Anti-Mouse (green) at 1 : 50 for 1 hour at RT.

• Flow Cyt

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Flow Cytometry - Anti-CaV1.3 antibody [S48] (AB85491)

Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab85491 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85491, 0.5 μg/1x10⁶ cells) for 30 minutes at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1 μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween for 20 minutes used under the same conditions.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [S48] (AB85491)

ab85491 staining CaV1.3 in mouse backskin tissue by IHC-P (Bouin's fixed paraffin embedded tissue sections).

Tissue underwent antigen retrieval using microwave in citrate buffer. The primary antibody was used at 1/100 dilution and then sections were incubated with Fluorophore conjugated goat anti mouse at 1/50 dilution.